Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations
Aim: On the basis of the cytogenetic research, to develop and validate the strategy of the measures to prevent the stochastic effects of low-doses radiation on humans. Methods: Test system with human peripheral blood lymphocytes, metaphase analysis of chromosomal aberrations was used. Cells were cul...
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2013
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| Cite this: | Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations / E.A. Domina, V.F. Chekhun // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 65-68. — Бібліогр.: 27 назв. — англ. |
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| author | Domina, E.A. Chekhun, V.F. |
| author_facet | Domina, E.A. Chekhun, V.F. |
| citation_txt | Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations / E.A. Domina, V.F. Chekhun // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 65-68. — Бібліогр.: 27 назв. — англ. |
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| description | Aim: On the basis of the cytogenetic research, to develop and validate the strategy of the measures to prevent the stochastic effects of low-doses radiation on humans. Methods: Test system with human peripheral blood lymphocytes, metaphase analysis of chromosomal aberrations was used. Cells were cultured according to the standard procedures with modifications. The analysis of painted chromosome preparations was carried out according to the conventional requirements to metaphase spread. Results: The experimental material, obtained on chromosomal level of radiosensitive cells, concerning validation of prevention strategy of stochastic effects of low doses of ionizing radiation, primarily cancer, is discussed. Its key phases are the following: estimation of individual radiosensitivity, accounting of the co-mutagens influence and use of effective atoxic radioprotectors. The practicability of the primary prevention strategy of radiogenic cancer has been evidence based, especially in case of the influence of small doses of ionizing radiation. Cytogenetic studies using G2-radiation sensitivity assay are essential component of priority populations’ health monitoring for formation high cancer risk groups and implementation developed strategies of stochastic effects prevention, including radiogenic cancer, among persons with known hypersensitivity to ionizing radiation. It applies the nuclear industry workers, medical staff (radiation oncologists, radiologists), and priority populations living in areas contaminated with radionuclides. Conclusion: Strategy for the prevention of stochastic effects of low-doses radiation, especially cancer risk, is elaborated on the cytogenetic studies basis, implies that cancer risk reduction is provided by assessment of individual radiation sensitivity (G2-radiation sensitivity assay), by taking into account the additional effect of co-mutagens, and with the use of non-toxic effective radioprotectors.
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Experimental Oncology ��� ������ ���� ��arc����� ������ ���� ��arc�� ��arc�� ��
EXPERIMENTAL VALIDATION OF PREVENTION
OF THE DEVELOPMENT OF STOCHASTIC EFFECTS OF LOW DOSES
OF IONIZING RADIATION BASED ON THE ANALYSIS OF HUMAN
LYMPHOCYTES’ CHROMOSOME ABERRATIONS
E.A. Domina, V.F. Chekhun
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine
Aim: On the basis of the cytogenetic research, to develop and validate the strategy of the measures to prevent the stochastic effects
of low-doses radiation on humans. Methods: Test system with human peripheral blood lymphocytes, metaphase analysis of chromo-
somal aberrations was used. Cells were cultured according to the standard procedures with modifications. The analysis of painted
chromosome preparations was carried out according to the conventional requirements to metaphase spread. Results: The experimen-
tal material, obtained on chromosomal level of radiosensitive cells, concerning validation of prevention strategy of stochastic effects
of low doses of ionizing radiation, primarily cancer, is discussed. Its key phases are the following: estimation of individual radiosensi-
tivity, accounting of the co-mutagens influence and use of effective atoxic radioprotectors. The practicability of the primary prevention
strategy of radiogenic cancer has been evidence based, especially in case of the influence of small doses of ionizing radiation. Cytoge-
netic studies using G2-radiation sensitivity assay are essential component of priority populations’ health monitoring for formation high
cancer risk groups and implementation developed strategies of stochastic effects prevention, including radiogenic cancer, among
persons with known hypersensitivity to ionizing radiation. It applies the nuclear industry workers, medical staff (radiation oncologists,
radiologists), and priority populations living in areas contaminated with radionuclides. Conclusion: Strategy for the prevention of sto-
chastic effects of low-doses radiation, especially cancer risk, is elaborated on the cytogenetic studies basis, implies that cancer risk
reduction is provided by assessment of individual radiation sensitivity (G2-radiation sensiti vity assay), by taking into account the ad-
ditional effect of co-mutagens, and with the use of non-toxic effective radioprotectors.
