Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model

Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model

Aim: Experimental and clinical studies showed that the administration of cyclophosphamide (Cy) in low doses leads to an enhancement of the antitumor immune response. Our objective was to study the modulation, if any, by low dose Cy, of T regulatory (Treg) and natural killer T (NKT) I cells, two cell...

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Published in:Experimental Oncology
Date:2012
Main Authors: Rico, M.J., Rozados, V.R., Mainetti, L.E., Zacarias Fluck, M.F., Matar, P., Scharovsky, O.G.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2012
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/138719
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model / M.J. Rico, V.R. Rozados, L.E. Mainetti, M.F. Zacarias Fluck, P. Matar, O.G. Scharovsky // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 38-42. — Бібліогр.: 36 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138719
record_format dspace
spelling Rico, M.J.
Rozados, V.R.
Mainetti, L.E.
Zacarias Fluck, M.F.
Matar, P.
Scharovsky, O.G.
2018-06-19T12:11:05Z
2018-06-19T12:11:05Z
2012
Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model / M.J. Rico, V.R. Rozados, L.E. Mainetti, M.F. Zacarias Fluck, P. Matar, O.G. Scharovsky // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 38-42. — Бібліогр.: 36 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138719
Aim: Experimental and clinical studies showed that the administration of cyclophosphamide (Cy) in low doses leads to an enhancement of the antitumor immune response. Our objective was to study the modulation, if any, by low dose Cy, of T regulatory (Treg) and natural killer T (NKT) I cells, two cell populations of the innate immune response with opposing effects on the tumors, in a rat B cell lymphoma model. Methods: Inbred e rats were challenged s.c. with L-TACB lymphoma and on day 14 animals were distributed in two groups. Treated: injected i.p. with cyclophosphamide (10mg/kg of body weight) and Control: injected i.p. with saline. Blood samples were taken from days 0 to 21 and the percentage of T regulatory and natural killer T I cells were determined by flow cytometry. Results: We found that the increase of natural and inducible T regulatory cells of peripheral blood achieved during tumor growth was significantly downregulated by cyclophosphamide. On the contrary, natural killer T I cells were not modulated by the treatment. Conclusion: The antimetastatic effect of a single low dose of Cy would be due, at least in part, to downregulation of natural and inducible T regulatory cells.
We would like to thank Cibic S.A. and especially to Dr. Ricardo Giordano, for their help in flow cytometry studies. This work was supported by a grant from Universidad Nacional de Rosario to O.G. Scharovsky.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model
spellingShingle Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model
Rico, M.J.
Rozados, V.R.
Mainetti, L.E.
Zacarias Fluck, M.F.
Matar, P.
Scharovsky, O.G.
Original contributions
title_short Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model
title_full Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model
title_fullStr Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model
title_full_unstemmed Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model
title_sort regulatory t cells but not nkt i cells are modulated by a single low-dose cyclophosphamide in a b cell lymphoma tumor-model
author Rico, M.J.
Rozados, V.R.
Mainetti, L.E.
Zacarias Fluck, M.F.
Matar, P.
Scharovsky, O.G.
author_facet Rico, M.J.
Rozados, V.R.
Mainetti, L.E.
Zacarias Fluck, M.F.
Matar, P.
Scharovsky, O.G.
topic Original contributions
topic_facet Original contributions
publishDate 2012
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: Experimental and clinical studies showed that the administration of cyclophosphamide (Cy) in low doses leads to an enhancement of the antitumor immune response. Our objective was to study the modulation, if any, by low dose Cy, of T regulatory (Treg) and natural killer T (NKT) I cells, two cell populations of the innate immune response with opposing effects on the tumors, in a rat B cell lymphoma model. Methods: Inbred e rats were challenged s.c. with L-TACB lymphoma and on day 14 animals were distributed in two groups. Treated: injected i.p. with cyclophosphamide (10mg/kg of body weight) and Control: injected i.p. with saline. Blood samples were taken from days 0 to 21 and the percentage of T regulatory and natural killer T I cells were determined by flow cytometry. Results: We found that the increase of natural and inducible T regulatory cells of peripheral blood achieved during tumor growth was significantly downregulated by cyclophosphamide. On the contrary, natural killer T I cells were not modulated by the treatment. Conclusion: The antimetastatic effect of a single low dose of Cy would be due, at least in part, to downregulation of natural and inducible T regulatory cells.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138719
citation_txt Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model / M.J. Rico, V.R. Rozados, L.E. Mainetti, M.F. Zacarias Fluck, P. Matar, O.G. Scharovsky // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 38-42. — Бібліогр.: 36 назв. — англ.
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first_indexed 2025-12-07T17:10:43Z
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