Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice

Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice

Aim: Our main objective was to determine the effect of ascorbate supplementation in mice unable to synthesize ascorbic acid (gulo KO) when challenged with murine B16FO cancer cells. Methods: Gulo KO female mice 36–40 weeks of age were deprived of or maintained on ascorbate in food and water for 4 we...

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Veröffentlicht in:Experimental Oncology
Datum:2011
Hauptverfasser: Cha, J., Roomi, M.W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A., Rath, M.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2011
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/138671
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Zitieren:Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice / J. Cha, M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 226-230. — Бібліогр.: 20 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138671
record_format dspace
spelling Cha, J.
Roomi, M.W.
Ivanov, V.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
2018-06-19T11:23:44Z
2018-06-19T11:23:44Z
2011
Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice / J. Cha, M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 226-230. — Бібліогр.: 20 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138671
Aim: Our main objective was to determine the effect of ascorbate supplementation in mice unable to synthesize ascorbic acid (gulo KO) when challenged with murine B16FO cancer cells. Methods: Gulo KO female mice 36–40 weeks of age were deprived of or maintained on ascorbate in food and water for 4 weeks prior to subcutaneous injection of 2.5×106 B16FO murine melanoma cells in the right flank of mice. A control group of wild type mice were also injected with the melanoma cells and maintained on a regular murine diet. Mice were continued on their respective diets for another 2 weeks after injection. The mice were then sacrificed, blood was drawn and their tumors were measured, excised and processed for histology. Results: Mean weight of animals decreased significantly (30%, p < 0.0001) in the ascorbate-restricted group but increased slightly, but insignificantly, in the ascorbate-supplemented group. The mean tumor weight in ascorbate supplemented mice was significantly reduced (by 64%, p = 0.004) compared to tumor weight in ascorbate-deprived gulo mice. The mean tumor weight of wild type mice did not differ significantly from the ascorbate-supplemented mice. Gulo KO mice supplemented with ascorbate developed smaller tumors with more collagen encapsulation and fibrous capsule interdigitation, while gulo KO mice deprived of ascorbate hosted large tumors with poorly defined borders, showing more necrosis and mitosis. Ascorbate supplementation of gulo KO mice resulted in profoundly decreased serum inflammatory cytokine IL-6 (90% decrease, p = 0.04) and IL-1β (62% decrease) compared to the levels in gulo KO mice deprived of ascorbate. Conclusion: Ascorbate supplementation modulated tumor growth and inflammatory cytokine secretion as well as enhanced encapsulation of tumors in scorbutic mice.
Special thanks to Earl Rainey for animal colony maintenance. The research study was funded by Dr. Rath Health Foundation (Santa Clara, CA, USA), a non-profit organization.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
spellingShingle Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
Cha, J.
Roomi, M.W.
Ivanov, V.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
Original contributions
title_short Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
title_full Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
title_fullStr Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
title_full_unstemmed Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice
title_sort ascorbate depletion increases growth and metastasis of melanoma cells in vitamin c deficient mice
author Cha, J.
Roomi, M.W.
Ivanov, V.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
author_facet Cha, J.
Roomi, M.W.
Ivanov, V.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
topic Original contributions
topic_facet Original contributions
publishDate 2011
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: Our main objective was to determine the effect of ascorbate supplementation in mice unable to synthesize ascorbic acid (gulo KO) when challenged with murine B16FO cancer cells. Methods: Gulo KO female mice 36–40 weeks of age were deprived of or maintained on ascorbate in food and water for 4 weeks prior to subcutaneous injection of 2.5×106 B16FO murine melanoma cells in the right flank of mice. A control group of wild type mice were also injected with the melanoma cells and maintained on a regular murine diet. Mice were continued on their respective diets for another 2 weeks after injection. The mice were then sacrificed, blood was drawn and their tumors were measured, excised and processed for histology. Results: Mean weight of animals decreased significantly (30%, p < 0.0001) in the ascorbate-restricted group but increased slightly, but insignificantly, in the ascorbate-supplemented group. The mean tumor weight in ascorbate supplemented mice was significantly reduced (by 64%, p = 0.004) compared to tumor weight in ascorbate-deprived gulo mice. The mean tumor weight of wild type mice did not differ significantly from the ascorbate-supplemented mice. Gulo KO mice supplemented with ascorbate developed smaller tumors with more collagen encapsulation and fibrous capsule interdigitation, while gulo KO mice deprived of ascorbate hosted large tumors with poorly defined borders, showing more necrosis and mitosis. Ascorbate supplementation of gulo KO mice resulted in profoundly decreased serum inflammatory cytokine IL-6 (90% decrease, p = 0.04) and IL-1β (62% decrease) compared to the levels in gulo KO mice deprived of ascorbate. Conclusion: Ascorbate supplementation modulated tumor growth and inflammatory cytokine secretion as well as enhanced encapsulation of tumors in scorbutic mice.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138671
citation_txt Ascorbate depletion increases growth and metastasis of melanoma cells in vitamin C deficient mice / J. Cha, M.W. Roomi, V. Ivanov, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 226-230. — Бібліогр.: 20 назв. — англ.
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