Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model
Aim: Experimental and clinical studies showed that the administration of cyclophosphamide (Cy) in low doses leads to an enhancement of the antitumor immune response. Our objective was to study the modulation, if any, by low dose Cy, of T regulatory (Treg) and natural killer T (NKT) I cells, two cell...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2012 |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2012
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model / M.J. Rico, V.R. Rozados, L.E. Mainetti, M.F. Zacarias Fluck, P. Matar, O.G. Scharovsky // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 38-42. — Бібліогр.: 36 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862708253184491520 |
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| author | Rico, M.J. Rozados, V.R. Mainetti, L.E. Zacarias Fluck, M.F. Matar, P. Scharovsky, O.G. |
| author_facet | Rico, M.J. Rozados, V.R. Mainetti, L.E. Zacarias Fluck, M.F. Matar, P. Scharovsky, O.G. |
| citation_txt | Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model / M.J. Rico, V.R. Rozados, L.E. Mainetti, M.F. Zacarias Fluck, P. Matar, O.G. Scharovsky // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 38-42. — Бібліогр.: 36 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: Experimental and clinical studies showed that the administration of cyclophosphamide (Cy) in low doses leads to an enhancement of the antitumor immune response. Our objective was to study the modulation, if any, by low dose Cy, of T regulatory (Treg) and natural killer T (NKT) I cells, two cell populations of the innate immune response with opposing effects on the tumors, in a rat B cell lymphoma model. Methods: Inbred e rats were challenged s.c. with L-TACB lymphoma and on day 14 animals were distributed in two groups. Treated: injected i.p. with cyclophosphamide (10mg/kg of body weight) and Control: injected i.p. with saline. Blood samples were taken from days 0 to 21 and the percentage of T regulatory and natural killer T I cells were determined by flow cytometry. Results: We found that the increase of natural and inducible T regulatory cells of peripheral blood achieved during tumor growth was significantly downregulated by cyclophosphamide. On the contrary, natural killer T I cells were not modulated by the treatment. Conclusion: The antimetastatic effect of a single low dose of Cy would be due, at least in part, to downregulation of natural and inducible T regulatory cells.
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| first_indexed | 2025-12-07T17:10:43Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-138719 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T17:10:43Z |
| publishDate | 2012 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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| spelling | Rico, M.J. Rozados, V.R. Mainetti, L.E. Zacarias Fluck, M.F. Matar, P. Scharovsky, O.G. 2018-06-19T12:11:05Z 2018-06-19T12:11:05Z 2012 Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model / M.J. Rico, V.R. Rozados, L.E. Mainetti, M.F. Zacarias Fluck, P. Matar, O.G. Scharovsky // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 38-42. — Бібліогр.: 36 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138719 Aim: Experimental and clinical studies showed that the administration of cyclophosphamide (Cy) in low doses leads to an enhancement of the antitumor immune response. Our objective was to study the modulation, if any, by low dose Cy, of T regulatory (Treg) and natural killer T (NKT) I cells, two cell populations of the innate immune response with opposing effects on the tumors, in a rat B cell lymphoma model. Methods: Inbred e rats were challenged s.c. with L-TACB lymphoma and on day 14 animals were distributed in two groups. Treated: injected i.p. with cyclophosphamide (10mg/kg of body weight) and Control: injected i.p. with saline. Blood samples were taken from days 0 to 21 and the percentage of T regulatory and natural killer T I cells were determined by flow cytometry. Results: We found that the increase of natural and inducible T regulatory cells of peripheral blood achieved during tumor growth was significantly downregulated by cyclophosphamide. On the contrary, natural killer T I cells were not modulated by the treatment. Conclusion: The antimetastatic effect of a single low dose of Cy would be due, at least in part, to downregulation of natural and inducible T regulatory cells. We would like to thank Cibic S.A. and especially to Dr. Ricardo Giordano, for their help in flow cytometry studies. This work was supported by a grant from Universidad Nacional de Rosario to O.G. Scharovsky. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model Article published earlier |
| spellingShingle | Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model Rico, M.J. Rozados, V.R. Mainetti, L.E. Zacarias Fluck, M.F. Matar, P. Scharovsky, O.G. Original contributions |
| title | Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model |
| title_full | Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model |
| title_fullStr | Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model |
| title_full_unstemmed | Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model |
| title_short | Regulatory T cells but not NKT I cells are modulated by a single low-dose Cyclophosphamide in a B cell lymphoma tumor-model |
| title_sort | regulatory t cells but not nkt i cells are modulated by a single low-dose cyclophosphamide in a b cell lymphoma tumor-model |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138719 |
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