In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1
Long-term survival of patients with hepatocellular carcinoma (HCC), a common cancer worldwide, remains poor, due to metastasis and recurrence. Aim: To investigate the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HCC cell line Sk-He...
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| Published in: | Experimental Oncology |
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| Date: | 2010 |
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| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2010
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/138604 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 / M.W. Roomi, N.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 84-91. — Бібліогр.: 25 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862542613244018688 |
|---|---|
| author | Roomi, M.W. Roomi, N.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. |
| author_facet | Roomi, M.W. Roomi, N.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. |
| citation_txt | In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 / M.W. Roomi, N.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 84-91. — Бібліогр.: 25 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Long-term survival of patients with hepatocellular carcinoma (HCC), a common cancer worldwide, remains poor, due to metastasis and recurrence. Aim: To investigate the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HCC cell line Sk-Hep-1 In vivo and In vitro. Methods: After one week of isolation, 5–6 week old male athymic nude mice were inoculated with 3 x 106 SK-Hep-1 cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM In vitro on SK-Hep-1 cells, measuring cell proliferation by MTT assay, invasion through Matrigel, apoptosis by green caspase detection kit, MMP secretion by zymography, and morphology by H&E staining. Results: NM inhibited tumor weight and burden of SK-Hep-1 xenografts by 42% and 33% respectively. In vitro, NM exhibited 33% toxicity over the control at 500 and 1000 μg/ml concentration. Zymography demonstrated MMP-2 and MMP-9 secretion which was inhibited by NM in a dose dependent fashion, with virtual total inhibition at 1000 μg/ml. Invasion through Matrigel was inhibited at 100, 500 and 1000 μg/ml by 53%, 83% and 100% respectively. NM induced slight apoptosis at 100 μg/ml, and profound apoptosis at 500 μg/ml and 1000 μg/ml concentration. Conclusions: These results suggest that NM has therapeutic potential in treatment of HCC.
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| first_indexed | 2025-11-24T20:28:34Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-138604 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-24T20:28:34Z |
| publishDate | 2010 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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| spelling | Roomi, M.W. Roomi, N.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. 2018-06-19T10:24:28Z 2018-06-19T10:24:28Z 2010 In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 / M.W. Roomi, N.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2010. — Т. 32, № 2. — С. 84-91. — Бібліогр.: 25 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138604 Long-term survival of patients with hepatocellular carcinoma (HCC), a common cancer worldwide, remains poor, due to metastasis and recurrence. Aim: To investigate the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HCC cell line Sk-Hep-1 In vivo and In vitro. Methods: After one week of isolation, 5–6 week old male athymic nude mice were inoculated with 3 x 106 SK-Hep-1 cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM In vitro on SK-Hep-1 cells, measuring cell proliferation by MTT assay, invasion through Matrigel, apoptosis by green caspase detection kit, MMP secretion by zymography, and morphology by H&E staining. Results: NM inhibited tumor weight and burden of SK-Hep-1 xenografts by 42% and 33% respectively. In vitro, NM exhibited 33% toxicity over the control at 500 and 1000 μg/ml concentration. Zymography demonstrated MMP-2 and MMP-9 secretion which was inhibited by NM in a dose dependent fashion, with virtual total inhibition at 1000 μg/ml. Invasion through Matrigel was inhibited at 100, 500 and 1000 μg/ml by 53%, 83% and 100% respectively. NM induced slight apoptosis at 100 μg/ml, and profound apoptosis at 500 μg/ml and 1000 μg/ml concentration. Conclusions: These results suggest that NM has therapeutic potential in treatment of HCC. The research study was funded by Dr. Rath Health Foundation (Plantation, Florida, USA) a non-profit organization. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 Article published earlier |
| spellingShingle | In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 Roomi, M.W. Roomi, N.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. Original contributions |
| title | In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_full | In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_fullStr | In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_full_unstemmed | In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_short | In vivo and In vitro effect of a nutrient mixture on human hepatocarcinoma cell line SK-HEP-1 |
| title_sort | in vivo and in vitro effect of a nutrient mixture on human hepatocarcinoma cell line sk-hep-1 |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138604 |
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