Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro

Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro

Aim: To synthesize a conjugate of hematoporphyrin with gold nanoparticles, and to evaluate its photodynamic activity in experiments on cultures of transformed cells. Methods: nanosized gold particles and nanocomposites synthesis methods, cell culture methods, photobiology methods, trypan blue dye ex...

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Veröffentlicht in:Experimental Oncology
Datum:2010
Hauptverfasser: Gamaleia, N.F., Shishko, E.D., Dolinsky, G.A., Shcherbakov, A.B., Usatenko, A.V., Kholin, V.V.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2010
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/138590
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Zitieren:Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro / N.F. Gamaleia, E.D. Shishko, G.A. Dolinsky, A.B. Shcherbakov, A.V. Usatenko, V.V. Kholin // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 44-47. — Бібліогр.: 27 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138590
record_format dspace
spelling Gamaleia, N.F.
Shishko, E.D.
Dolinsky, G.A.
Shcherbakov, A.B.
Usatenko, A.V.
Kholin, V.V.
2018-06-19T10:11:46Z
2018-06-19T10:11:46Z
2010
Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro / N.F. Gamaleia, E.D. Shishko, G.A. Dolinsky, A.B. Shcherbakov, A.V. Usatenko, V.V. Kholin // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 44-47. — Бібліогр.: 27 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138590
Aim: To synthesize a conjugate of hematoporphyrin with gold nanoparticles, and to evaluate its photodynamic activity in experiments on cultures of transformed cells. Methods: nanosized gold particles and nanocomposites synthesis methods, cell culture methods, photobiology methods, trypan blue dye exclusion test, chemiluminescence assay. Results: Various hematoporphyrin-gold nanocomposites were obtained, which contained similar hematoporphyrin concentration (5 μg/ml) and varied concentrations (0.5–5 μg/ml) of gold nanoparticles with a diameter of 15 nm or 45 nm. It was established by chemiluminescence method that nanocomposites synthesized induce more efficiently the formation of photo-oxidative products than original photosensitizer. The experiments with transformed cell lines showed that photodynamic in vitro activity of synthesized hematoporphyrin-nanogold composites is much higher than that of the original photosensitizer. The better activity of the nanocomposites with gold particles of 45 nm vs such of 15 nm which was demonstrated in the experiments, can be apparently connected with the fact that bigger particles are able to transport more porphyrin molecules into malignant cells. Conclusion: The results obtained warrant the necessity of further studies with hematoporphyrin-gold nanocomposites in vivo on transplanted tumors of animals which have to define the real perspectives of the nanocomposites application in PDT.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro
spellingShingle Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro
Gamaleia, N.F.
Shishko, E.D.
Dolinsky, G.A.
Shcherbakov, A.B.
Usatenko, A.V.
Kholin, V.V.
Original contributions
title_short Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro
title_full Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro
title_fullStr Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro
title_full_unstemmed Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro
title_sort photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro
author Gamaleia, N.F.
Shishko, E.D.
Dolinsky, G.A.
Shcherbakov, A.B.
Usatenko, A.V.
Kholin, V.V.
author_facet Gamaleia, N.F.
Shishko, E.D.
Dolinsky, G.A.
Shcherbakov, A.B.
Usatenko, A.V.
Kholin, V.V.
topic Original contributions
topic_facet Original contributions
publishDate 2010
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To synthesize a conjugate of hematoporphyrin with gold nanoparticles, and to evaluate its photodynamic activity in experiments on cultures of transformed cells. Methods: nanosized gold particles and nanocomposites synthesis methods, cell culture methods, photobiology methods, trypan blue dye exclusion test, chemiluminescence assay. Results: Various hematoporphyrin-gold nanocomposites were obtained, which contained similar hematoporphyrin concentration (5 μg/ml) and varied concentrations (0.5–5 μg/ml) of gold nanoparticles with a diameter of 15 nm or 45 nm. It was established by chemiluminescence method that nanocomposites synthesized induce more efficiently the formation of photo-oxidative products than original photosensitizer. The experiments with transformed cell lines showed that photodynamic in vitro activity of synthesized hematoporphyrin-nanogold composites is much higher than that of the original photosensitizer. The better activity of the nanocomposites with gold particles of 45 nm vs such of 15 nm which was demonstrated in the experiments, can be apparently connected with the fact that bigger particles are able to transport more porphyrin molecules into malignant cells. Conclusion: The results obtained warrant the necessity of further studies with hematoporphyrin-gold nanocomposites in vivo on transplanted tumors of animals which have to define the real perspectives of the nanocomposites application in PDT.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138590
citation_txt Photodynamic activity of hematoporphyrin conjugates with gold nanoparticles: experiments in vitro / N.F. Gamaleia, E.D. Shishko, G.A. Dolinsky, A.B. Shcherbakov, A.V. Usatenko, V.V. Kholin // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 44-47. — Бібліогр.: 27 назв. — англ.
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AT dolinskyga photodynamicactivityofhematoporphyrinconjugateswithgoldnanoparticlesexperimentsinvitro
AT shcherbakovab photodynamicactivityofhematoporphyrinconjugateswithgoldnanoparticlesexperimentsinvitro
AT usatenkoav photodynamicactivityofhematoporphyrinconjugateswithgoldnanoparticlesexperimentsinvitro
AT kholinvv photodynamicactivityofhematoporphyrinconjugateswithgoldnanoparticlesexperimentsinvitro
first_indexed 2025-12-07T20:39:46Z
last_indexed 2025-12-07T20:39:46Z
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