Cytometry of apoptosis. Historical perspective and new advances

Characteristic changes in cell morphology paralleled by the appearance of a multitude of molecular and biochemical markers occur during apoptosis. These changes vary depending on the cell type, mechanism of induction of apoptosis, and the time-window at which the process of apoptosis is analyzed. By...

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Veröffentlicht in:Experimental Oncology
Datum:2012
Hauptverfasser: Wlodkowic, D., Skommer, J., Darzynkiewicz, Z.
Format: Artikel
Sprache:Englisch
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2012
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/139051
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Zitieren:Cytometry of apoptosis. Historical perspective and new advances / D. Wlodkovic, J. Skommer, Z. Darzynkiewicz // Experimental Oncology. — 2012. — Т. 34, № 3. — С. 255-262. — Бібліогр.: 78 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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author Wlodkowic, D.
Skommer, J.
Darzynkiewicz, Z.
author_facet Wlodkowic, D.
Skommer, J.
Darzynkiewicz, Z.
citation_txt Cytometry of apoptosis. Historical perspective and new advances / D. Wlodkovic, J. Skommer, Z. Darzynkiewicz // Experimental Oncology. — 2012. — Т. 34, № 3. — С. 255-262. — Бібліогр.: 78 назв. — англ.
collection DSpace DC
container_title Experimental Oncology
description Characteristic changes in cell morphology paralleled by the appearance of a multitude of molecular and biochemical markers occur during apoptosis. These changes vary depending on the cell type, mechanism of induction of apoptosis, and the time-window at which the process of apoptosis is analyzed. By virtue of the capability of rapid measurement of individual cells the flow- and imaging-cytometry become preferred technologies to detect, identify and record incidence of apoptosis in large cell populations. It also provided a valuable tool to investigate molecular mechanisms in field of necrobiology. This review outlines the progress in development of the most commonly used cytometric methods probing cells death based on analysis of fragmentation of DNA, activation of caspases, analysis of mitochondrial potential, alterations in plasma membrane structure and other features that characterize programmed cell death. This article is part of a Special Issue entitled “Apoptosis: Four Decades Later”
first_indexed 2025-11-28T13:51:02Z
format Article
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institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
issn 1812-9269
language English
last_indexed 2025-11-28T13:51:02Z
publishDate 2012
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
record_format dspace
spelling Wlodkowic, D.
Skommer, J.
Darzynkiewicz, Z.
2018-06-19T18:48:05Z
2018-06-19T18:48:05Z
2012
Cytometry of apoptosis. Historical perspective and new advances / D. Wlodkovic, J. Skommer, Z. Darzynkiewicz // Experimental Oncology. — 2012. — Т. 34, № 3. — С. 255-262. — Бібліогр.: 78 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/139051
Characteristic changes in cell morphology paralleled by the appearance of a multitude of molecular and biochemical markers occur during apoptosis. These changes vary depending on the cell type, mechanism of induction of apoptosis, and the time-window at which the process of apoptosis is analyzed. By virtue of the capability of rapid measurement of individual cells the flow- and imaging-cytometry become preferred technologies to detect, identify and record incidence of apoptosis in large cell populations. It also provided a valuable tool to investigate molecular mechanisms in field of necrobiology. This review outlines the progress in development of the most commonly used cytometric methods probing cells death based on analysis of fragmentation of DNA, activation of caspases, analysis of mitochondrial potential, alterations in plasma membrane structure and other features that characterize programmed cell death. This article is part of a Special Issue entitled “Apoptosis: Four Decades Later”
Supported by NIH NCI RO1 28 704 and by the
 Robert Welke Foundation for Cancer Research (ZD);
 Faculty Research Development Fund, University
 of Auckland (DW); Early Career Research Excellence
 Award, University of Auckland (DW); NZ Foundation
 for Research Science and Technology (DW); NZ Neurological
 Foundation (JS).
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Reviews
Cytometry of apoptosis. Historical perspective and new advances
Article
published earlier
spellingShingle Cytometry of apoptosis. Historical perspective and new advances
Wlodkowic, D.
Skommer, J.
Darzynkiewicz, Z.
Reviews
title Cytometry of apoptosis. Historical perspective and new advances
title_full Cytometry of apoptosis. Historical perspective and new advances
title_fullStr Cytometry of apoptosis. Historical perspective and new advances
title_full_unstemmed Cytometry of apoptosis. Historical perspective and new advances
title_short Cytometry of apoptosis. Historical perspective and new advances
title_sort cytometry of apoptosis. historical perspective and new advances
topic Reviews
topic_facet Reviews
url https://nasplib.isofts.kiev.ua/handle/123456789/139051
work_keys_str_mv AT wlodkowicd cytometryofapoptosishistoricalperspectiveandnewadvances
AT skommerj cytometryofapoptosishistoricalperspectiveandnewadvances
AT darzynkiewiczz cytometryofapoptosishistoricalperspectiveandnewadvances