Key Words: prevention, stochastic effects, radiation sensitivity, cytogenetic effect, co-mutagens, radioprotectors.
T�e stoc�astic effects of low doses of ionizing radia-
tion also include c�romosome aberrations and cancer�
t�e incidence of w�ic� is a probabilistic process and does
not �ave dose t�res�old [�]. It was found t�at t�e effect
of irradiation on t�e process of carcinogene sis at low
doses may be greater per dose unit t�an larger doses.
Researc�ers attribute t�is to less expression of apoptosis�
reparation� c�ange of t�e irradiated cells sensitivity to t�e
action of ot�er carcinogenic factors in comparison wit� t�e
effects of large doses of radiation� reduction of t�e organ-
ism’s compensatory and recovery capabilities� etc. [���].
T�e problem of radiogenic cancer �as become espe-
cially actual and �as obtained global scale in connection
wit� t�e accident in C�ernobyl Nuclear Power Plant �April
�9��� and Fukusima-�� Japan ��arc� ������ w�at �as
pointed out t�at nuclear reactors �ave no absolute guar-
antee of safe operation.
To date t�ere is no t�eory on �ow to predict t�e deve-
lopment of stoc�astic effects� including radiation-induced
cancer� and c�oose t�e means of its prevention. In most
academic oncological centers of CIS t�e researc� para-
digm is focused on creation and improvement of curative
met�ods� on t�e problem and investigation of cancero-
genesis� on early detection of diseases. However� not
enoug� attention �as been paid to primary prevention
of cancer� including t�e one of radiation genesis.
Using t�e modern knowledge about t�e role of t�e
genetic factor in t�e development of oncologic diseases
is a potentially fruitful area of individual prevention of t�is
disease. T�e in�erited genetic susceptibility� genomic
instability� mutation modifications in proto-oncogenes
and suppressor genes� dynamic researc� of c�romo-
somal aberration level and spectrum etc.� are t�e key
genetic factors indicating to �ig� carcinogenic risk.
T�e results of t�e researc�es of genetic susceptibility
to irradiation effect s�ow sufficiently �ig� risk of deter-
ministic and stoc�astic effects development� w�ic� t�e
individuals wit� radiosensitive genotype �ave� especially
in case of low radiation influence. T�is is crucial in t�e
case of medical examination of employees of compa-
nies wit� �ig� carcinogenic risk conditions.
By now it �as been accumulated t�e data� w�ic�
s�ow t�e relations�ip of mutagenesis in somatic cells
wit� carcinogenesis� and c�romosomal aberrations are
sensitive indicator of radiation exposure on t�e �uman
organism. T�is allows t�e use of cytogenetic indicators
as prognostic markers of t�e oncological pat�ology risk
development [�].
According to current views� t�e radiation-induced
destabilization of �uman genome is potentially onco-
genic [7]; and �uman perip�eral blood lymp�ocytes
�HPBL� �T-lymp�ocytes� are unique researc� object
wit� special c�aracteristics being an object of radiation
and cytogenetic researc� [�]. T�e basic premise for t�is
is a �ig� c�romosomal radiosensitivity of lymp�ocytes
comparing to t�e c�romosomes of ot�er cells as in vivo
as in vitro� w�ic� allows us to register indubitable
increase of induced c�romosomal aberrations level
Received: February 10, 2012.
*Correspondence: E-mail: edjomina@ukr.net
Abbreviations used: HPBL — human peripheral blood lymphocytes;
IRS — individual radiation sensitivity; RAR — radioadaptive response.
