Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes
Aim: To estimate the viability of normal and transformed T-lymphocytes after UV/Vis irradiation in the presence of pristine fullerenes C60. Methods: Thymocytes were isolated from Wistar rats’ thymus. Murine leukemia L1210 and human lymphoma Jurkat cells were used in this study. Mercury-vapor lamp wa...
Gespeichert in:
| Veröffentlicht in: | Experimental Oncology |
|---|---|
| Datum: | 2010 |
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | English |
| Veröffentlicht: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2010
|
| Schlagworte: | |
| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/138592 |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes / S.V. Prylutska, I.I. Grynyuk, K.O. Palyvoda, O.P. Matyshevska // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 29-32. — Бібліогр.: 15 назв. — англ. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
nasplib_isofts_kiev_ua-123456789-138592 |
|---|---|
| record_format |
dspace |
| spelling |
Prylutska, S.V. Grynyuk, I.I. Palyvoda, K.O. Matyshevska, O.P. 2018-06-19T10:13:26Z 2018-06-19T10:13:26Z 2010 Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes / S.V. Prylutska, I.I. Grynyuk, K.O. Palyvoda, O.P. Matyshevska // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 29-32. — Бібліогр.: 15 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138592 Aim: To estimate the viability of normal and transformed T-lymphocytes after UV/Vis irradiation in the presence of pristine fullerenes C60. Methods: Thymocytes were isolated from Wistar rats’ thymus. Murine leukemia L1210 and human lymphoma Jurkat cells were used in this study. Mercury-vapor lamp was used for fullerenes C60 photoexcitation. Cytotoxicity was etermined by MTT assay. Changes in cell morphology were monitored by phase-contrast light microscopy. Results: fullerenes C60 exhibit cytotoxic effect against transformed T-lymphocytes when combined with UV/Vis irradiation (320–600 nm). Photoinduced effect was enhanced with the increasing of irradiation time period and C60 concentration, cell death was registered after 24 hours incubation. Fullerenes C60 photocytotoxicity against normal T-lymphocytes (thymocytes) was not observed. Conclusion: The present study suggests that pristine fullerenes C60 have the potential to be an effective photosensitizer and exhibit cytotoxic effect on transformed T-cells in vitro. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes |
| spellingShingle |
Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes Prylutska, S.V. Grynyuk, I.I. Palyvoda, K.O. Matyshevska, O.P. Original contributions |
| title_short |
Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes |
| title_full |
Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes |
| title_fullStr |
Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes |
| title_full_unstemmed |
Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes |
| title_sort |
photoinduced cytotoxic effect of fullerenes c60 on transformed t-lymphocytes |
| author |
Prylutska, S.V. Grynyuk, I.I. Palyvoda, K.O. Matyshevska, O.P. |
| author_facet |
Prylutska, S.V. Grynyuk, I.I. Palyvoda, K.O. Matyshevska, O.P. |
| topic |
Original contributions |
| topic_facet |
Original contributions |
| publishDate |
2010 |
| language |
English |
| container_title |
Experimental Oncology |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| format |
Article |
| description |
Aim: To estimate the viability of normal and transformed T-lymphocytes after UV/Vis irradiation in the presence of pristine fullerenes C60. Methods: Thymocytes were isolated from Wistar rats’ thymus. Murine leukemia L1210 and human lymphoma Jurkat cells were used in this study. Mercury-vapor lamp was used for fullerenes C60 photoexcitation. Cytotoxicity was etermined by MTT assay. Changes in cell morphology were monitored by phase-contrast light microscopy. Results: fullerenes C60 exhibit cytotoxic effect against transformed T-lymphocytes when combined with UV/Vis irradiation (320–600 nm). Photoinduced effect was enhanced with the increasing of irradiation time period and C60 concentration, cell death was registered after 24 hours incubation. Fullerenes C60 photocytotoxicity against normal T-lymphocytes (thymocytes) was not observed. Conclusion: The present study suggests that pristine fullerenes C60 have the potential to be an effective photosensitizer and exhibit cytotoxic effect on transformed T-cells in vitro.
|
| issn |
1812-9269 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/138592 |
| citation_txt |
Photoinduced cytotoxic effect of fullerenes C60 on transformed T-lymphocytes / S.V. Prylutska, I.I. Grynyuk, K.O. Palyvoda, O.P. Matyshevska // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 29-32. — Бібліогр.: 15 назв. — англ. |
| work_keys_str_mv |
AT prylutskasv photoinducedcytotoxiceffectoffullerenesc60ontransformedtlymphocytes AT grynyukii photoinducedcytotoxiceffectoffullerenesc60ontransformedtlymphocytes AT palyvodako photoinducedcytotoxiceffectoffullerenesc60ontransformedtlymphocytes AT matyshevskaop photoinducedcytotoxiceffectoffullerenesc60ontransformedtlymphocytes |
| first_indexed |
2025-12-01T04:09:25Z |
| last_indexed |
2025-12-01T04:09:25Z |
| _version_ |
1850859213566246912 |