Cytometry of apoptosis. Historical perspective and new advances

Characteristic changes in cell morphology paralleled by the appearance of a multitude of molecular and biochemical markers occur during apoptosis. These changes vary depending on the cell type, mechanism of induction of apoptosis, and the time-window at which the process of apoptosis is analyzed. By...

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Опубліковано в: :Experimental Oncology
Дата:2012
Автори: Wlodkowic, D., Skommer, J., Darzynkiewicz, Z.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2012
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Онлайн доступ:https://nasplib.isofts.kiev.ua/handle/123456789/139051
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Cytometry of apoptosis. Historical perspective and new advances / D. Wlodkovic, J. Skommer, Z. Darzynkiewicz // Experimental Oncology. — 2012. — Т. 34, № 3. — С. 255-262. — Бібліогр.: 78 назв. — англ.

Репозитарії

Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-139051
record_format dspace
spelling Wlodkowic, D.
Skommer, J.
Darzynkiewicz, Z.
2018-06-19T18:48:05Z
2018-06-19T18:48:05Z
2012
Cytometry of apoptosis. Historical perspective and new advances / D. Wlodkovic, J. Skommer, Z. Darzynkiewicz // Experimental Oncology. — 2012. — Т. 34, № 3. — С. 255-262. — Бібліогр.: 78 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/139051
Characteristic changes in cell morphology paralleled by the appearance of a multitude of molecular and biochemical markers occur during apoptosis. These changes vary depending on the cell type, mechanism of induction of apoptosis, and the time-window at which the process of apoptosis is analyzed. By virtue of the capability of rapid measurement of individual cells the flow- and imaging-cytometry become preferred technologies to detect, identify and record incidence of apoptosis in large cell populations. It also provided a valuable tool to investigate molecular mechanisms in field of necrobiology. This review outlines the progress in development of the most commonly used cytometric methods probing cells death based on analysis of fragmentation of DNA, activation of caspases, analysis of mitochondrial potential, alterations in plasma membrane structure and other features that characterize programmed cell death. This article is part of a Special Issue entitled “Apoptosis: Four Decades Later”
Supported by NIH NCI RO1 28 704 and by the Robert Welke Foundation for Cancer Research (ZD); Faculty Research Development Fund, University of Auckland (DW); Early Career Research Excellence Award, University of Auckland (DW); NZ Foundation for Research Science and Technology (DW); NZ Neurological Foundation (JS).
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Reviews
Cytometry of apoptosis. Historical perspective and new advances
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Cytometry of apoptosis. Historical perspective and new advances
spellingShingle Cytometry of apoptosis. Historical perspective and new advances
Wlodkowic, D.
Skommer, J.
Darzynkiewicz, Z.
Reviews
title_short Cytometry of apoptosis. Historical perspective and new advances
title_full Cytometry of apoptosis. Historical perspective and new advances
title_fullStr Cytometry of apoptosis. Historical perspective and new advances
title_full_unstemmed Cytometry of apoptosis. Historical perspective and new advances
title_sort cytometry of apoptosis. historical perspective and new advances
author Wlodkowic, D.
Skommer, J.
Darzynkiewicz, Z.
author_facet Wlodkowic, D.
Skommer, J.
Darzynkiewicz, Z.
topic Reviews
topic_facet Reviews
publishDate 2012
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Characteristic changes in cell morphology paralleled by the appearance of a multitude of molecular and biochemical markers occur during apoptosis. These changes vary depending on the cell type, mechanism of induction of apoptosis, and the time-window at which the process of apoptosis is analyzed. By virtue of the capability of rapid measurement of individual cells the flow- and imaging-cytometry become preferred technologies to detect, identify and record incidence of apoptosis in large cell populations. It also provided a valuable tool to investigate molecular mechanisms in field of necrobiology. This review outlines the progress in development of the most commonly used cytometric methods probing cells death based on analysis of fragmentation of DNA, activation of caspases, analysis of mitochondrial potential, alterations in plasma membrane structure and other features that characterize programmed cell death. This article is part of a Special Issue entitled “Apoptosis: Four Decades Later”
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/139051
citation_txt Cytometry of apoptosis. Historical perspective and new advances / D. Wlodkovic, J. Skommer, Z. Darzynkiewicz // Experimental Oncology. — 2012. — Т. 34, № 3. — С. 255-262. — Бібліогр.: 78 назв. — англ.
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first_indexed 2025-11-28T13:51:02Z
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