Exp Oncol ����
��� �� �����
�� Experimental Oncology ��� ������ ���� ��arc��
compared wit� spontaneous one� in low levels of irradia-
tion. A �ig� mobility of lymp�ocytes in blood stream� t�e
distribution of lymp� nodes all over t�e body� t�e ability
of lymp�ocytes to accumulate c�romosome damages
not only make it possible to draw conclusions about
t�e radiosensitivity of �uman organism as a w�ole� but
to prognosticate t�e consequences of irradiation. In t�e
radiation cytogenetics guidelines t�ere are numerous
statements about approximately equal outlet of c�ro-
mosomal aberrations in case of lymp�ocytes irradiation
in vivo and in vitro; t�at means t�at t�e cells respond
to t�e irradiation as autonomic biological system [�� 9].
Taking into account t�e importance of t�e prob-
lem of negative biomedical effects of small exposure
doses� t�is study presents a strategy of t�eir preven-
tion� w�ic� is argued by cytogenetic studies data. T�e
proposed prevention strategy includes t�e following
key stages: estimation of individual radiosensitivity�
accounting of t�e co-mutagens influence and use
of effective atoxic radioprotectors.
MATERIALS AND METHODS
We used test system wit� HPBL� metap�ase analy-
sis of c�romosomal aberrations. T�e protocol for study
was approved by local Et�ics Committee.
Analysis of aberrations level and spectrum in c�ro-
mosomes of HPBL� w�ic� are acknowledged to be one
of t�e most sensitive to radiation and are recommended
WHO and UNSCEAR for biological indication of t�e radia-
tion injury of �uman organism [��]� gives an objective in-
formation about genome integrity in �uman somatic cells.
Lymphocytes cultures. Cells were cultured according
to t�e standard procedures wit� modifications [��]. Cells
were incubated in RP�I ��4� medium� containing �.� μg/
ml PHA �� form� Gibco-Invitrogen� for �� � �last � � wit�
colcemid�. T�is procedure made it possible to analyze cells
in t�e first post-radiation mitosis. T�e analysis of painted
c�romosome preparations was carried out according to t�e
conventional requirements to metap�ase spread [��].
Statistical analysis. Cytogenetic parameters
obtained were analyzed by t�e means of standard de-
scriptive and variation statistics and included calculation
of mean group values ���� standard error �SE�� stan-
dard deviation �SD�� sample dispersion �s��� coefficient
of variation �CV� etc. and representing experimental data
distributions as �istograms. After analysis of t�eir forms
and fitting of obtained functions to normal ones 9�%
confidential intervals were determined as �±� 9�SD. Ra-
diosensitive cut-off point was also calculated as t�e 9�t�
percentile of obtained G� scores. F-test was applied to in-
dicate significance of t�e differences between donors.
A significance level of p < �.�� was used t�roug�out.
RESULTS AND DISCUSSION
Assessment of human organism individual radia-
tion sensitivity (IRS) w�ic� makes it possible to prog-
nosticate t�e risk for pat�ological radiogenic condition.
According to t�e modern views� cells sensitivity to t�e
influence of ionizing irradiation is formed by a complex
of factors: on t�e one �and� t�e particularities of genetic
structure and conformation of DNA� t�e level of endo-
genous protectors� antioxidant activity� c�aracteristics
of cell cycle� intensity of apoptosis� regulation of prolifera-
tion processes� effectiveness of reparation system etc.;
on t�e ot�er �and� t�e level of integral absorbed radia-
tion dose and its distribution in time and space� terms
after irradiation� as well as t�e c�aracter of influence
combination wit� ot�er environmental factors. In case
of assessment of general �uman radiation sensitivity t�e
individual differences are neutralized. However� in case
of equal dose of irradiation t�e large amplitude of IRS
values means t�at �ig� variability is observed. In case
of �ig� stress intensity �uman individual c�aracteristics
do not play a crucial role� as t�e damage exceeds pro-
tective and compensative abilities of t�e organism. T�e
modern point of view on etiology of radiogenic cancer
is a dominant carcinogenic danger of t�e influence of low
doses of irradiation. �oreover� most of radiation effects
of tec�nogenic sources are c�aracterized by low doses
and low power of doses. T�at’s w�y t�e definition of IRS
is especially important in t�e range of influence of low
doses of ionizing radiation [�����]. From t�is point
of view it is recommended to use G�-radiation sensitivity
assay� w�ic� we designed on t�e basis of classical t�eses
of radiation cytogenetics� in order to identify individuals
wit� �ig� IRS in a �ealt�y co�ort [��].
