Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo

Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo

The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by lase...

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Veröffentlicht in:Experimental Oncology
Datum:2012
Hauptverfasser: Rieznichenko, L.S., Dybkova, S.M., Gruzina, T.G., Ulberg, Z.R., Todor, I.N., Lukyanova, N.Yu., Shpyleva, S.I., Chekhun, V.F.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2012
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/138708
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Zitieren:Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo / L.S. Rieznichenko, S.M. Dybkova, T.G. Gruzina, Z.R. Ulberg, I.N. Todor, N.Yu. Lukyanova, S.I. Shpyleva, V.F. Chekhun // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 25-28. — Бібліогр.: 34 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138708
record_format dspace
spelling Rieznichenko, L.S.
Dybkova, S.M.
Gruzina, T.G.
Ulberg, Z.R.
Todor, I.N.
Lukyanova, N.Yu.
Shpyleva, S.I.
Chekhun, V.F.
2018-06-19T11:57:16Z
2018-06-19T11:57:16Z
2012
Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo / L.S. Rieznichenko, S.M. Dybkova, T.G. Gruzina, Z.R. Ulberg, I.N. Todor, N.Yu. Lukyanova, S.I. Shpyleva, V.F. Chekhun // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 25-28. — Бібліогр.: 34 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138708
The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by laser-correlation spectroscopy and transmission electron microscopy methods. The GNP interaction with tumor cells has been visualized by confocal microscopy method. The enzyme activity was determined by standard biochemical methods. GNP distribution and content in organs and tissues have been determined via atomic-absorption spectrometry method; genotoxic influence has been estimated by “Comet-assay” method. Results: The GNP size-dependent accumulation in cultured U937 tumor cells and their ability to modulate U937 cell membrane Na+,K+-АТР-ase activity value has been revealed in vitro. Using in vivo model of Guerin carcinoma it has been shown that GNP possess high affinity to tumor cells. Conclusions: Our results indicate the perspectives of use of the synthesized GNP water dispersions for cancer diagnostics and treatment. It’s necessary to take into account a size-dependent biosafety level of nanoparticles.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
spellingShingle Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
Rieznichenko, L.S.
Dybkova, S.M.
Gruzina, T.G.
Ulberg, Z.R.
Todor, I.N.
Lukyanova, N.Yu.
Shpyleva, S.I.
Chekhun, V.F.
Original contributions
title_short Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
title_full Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
title_fullStr Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
title_full_unstemmed Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
title_sort gold nanoparticles synthesis and biological activity estimation in vitro and in vivo
author Rieznichenko, L.S.
Dybkova, S.M.
Gruzina, T.G.
Ulberg, Z.R.
Todor, I.N.
Lukyanova, N.Yu.
Shpyleva, S.I.
Chekhun, V.F.
author_facet Rieznichenko, L.S.
Dybkova, S.M.
Gruzina, T.G.
Ulberg, Z.R.
Todor, I.N.
Lukyanova, N.Yu.
Shpyleva, S.I.
Chekhun, V.F.
topic Original contributions
topic_facet Original contributions
publishDate 2012
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description The aim of the work was the synthesis of gold nanoparticles (GNP) of different sizes and the estimation of their biological activity in vitro and in vivo. Materials and Methods: Water dispersions of gold nanoparticles of different sizes have been synthesized by Davis method and characterized by laser-correlation spectroscopy and transmission electron microscopy methods. The GNP interaction with tumor cells has been visualized by confocal microscopy method. The enzyme activity was determined by standard biochemical methods. GNP distribution and content in organs and tissues have been determined via atomic-absorption spectrometry method; genotoxic influence has been estimated by “Comet-assay” method. Results: The GNP size-dependent accumulation in cultured U937 tumor cells and their ability to modulate U937 cell membrane Na+,K+-АТР-ase activity value has been revealed in vitro. Using in vivo model of Guerin carcinoma it has been shown that GNP possess high affinity to tumor cells. Conclusions: Our results indicate the perspectives of use of the synthesized GNP water dispersions for cancer diagnostics and treatment. It’s necessary to take into account a size-dependent biosafety level of nanoparticles.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138708
citation_txt Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo / L.S. Rieznichenko, S.M. Dybkova, T.G. Gruzina, Z.R. Ulberg, I.N. Todor, N.Yu. Lukyanova, S.I. Shpyleva, V.F. Chekhun // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 25-28. — Бібліогр.: 34 назв. — англ.
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first_indexed 2025-12-07T19:31:07Z
last_indexed 2025-12-07T19:31:07Z
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