Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by lase...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2012 |
| Hauptverfasser: | , , , , , , , |
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| Sprache: | Englisch |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2012
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| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/138708 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo / L.S. Rieznichenko, S.M. Dybkova, T.G. Gruzina, Z.R. Ulberg, I.N. Todor, N.Yu. Lukyanova, S.I. Shpyleva, V.F. Chekhun // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 25-28. — Бібліогр.: 34 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862732347109015552 |
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| author | Rieznichenko, L.S. Dybkova, S.M. Gruzina, T.G. Ulberg, Z.R. Todor, I.N. Lukyanova, N.Yu. Shpyleva, S.I. Chekhun, V.F. |
| author_facet | Rieznichenko, L.S. Dybkova, S.M. Gruzina, T.G. Ulberg, Z.R. Todor, I.N. Lukyanova, N.Yu. Shpyleva, S.I. Chekhun, V.F. |
| citation_txt | Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo / L.S. Rieznichenko, S.M. Dybkova, T.G. Gruzina, Z.R. Ulberg, I.N. Todor, N.Yu. Lukyanova, S.I. Shpyleva, V.F. Chekhun // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 25-28. — Бібліогр.: 34 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by laser-correlation spectroscopy and transmission electron microscopy methods. The GNP interaction with tumor cells has been visualized by confocal microscopy method. The enzyme activity was determined by standard biochemical methods. GNP distribution and content in organs and tissues have been determined via atomic-absorption spectrometry method; genotoxic influence has been estimated by “Comet-assay” method. Results: The GNP size-dependent accumulation in cultured U937 tumor cells and their ability to modulate U937 cell membrane Na+,K+-АТР-ase activity value has been revealed in vitro. Using in vivo model of Guerin carcinoma it has been shown that GNP possess high affinity to tumor cells. Conclusions: Our results indicate the perspectives of use of the synthesized GNP water dispersions for cancer diagnostics and treatment. It’s necessary to take into account a size-dependent biosafety level of nanoparticles.
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| first_indexed | 2025-12-07T19:31:07Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-138708 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T19:31:07Z |
| publishDate | 2012 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Rieznichenko, L.S. Dybkova, S.M. Gruzina, T.G. Ulberg, Z.R. Todor, I.N. Lukyanova, N.Yu. Shpyleva, S.I. Chekhun, V.F. 2018-06-19T11:57:16Z 2018-06-19T11:57:16Z 2012 Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo / L.S. Rieznichenko, S.M. Dybkova, T.G. Gruzina, Z.R. Ulberg, I.N. Todor, N.Yu. Lukyanova, S.I. Shpyleva, V.F. Chekhun // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 25-28. — Бібліогр.: 34 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138708 The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by laser-correlation spectroscopy and transmission electron microscopy methods. The GNP interaction with tumor cells has been visualized by confocal microscopy method. The enzyme activity was determined by standard biochemical methods. GNP distribution and content in organs and tissues have been determined via atomic-absorption spectrometry method; genotoxic influence has been estimated by “Comet-assay” method. Results: The GNP size-dependent accumulation in cultured U937 tumor cells and their ability to modulate U937 cell membrane Na+,K+-АТР-ase activity value has been revealed in vitro. Using in vivo model of Guerin carcinoma it has been shown that GNP possess high affinity to tumor cells. Conclusions: Our results indicate the perspectives of use of the synthesized GNP water dispersions for cancer diagnostics and treatment. It’s necessary to take into account a size-dependent biosafety level of nanoparticles. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo Article published earlier |
| spellingShingle | Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo Rieznichenko, L.S. Dybkova, S.M. Gruzina, T.G. Ulberg, Z.R. Todor, I.N. Lukyanova, N.Yu. Shpyleva, S.I. Chekhun, V.F. Original contributions |
| title | Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo |
| title_full | Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo |
| title_fullStr | Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo |
| title_full_unstemmed | Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo |
| title_short | Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo |
| title_sort | gold nanoparticles synthesis and biological activity estimation in vitro and in vivo |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138708 |
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