According to t�e developed algorit�m �Figure��
t�e IRS determination of relatively �ealt�y individuals
is advisable to carry out under t�e following conditions:
• testing γ-irradiation of HPBL cultures s�ould
be done in t�e most radiosensitive period of t�e first
mitotic cycle — late G� �4� � of cells incubation�;
• dose of γ-irradiation is �.� Gy at power �.� Gy/min�
w�ic� allows to identify t�e maximum variability
of t�e IRS indicators;
• cell culture fixation for �� � of incubation takes
into account radiation-induced mitotic delay
and provides metap�ase analysis of aberrations
of c�romatid type �deletions�� t�at are dominant
in t�e injury spectrum of G�-period �Fig. ��.
For practical use of cytogenetic G�-factor test it was
proposed t�e coefficient of IRS �CIRS�� w�ic� is t�e ratio of:
CIRS = MIRS/М� w�ere
MIRS — t�e total frequency of c�romosomal aber-
rations in individual HPBL;
M — t�e range of normal values for t�is indicator
�� ± ��9� σ�.
If for �ypersensitive persons t�e radiation-induced
cytogenetic effect wit� t�e same radiation dose ex-
ceeds t�e upper bound of t�e range of normal values
variability� t�e ratio is always > �� w�ereas in �yposensi-
tive persons it will be < �.
T�is met�od allows to estimate genetically deter-
mined sensitivity of t�e individual to t�e radiation factor.
As c�romosomal modifications development in cell
population is considered to be potentially oncogenic
[�7]� t�en t�e increase of radiation sensitivity comparing
to its average population values is a risk factor of radia-
tion carcinogenesis. In t�is connection we �ave deve-
loped t�e indications for cytogenetic examination of t�e
individuals w�o are working �or w�o are going to work�
Experimental Oncology ��� ������ ���� ��arc����� ������ ���� ��arc�� ��arc�� �7
in t�e range of action of ionizing radiation� as well as for
ot�er priority categories of t�e population.
Figure. Algorit�m of individual radiosensitivity detection in prac-
tically �ealt�y individuals by cytogenetics alterations in peri-
p�eral blood lymp�ocytes culture. FHA — p�yto�emagglutinin;
R — gamma-irradiation in dose equal to �.� Gy; K — colcemid;
F — terms of cell culture fixation
Accounting of the co-mutagens influence.
T�ese are t�e substances w�ic�� not �aving own in-
trinsic mutagenic properties� can considerably modify
�intensify� t�e effects of well-known mutagens� includ-
ing radiation-induced effects of low doses. Particular
danger in t�is case present suc� medicines as calcium
antagonists �verapamil� intensifies bleomycine action��
ascorbic acid �intensifies t�e actions of �ydrogen
�ydroperoxide or bleomycine�� caffeine �increases
cytogenetic effects of met�otrexate� etc. In spite of t�e
fact t�at t�ere are some data s�owing t�e possibility
of co-mutagen modification of induced mutagenesis�
t�is problem as a w�ole is not given appropriate con-
sideration. T�e issue on possible role of t�e regulation
of reparation processes in co-mutagen effects forma-
tion in �uman cells still remains open� but t�eoretically
it can be related to t�e in�ibition of t�ese processes
[��]. We �ave s�own t�at suc� co-mutagen as caffeine
�≥ ��� μg/ml of blood� �.7-fold increases t�e radiation-
induced cytogenetic effect� wit�out influencing spon-
taneous level of c�romosome aberration in �uman
somatic radiosensitive cells. T�is effect is due to t�e
aberrations of c�romosomal type� predominantly paired
fragments and dicentric c�romosomes [�9]. Presented
data indicate t�at individuals wit� determined increased
IRS s�ould be encouraged to limit consumption of sub-
stances wit� known co-mutagen effect.
