Immunocytochemical study of BCR and bcr-abl localization in K562 cells
Aim: To obtain polyclonal antibodies against recombinant proteins recognizing Bcr domain and fusion region of Bcr-Abl and analyze the patterns of intracellular distribution of Bcr and Bcr-Abl proteins in K562 cells of chronic myelogenous leukemia. Methods: The coding sequences of DН and РН domains o...
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| Published in: | Experimental Oncology |
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| Date: | 2010 |
| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2010
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/138603 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Immunocytochemical study of BCR and bcr-abl localization in K562 cells / G.D. Telegeev, A.N. Dubrovska, V.A. Nadgorna, M.V. Dybkov, M.P. Zavelevich, S.S. Maliuta, D.F. Gluzman // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 81-83. — Бібліогр.: 13 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862660457902374912 |
|---|---|
| author | Telegeev, G.D. Dubrovska, A.N. Nadgorna, V.A. Dybkov, M.V. Zavelevich, M.P. Maliuta, S.S. Gluzman, D.F. |
| author_facet | Telegeev, G.D. Dubrovska, A.N. Nadgorna, V.A. Dybkov, M.V. Zavelevich, M.P. Maliuta, S.S. Gluzman, D.F. |
| citation_txt | Immunocytochemical study of BCR and bcr-abl localization in K562 cells / G.D. Telegeev, A.N. Dubrovska, V.A. Nadgorna, M.V. Dybkov, M.P. Zavelevich, S.S. Maliuta, D.F. Gluzman // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 81-83. — Бібліогр.: 13 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To obtain polyclonal antibodies against recombinant proteins recognizing Bcr domain and fusion region of Bcr-Abl and analyze the patterns of intracellular distribution of Bcr and Bcr-Abl proteins in K562 cells of chronic myelogenous leukemia. Methods: The coding sequences of DН and РН domains of Bcr-Abl were cloned, and the recombinant proteins were expressed in E. coli. The rabbit polyclonal antibodies were produced and used for immunocytochemical study of Bcr and Bcr-Abl localization in K562 cells. Results: The gene constructs containing sequences coding for DН and РН domains of Bcr-Abl have been obtained. The antibodies with relative specificity to corresponding recombinant proteins differ by the patterns of their intracellular reactivity with Bcr- and Bcr-Abl related structures. While Bcr protein is located predominantly perinuclearly, antibody against hybrid Bcr-Abl protein is reacted with the structures in cell periphery, namely on cell membranes. Conclusion: Antibodies against DН and РН domains of Bcr-Abl react with proteins located differently in chronic myelogenous leukemia cells. The difference in intracellular localization of Bcr and Bcr-Abl may be attributable to the different domains interacting with different multiprotein complexes.
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| first_indexed | 2025-12-02T10:56:44Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-138603 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-02T10:56:44Z |
| publishDate | 2010 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Telegeev, G.D. Dubrovska, A.N. Nadgorna, V.A. Dybkov, M.V. Zavelevich, M.P. Maliuta, S.S. Gluzman, D.F. 2018-06-19T10:23:34Z 2018-06-19T10:23:34Z 2010 Immunocytochemical study of BCR and bcr-abl localization in K562 cells / G.D. Telegeev, A.N. Dubrovska, V.A. Nadgorna, M.V. Dybkov, M.P. Zavelevich, S.S. Maliuta, D.F. Gluzman // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 81-83. — Бібліогр.: 13 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138603 Aim: To obtain polyclonal antibodies against recombinant proteins recognizing Bcr domain and fusion region of Bcr-Abl and analyze the patterns of intracellular distribution of Bcr and Bcr-Abl proteins in K562 cells of chronic myelogenous leukemia. Methods: The coding sequences of DН and РН domains of Bcr-Abl were cloned, and the recombinant proteins were expressed in E. coli. The rabbit polyclonal antibodies were produced and used for immunocytochemical study of Bcr and Bcr-Abl localization in K562 cells. Results: The gene constructs containing sequences coding for DН and РН domains of Bcr-Abl have been obtained. The antibodies with relative specificity to corresponding recombinant proteins differ by the patterns of their intracellular reactivity with Bcr- and Bcr-Abl related structures. While Bcr protein is located predominantly perinuclearly, antibody against hybrid Bcr-Abl protein is reacted with the structures in cell periphery, namely on cell membranes. Conclusion: Antibodies against DН and РН domains of Bcr-Abl react with proteins located differently in chronic myelogenous leukemia cells. The difference in intracellular localization of Bcr and Bcr-Abl may be attributable to the different domains interacting with different multiprotein complexes. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Immunocytochemical study of BCR and bcr-abl localization in K562 cells Article published earlier |
| spellingShingle | Immunocytochemical study of BCR and bcr-abl localization in K562 cells Telegeev, G.D. Dubrovska, A.N. Nadgorna, V.A. Dybkov, M.V. Zavelevich, M.P. Maliuta, S.S. Gluzman, D.F. Original contributions |
| title | Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_full | Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_fullStr | Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_full_unstemmed | Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_short | Immunocytochemical study of BCR and bcr-abl localization in K562 cells |
| title_sort | immunocytochemical study of bcr and bcr-abl localization in k562 cells |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138603 |
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