The long-range cytotoxic effect in tumor-bearing animals
Aim:The relationship between cancer and patient health is still of great interest for experimental and clinical oncology. The tumor can adversely affect surrounding and distant tissues as well. However, effects of the tumor on distant tissues are much less studied than its effects on surrounding tis...
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| Опубліковано в: : | Experimental Oncology |
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| Дата: | 2012 |
| Автори: | , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2012
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| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/138721 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | The long-range cytotoxic effect in tumor-bearing animals / B.I. Gerashchenko, N.M. Ryabchenko, O.A. Glavin, V.M. Mikhailenko // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 34-37. — Бібліогр.: 21 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862729069414580224 |
|---|---|
| author | Gerashchenko, B.I. Ryabchenko, N.M. Glavin, O.A. Mikhailenko, V.M. |
| author_facet | Gerashchenko, B.I. Ryabchenko, N.M. Glavin, O.A. Mikhailenko, V.M. |
| citation_txt | The long-range cytotoxic effect in tumor-bearing animals / B.I. Gerashchenko, N.M. Ryabchenko, O.A. Glavin, V.M. Mikhailenko // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 34-37. — Бібліогр.: 21 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim:The relationship between cancer and patient health is still of great interest for experimental and clinical oncology. The tumor can adversely affect surrounding and distant tissues as well. However, effects of the tumor on distant tissues are much less studied than its effects on surrounding tissues. This study was aimed to test whether the tumor could trigger cytotoxic and/or genotoxic signals with respect to the distant proliferative tissue such as bone marrow. Materials and Methods: Rats were subcutaneously implanted with Guerin carcinoma cells, and on the 12th and 18th days after implantation both cytotoxic and genotoxic effects were assessed by flow cytometry in acridine orange stained unfractionated bone marrow cells isolated from femur. The cytotoxic effect was assessed using ratios of the following cell populations: total nucleated cells (TNC)/total enucleated erythrocytes (TE); polychromatic erythrocytes (PCE)/normochromatic erythrocytes (NCE). The genotoxic effect was assessed by quantification of micronucleated PCE (MNPCE) within the population of PCE. Results: A significant cytotoxic effect was observed in tumor-bearing animals on the 12th and 18th days after implantation (≈ 2-fold decrease in both TNC/TE and PCE/NCE ratios compared with corresponding parameters in control animals). There was also a genotoxic effect in these animals (a slight increase in the number of MNPCE), however, this effect was insignificant. The PCE/NCE ratio reversely correlated with the tumor weight which is suggestive of the link between erythropoietic cytotoxicity and tumor progression. Conclusion: Cytotoxic insult to the bone marrow is likely to be associated with the mechanism(s) triggered by distantly located tumors whose growth may correlate with the cytotoxic effect.
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| first_indexed | 2025-12-07T19:12:17Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-138721 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T19:12:17Z |
| publishDate | 2012 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Gerashchenko, B.I. Ryabchenko, N.M. Glavin, O.A. Mikhailenko, V.M. 2018-06-19T12:11:54Z 2018-06-19T12:11:54Z 2012 The long-range cytotoxic effect in tumor-bearing animals / B.I. Gerashchenko, N.M. Ryabchenko, O.A. Glavin, V.M. Mikhailenko // Experimental Oncology. — 2012. — Т. 34, № 1. — С. 34-37. — Бібліогр.: 21 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138721 Aim:The relationship between cancer and patient health is still of great interest for experimental and clinical oncology. The tumor can adversely affect surrounding and distant tissues as well. However, effects of the tumor on distant tissues are much less studied than its effects on surrounding tissues. This study was aimed to test whether the tumor could trigger cytotoxic and/or genotoxic signals with respect to the distant proliferative tissue such as bone marrow. Materials and Methods: Rats were subcutaneously implanted with Guerin carcinoma cells, and on the 12th and 18th days after implantation both cytotoxic and genotoxic effects were assessed by flow cytometry in acridine orange stained unfractionated bone marrow cells isolated from femur. The cytotoxic effect was assessed using ratios of the following cell populations: total nucleated cells (TNC)/total enucleated erythrocytes (TE); polychromatic erythrocytes (PCE)/normochromatic erythrocytes (NCE). The genotoxic effect was assessed by quantification of micronucleated PCE (MNPCE) within the population of PCE. Results: A significant cytotoxic effect was observed in tumor-bearing animals on the 12th and 18th days after implantation (≈ 2-fold decrease in both TNC/TE and PCE/NCE ratios compared with corresponding parameters in control animals). There was also a genotoxic effect in these animals (a slight increase in the number of MNPCE), however, this effect was insignificant. The PCE/NCE ratio reversely correlated with the tumor weight which is suggestive of the link between erythropoietic cytotoxicity and tumor progression. Conclusion: Cytotoxic insult to the bone marrow is likely to be associated with the mechanism(s) triggered by distantly located tumors whose growth may correlate with the cytotoxic effect. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions The long-range cytotoxic effect in tumor-bearing animals Article published earlier |
| spellingShingle | The long-range cytotoxic effect in tumor-bearing animals Gerashchenko, B.I. Ryabchenko, N.M. Glavin, O.A. Mikhailenko, V.M. Original contributions |
| title | The long-range cytotoxic effect in tumor-bearing animals |
| title_full | The long-range cytotoxic effect in tumor-bearing animals |
| title_fullStr | The long-range cytotoxic effect in tumor-bearing animals |
| title_full_unstemmed | The long-range cytotoxic effect in tumor-bearing animals |
| title_short | The long-range cytotoxic effect in tumor-bearing animals |
| title_sort | long-range cytotoxic effect in tumor-bearing animals |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138721 |
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