Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients

Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients

Background: The complete medical consequences of the long-term exposure of population to ionizing radiation in post-Chernobyl period are still a controversial issue. The molecular biological analysis of malignant diseases of hematopoietic and lymphoid tissues in contaminated territories requires the...

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Published in:Experimental Oncology
Date:2011
Main Authors: Savli, H., Gluzman, D.F., Sunnetci, D., Zavelevich, M.P., Sklyarenko, L.M., Nadgornaya, V.A., Koval, S.V.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2011
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/138622
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients / H. Savli, D.F. Gluzman, D. Sunnetci, M.P. Zavelevich, L.M. Sklyarenko, V.A. Nadgornaya, S.V. Koval // Experimental Oncology. — 2011. — Т. 33, № 2. — С. 104-106. — Бібліогр.: 19 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138622
record_format dspace
spelling Savli, H.
Gluzman, D.F.
Sunnetci, D.
Zavelevich, M.P.
Sklyarenko, L.M.
Nadgornaya, V.A.
Koval, S.V.
2018-06-19T10:41:46Z
2018-06-19T10:41:46Z
2011
Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients / H. Savli, D.F. Gluzman, D. Sunnetci, M.P. Zavelevich, L.M. Sklyarenko, V.A. Nadgornaya, S.V. Koval // Experimental Oncology. — 2011. — Т. 33, № 2. — С. 104-106. — Бібліогр.: 19 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138622
Background: The complete medical consequences of the long-term exposure of population to ionizing radiation in post-Chernobyl period are still a controversial issue. The molecular biological analysis of malignant diseases of hematopoietic and lymphoid tissues in contaminated territories requires the precise diagnosis based on criteria of novel classifications. Aim: To analyze the relative gene expression of six apoptosis-related genes in different types of tumors of hematopoietic and lymphoid tissues in patients living in areas of Ukraine contaminated with radionuclides in post-Chernobyl period. Material and methods: The samples of the peripheral blood and bone marrow of 189 Ukrainian leukemia patients and 16 patients with reactive lymphocytosis were analyzed morphologically and immunocytochemically for precise delineation of the main forms and cytological variants of hematological malignancies according to new WHO classification. Expression of six apoptosis-related genes was analyzed in the individual samples of 9 different groups of malignant diseases of hematopoietic and lymphoid tissues and one group of patients with reactive lymphocytosis by quantitative RT-PCR. Expression of genes was assessed relative to that in control group of healthy donors. Results: Up-regulation of six analyzed apoptosis-related genes is observed in all groups of leukemia. In most groups of leukemia being analyzed, BCL-2 up-regulation level is superior to that of BAX. Prominent MYC up-regulation is observed in B-lymphoblastic leukemia/lymphoma, non-Hodgkin’s lymphoma, and T-lymphoblastic leukemia/lymphoma groups. In myelodysplastic/myeloproliferative neoplasms, the striking up-regulation of Fas-1 and P38MAPK is evident. Practically all the groups of leukemia are characterized by stable high ratios of P53 up-regulation. Conclusion: In Ukrainian patients, up-regulation of six analyzed apoptosis-related genes is observed practically in all types of malignant diseases of hematopoietic and lymphoid tissues under study. Microarray-based analysis of these samples would be of great importance in terms of elucidating genomic interactions in leukemias and their possible association with ionizing radiation.
The study was financed within the framework of the joint research project M/32–2008 “Cytomorphological, immunocytochemical and molecular biological features of leukemias in persons exposed to ionizing radiation” according to the Agreement between the Ministry of Education and Science of Ukraine and the Scientific and Technical Research Council of Turkey (TUBITAK).
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients
spellingShingle Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients
Savli, H.
Gluzman, D.F.
Sunnetci, D.
Zavelevich, M.P.
Sklyarenko, L.M.
Nadgornaya, V.A.
Koval, S.V.
Original contributions
title_short Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients
title_full Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients
title_fullStr Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients
title_full_unstemmed Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients
title_sort quantitative real time pcr analysis of apoptosis-related gene expression in leukemias in ukrainian patients
author Savli, H.
Gluzman, D.F.
Sunnetci, D.
Zavelevich, M.P.
Sklyarenko, L.M.
Nadgornaya, V.A.
Koval, S.V.
author_facet Savli, H.
Gluzman, D.F.
Sunnetci, D.
Zavelevich, M.P.
Sklyarenko, L.M.
Nadgornaya, V.A.
Koval, S.V.
topic Original contributions
topic_facet Original contributions
publishDate 2011
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Background: The complete medical consequences of the long-term exposure of population to ionizing radiation in post-Chernobyl period are still a controversial issue. The molecular biological analysis of malignant diseases of hematopoietic and lymphoid tissues in contaminated territories requires the precise diagnosis based on criteria of novel classifications. Aim: To analyze the relative gene expression of six apoptosis-related genes in different types of tumors of hematopoietic and lymphoid tissues in patients living in areas of Ukraine contaminated with radionuclides in post-Chernobyl period. Material and methods: The samples of the peripheral blood and bone marrow of 189 Ukrainian leukemia patients and 16 patients with reactive lymphocytosis were analyzed morphologically and immunocytochemically for precise delineation of the main forms and cytological variants of hematological malignancies according to new WHO classification. Expression of six apoptosis-related genes was analyzed in the individual samples of 9 different groups of malignant diseases of hematopoietic and lymphoid tissues and one group of patients with reactive lymphocytosis by quantitative RT-PCR. Expression of genes was assessed relative to that in control group of healthy donors. Results: Up-regulation of six analyzed apoptosis-related genes is observed in all groups of leukemia. In most groups of leukemia being analyzed, BCL-2 up-regulation level is superior to that of BAX. Prominent MYC up-regulation is observed in B-lymphoblastic leukemia/lymphoma, non-Hodgkin’s lymphoma, and T-lymphoblastic leukemia/lymphoma groups. In myelodysplastic/myeloproliferative neoplasms, the striking up-regulation of Fas-1 and P38MAPK is evident. Practically all the groups of leukemia are characterized by stable high ratios of P53 up-regulation. Conclusion: In Ukrainian patients, up-regulation of six analyzed apoptosis-related genes is observed practically in all types of malignant diseases of hematopoietic and lymphoid tissues under study. Microarray-based analysis of these samples would be of great importance in terms of elucidating genomic interactions in leukemias and their possible association with ionizing radiation.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138622
citation_txt Quantitative real time PCR analysis of apoptosis-related gene expression in leukemias in ukrainian patients / H. Savli, D.F. Gluzman, D. Sunnetci, M.P. Zavelevich, L.M. Sklyarenko, V.A. Nadgornaya, S.V. Koval // Experimental Oncology. — 2011. — Т. 33, № 2. — С. 104-106. — Бібліогр.: 19 назв. — англ.
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first_indexed 2025-12-07T16:55:05Z
last_indexed 2025-12-07T16:55:05Z
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