The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells
Aim: To study the status of the tumor growth factor beta (TGFB) pathway in chronic lymphocytic leukemia (CLL) cells and to uncover molecular details underlying CLL cell genesis. Objects and Methods: The study was conducted on peripheral blood samples of patients with CLL using the following methods:...
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| Published in: | Experimental Oncology |
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| Date: | 2017 |
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/138586 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells / A. Matveeva, L. Kovalevska, I. Kholodnyuk, T. Ivanivskaya, E. Kashuba // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 286–290. — Бібліогр.: 26 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862731739186593792 |
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| author | Matveeva, A. Kovalevska, L. Kholodnyuk, I. Ivanivskaya, T. Kashuba, E. |
| author_facet | Matveeva, A. Kovalevska, L. Kholodnyuk, I. Ivanivskaya, T. Kashuba, E. |
| citation_txt | The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells / A. Matveeva, L. Kovalevska, I. Kholodnyuk, T. Ivanivskaya, E. Kashuba // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 286–290. — Бібліогр.: 26 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To study the status of the tumor growth factor beta (TGFB) pathway in chronic lymphocytic leukemia (CLL) cells and to uncover molecular details underlying CLL cell genesis. Objects and Methods: The study was conducted on peripheral blood samples of patients with CLL using the following methods: RNA isolation, analysis of expression of transcription factors using RT2 profiler assay, bioinformatics analysis of publicly available data bases on expression. Results: We have shown that the TGFB — SMAD canonical pathway is not active in CLL cells. SMAD-responsive genes, such as BCL2L1 (BCL-XL), CCND2 (Cyclin D2), and MYC, are down-regulated in CLL cells compared with peripheral blood B cells of healthy donors. Conclusions: The TGFB-mediated signaling is not active in CLL cells due to low (or absent) expression of SMAD1, -4, -5, -9, and ATF-3. Expression and phosphorylation status of SMAD2 and -3 should be further elucidated in the future studies.
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| first_indexed | 2025-12-07T19:27:41Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-138586 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T19:27:41Z |
| publishDate | 2017 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Matveeva, A. Kovalevska, L. Kholodnyuk, I. Ivanivskaya, T. Kashuba, E. 2018-06-19T10:03:11Z 2018-06-19T10:03:11Z 2017 The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells / A. Matveeva, L. Kovalevska, I. Kholodnyuk, T. Ivanivskaya, E. Kashuba // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 286–290. — Бібліогр.: 26 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138586 Aim: To study the status of the tumor growth factor beta (TGFB) pathway in chronic lymphocytic leukemia (CLL) cells and to uncover molecular details underlying CLL cell genesis. Objects and Methods: The study was conducted on peripheral blood samples of patients with CLL using the following methods: RNA isolation, analysis of expression of transcription factors using RT2 profiler assay, bioinformatics analysis of publicly available data bases on expression. Results: We have shown that the TGFB — SMAD canonical pathway is not active in CLL cells. SMAD-responsive genes, such as BCL2L1 (BCL-XL), CCND2 (Cyclin D2), and MYC, are down-regulated in CLL cells compared with peripheral blood B cells of healthy donors. Conclusions: The TGFB-mediated signaling is not active in CLL cells due to low (or absent) expression of SMAD1, -4, -5, -9, and ATF-3. Expression and phosphorylation status of SMAD2 and -3 should be further elucidated in the future studies. We thank Professor Danylo Gluzman (R.E. Kavetsky IEPOR of NAS of Ukraine) for the fruitful discussions over this manuscript. This work was supported by the Swedish Cancer Society, by matching grants from the Concern Foundation (Los Angeles, USA) and the Cancer Research Institute (New York, USA), and by the Academy of Science of Ukraine (grant No. 0112U002192). en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells Article published earlier |
| spellingShingle | The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells Matveeva, A. Kovalevska, L. Kholodnyuk, I. Ivanivskaya, T. Kashuba, E. Original contributions |
| title | The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells |
| title_full | The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells |
| title_fullStr | The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells |
| title_full_unstemmed | The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells |
| title_short | The TGF-beta — SMAD pathway is inactivated in cronic lymphocytic leukemia cells |
| title_sort | tgf-beta — smad pathway is inactivated in cronic lymphocytic leukemia cells |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138586 |
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