Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system

Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system

Aim: To study the differential expression of PKD1 and PKD2 in primary gastric cancer samples and to examine the role of PKD1 and PKD2 protein kinases in regulation of gastric tumor cell biology in the model system. Methods: Tumor samples of different histological variants of primary gastric cancer w...

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Veröffentlicht in:Experimental Oncology
Datum:2011
Hauptverfasser: Shabelnik, M.Yu., Kovalevska, L.M., Yurchenko, M.Y., Shlapatska, L.M., Rzepetsky, Yu., Sidorenko, S.P.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2011
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/138681
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Zitieren:Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system / M.Yu. Shabelnik, L.M. Kovalevska, M.Y. Yurchenko, L.M. Shlapatska, Yu. Rzepetsky, S.P. Sidorenko // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 206-211. — Бібліогр.: 20 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138681
record_format dspace
spelling Shabelnik, M.Yu.
Kovalevska, L.M.
Yurchenko, M.Y.
Shlapatska, L.M.
Rzepetsky, Yu.
Sidorenko, S.P.
2018-06-19T11:30:33Z
2018-06-19T11:30:33Z
2011
Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system / M.Yu. Shabelnik, L.M. Kovalevska, M.Y. Yurchenko, L.M. Shlapatska, Yu. Rzepetsky, S.P. Sidorenko // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 206-211. — Бібліогр.: 20 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138681
Aim: To study the differential expression of PKD1 and PKD2 in primary gastric cancer samples and to examine the role of PKD1 and PKD2 protein kinases in regulation of gastric tumor cell biology in the model system. Methods: Tumor samples of different histological variants of primary gastric cancer were analyzed. PKD1 and PKD2 expression levels in tumor samples were accessed by Western blot analysis and quantitative polymerase chain reaction (Q-PCR). As a model system we have used gastric adenocarcinoma сell line AGS sublines constitutively transfected by pcDNA3.1 coding PKD1 or PKD2, or empty pcDNA3.1 vector. These cell lines were analyzed by Western blot, Q-PCR, MTT and proliferation assays, in vitro scratch and Transwell assays, clonogenic assay. Results: It was found that primary gastric tumors possess different levels of PKD1 and PKD2 expression on mRNA and protein levels. Low level of PKD1 expression on protein and mRNA level was detected in low differentiated adenocarcinoma and ring cell gastric cancer — disorders with poor clinical prognosis. The high level of PKD2 expression was also found in gastric tumors with poor prognosis: low differentiated adenocarcinoma and adenogen cancer. To find out whether differential expression of PKD1 and PKD2 could affect biology of gastric tumor cells in vitro, we used a model system based on AGS cell line that constitutively expressed PKD1 or overexpressed PKD2. PKD1 transfection led to the inhibition of cell proliferation, migration and colony formation, in the meanwhile, the PKD2 overexpression enhanced proliferation, migration and colony formation capacities of AGS cells. Conclusions: Our data suggest that both downregulation of PKD1 or upregulation of PKD2 expression may determine the behavior of gastric tumor cells, which promotes invasive phenotype and could result in general poor prognosis.
This study has been supported by the Ukrainian Governmental Grants: 0110U005757 and 0110U006647. We thank to Prof. J. Van Lint (Belgium) and Prof. T. Seufferlein (Germany) for kind gifts of PKD1 and PKD2 constructs.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system
spellingShingle Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system
Shabelnik, M.Yu.
Kovalevska, L.M.
Yurchenko, M.Y.
Shlapatska, L.M.
Rzepetsky, Yu.
Sidorenko, S.P.
Original contributions
title_short Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system
title_full Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system
title_fullStr Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system
title_full_unstemmed Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system
title_sort differential expression of pkd1 and pkd2 in gastric cancer and analysis of pkd1 and pkd2 function in the model system
author Shabelnik, M.Yu.
Kovalevska, L.M.
Yurchenko, M.Y.
Shlapatska, L.M.
Rzepetsky, Yu.
Sidorenko, S.P.
author_facet Shabelnik, M.Yu.
Kovalevska, L.M.
Yurchenko, M.Y.
Shlapatska, L.M.
Rzepetsky, Yu.
Sidorenko, S.P.
topic Original contributions
topic_facet Original contributions
publishDate 2011
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To study the differential expression of PKD1 and PKD2 in primary gastric cancer samples and to examine the role of PKD1 and PKD2 protein kinases in regulation of gastric tumor cell biology in the model system. Methods: Tumor samples of different histological variants of primary gastric cancer were analyzed. PKD1 and PKD2 expression levels in tumor samples were accessed by Western blot analysis and quantitative polymerase chain reaction (Q-PCR). As a model system we have used gastric adenocarcinoma сell line AGS sublines constitutively transfected by pcDNA3.1 coding PKD1 or PKD2, or empty pcDNA3.1 vector. These cell lines were analyzed by Western blot, Q-PCR, MTT and proliferation assays, in vitro scratch and Transwell assays, clonogenic assay. Results: It was found that primary gastric tumors possess different levels of PKD1 and PKD2 expression on mRNA and protein levels. Low level of PKD1 expression on protein and mRNA level was detected in low differentiated adenocarcinoma and ring cell gastric cancer — disorders with poor clinical prognosis. The high level of PKD2 expression was also found in gastric tumors with poor prognosis: low differentiated adenocarcinoma and adenogen cancer. To find out whether differential expression of PKD1 and PKD2 could affect biology of gastric tumor cells in vitro, we used a model system based on AGS cell line that constitutively expressed PKD1 or overexpressed PKD2. PKD1 transfection led to the inhibition of cell proliferation, migration and colony formation, in the meanwhile, the PKD2 overexpression enhanced proliferation, migration and colony formation capacities of AGS cells. Conclusions: Our data suggest that both downregulation of PKD1 or upregulation of PKD2 expression may determine the behavior of gastric tumor cells, which promotes invasive phenotype and could result in general poor prognosis.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138681
citation_txt Differential expression of PKD1 and PKD2 in gastric cancer and analysis of PKD1 and PKD2 function in the model system / M.Yu. Shabelnik, L.M. Kovalevska, M.Y. Yurchenko, L.M. Shlapatska, Yu. Rzepetsky, S.P. Sidorenko // Experimental Oncology. — 2011. — Т. 33, № 4. — С. 206-211. — Бібліогр.: 20 назв. — англ.
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first_indexed 2025-12-02T07:00:57Z
last_indexed 2025-12-02T07:00:57Z
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