Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha

Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha

Aim: To estimate the effect of long-term IFN treatment of human non-small-cell lung cancer cell line A-549 on their karyotype characteristics and on the clonal structure of cell population. Methods: Cytogenetic research was performed by standard methods using routine and differential staining. Cytog...

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Veröffentlicht in:Experimental Oncology
Datum:2010
Hauptverfasser: Kovaleva, O.A., Bezdenezhnykh, N.O., Glazko, T.T., Vorontsova, A.L., Kudryavets, Yu.I.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2010
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/138594
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Zitieren:Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha / O.A. Kovaleva, N.O. Bezdenezhnykh, T.T. Glazko, A.L. Vorontsova, Yu.I. Kudryavets // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 19-22. — Бібліогр.: 32 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138594
record_format dspace
spelling Kovaleva, O.A.
Bezdenezhnykh, N.O.
Glazko, T.T.
Vorontsova, A.L.
Kudryavets, Yu.I.
2018-06-19T10:14:51Z
2018-06-19T10:14:51Z
2010
Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha / O.A. Kovaleva, N.O. Bezdenezhnykh, T.T. Glazko, A.L. Vorontsova, Yu.I. Kudryavets // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 19-22. — Бібліогр.: 32 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138594
Aim: To estimate the effect of long-term IFN treatment of human non-small-cell lung cancer cell line A-549 on their karyotype characteristics and on the clonal structure of cell population. Methods: Cytogenetic research was performed by standard methods using routine and differential staining. Cytogenetic characteristics were estimated per 1000 cells (ppm, (‰)). Results: Cytogenetic analysis of IFN-modified A-549 human lung cancer cells had demonstrated far-going changes in their population structure. It was shown that long term cultivation with IFN altered the chromosome modal class of A-549 cells, induced the domination of hromosomes with certain molecular markers: the number of metaphases with der (6) t (6; 1) chromosomal rearrangement increased significantly (from 6% to 80%, p < 0.001) and the cells with der (2) t (2; 1) markers almost disappeared. Thus, under the effect of IFN the cell clonal selection takes place. Decrease of the cell division rate and pseudometaphase occurrence, increase of the number of cells containing micronuclei are the typical characteristics of IFN-modified А-549 cell subline A-549IFN. Conclusion: Long-term IFN effect results in alterations of cytogenetic properties of A-549 human lung cancer cells.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha
spellingShingle Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha
Kovaleva, O.A.
Bezdenezhnykh, N.O.
Glazko, T.T.
Vorontsova, A.L.
Kudryavets, Yu.I.
Original contributions
title_short Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha
title_full Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha
title_fullStr Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha
title_full_unstemmed Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha
title_sort karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha
author Kovaleva, O.A.
Bezdenezhnykh, N.O.
Glazko, T.T.
Vorontsova, A.L.
Kudryavets, Yu.I.
author_facet Kovaleva, O.A.
Bezdenezhnykh, N.O.
Glazko, T.T.
Vorontsova, A.L.
Kudryavets, Yu.I.
topic Original contributions
topic_facet Original contributions
publishDate 2010
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To estimate the effect of long-term IFN treatment of human non-small-cell lung cancer cell line A-549 on their karyotype characteristics and on the clonal structure of cell population. Methods: Cytogenetic research was performed by standard methods using routine and differential staining. Cytogenetic characteristics were estimated per 1000 cells (ppm, (‰)). Results: Cytogenetic analysis of IFN-modified A-549 human lung cancer cells had demonstrated far-going changes in their population structure. It was shown that long term cultivation with IFN altered the chromosome modal class of A-549 cells, induced the domination of hromosomes with certain molecular markers: the number of metaphases with der (6) t (6; 1) chromosomal rearrangement increased significantly (from 6% to 80%, p < 0.001) and the cells with der (2) t (2; 1) markers almost disappeared. Thus, under the effect of IFN the cell clonal selection takes place. Decrease of the cell division rate and pseudometaphase occurrence, increase of the number of cells containing micronuclei are the typical characteristics of IFN-modified А-549 cell subline A-549IFN. Conclusion: Long-term IFN effect results in alterations of cytogenetic properties of A-549 human lung cancer cells.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138594
citation_txt Karyotype alterations in human lung adenocarcinoma cells after long-term action of interferon-alpha / O.A. Kovaleva, N.O. Bezdenezhnykh, T.T. Glazko, A.L. Vorontsova, Yu.I. Kudryavets // Experimental Oncology. — 2010. — Т. 32, № 1. — С. 19-22. — Бібліогр.: 32 назв. — англ.
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first_indexed 2025-12-07T18:55:28Z
last_indexed 2025-12-07T18:55:28Z
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