Use of effective atoxic radioprotectors. T�ese
are t�e substances given prior to irradiation for reduc-
tion of its impact on organism and for �uman genome
resistance intensification. Examples of suc� radiopro-
tectors are inosine and t�ymalin� t�eir action is due
to activation of enzymatic reparation processes.
Inosine� substance of nature origin� being precursor
of ATP and nucleotides synt�esis� maintains energy ba-
lance in cells of different tissues� possesses anti�ypoxic
properties� and stimulates reparation as well as diffe-
rent metabolic processes. We �ave s�own t�at inosine
reduces t�e level of c�romosome aberrations induced
in LPB of �ealt�y individuals in t�e range of low-doses
γ-irradiation in vitro to t�e values of spontaneous aber-
rations� so playing t�e role of radioprotector for cells. T�e
most pronounced effect of inosine in t�e preventive dose
�estimated at �.�� mg/ml of blood� is observed at t�e
lowest irradiation doses �.� — �.� — �.� Gy �Table�.
Table. Modification of radiation-induced cytogenetic effects in lympho-
cytes of peripheral blood of healthy individuals under the inosine influence
(mean group values)
Dose,
Gy
Cytogenetic indicators (per 100 metaphases analyzed)
Frequency
of aberrant
metaphas-
es, %
Total frequen-
cy of chromo-
some aberra-
tions, %
Aberration
of chro-
mosomal
type, %
Dicen-
trics,
%
Aberrations
of chroma-
tide type, %
Dele-
tions,
%
0.1 6.06 ± 0.6 6.06 ± 0.6 2.99 0.3 3.07 3.0
In + 0.1 1.3 ± 0.1 1.66 ± 0.1 1.06 0.2 0.6 0.6
0.2 7.0 ± 1.6 7.06 ± 1.6 3.26 0.5 3.8 3.6
In + 0,2 2.6 ± 0.4 2.7 ± 0.4 1.6 0.5 1.1 0.75
0.3 7.5 ± 1.0 7.76 ± 1.0 4.16 0.9 3.6 3.4
In + 0.3 2.2 ± 0.6 2.2 ± 0.6 1.2 0.2 1.0 0.8
0.5 10.9 ± 1.4 11.3 ± 1.4 5.23 1.3 5.9 5.6
In + 0.5 3.5 ± 1.0 4.5 ± 1.0 3.5 1.5 1.0 0.5
1.0 17.4 ± 1.5 18.6 ± 1.8 11.4 5.4 7.2 6.8
In + 1.0 14.8 ± 1.1 15.3 ± 1.0 8.6 4.2 6.7 6.0
Note: In — inosine.
In t�is dose range t�e level of radiation-induced
c�romosome aberrations reduces� reac�ing values
of average population level of spontaneous genetic
alterations in HPBL. T�e coefficient of modification
of radiation effects is �.� �± �.�� — �.7 �± �.�� —
�.� �± �.��� respectively. Wit� furt�er dose increase
to �.� Gy radioprotective effect of inosine reduces� and
coefficient of modification is� respectively� �.� [��].
W�ile searc�ing means capable to restore cells
from radiation-induced c�anges� it was found t�at
agents of t�ymus origin� including t�ymalin� may be ef-
fective for t�ese purposes� as targets for t�eir action
are just �uman lymp�ocytes.
T�ymalin is a complex mixture of biologically ac-
tive substances� mainly peptides� isolated from t�e
mammals t�ymus tissues. It refers to medicines t�at
increase genome stability� activate immune and repair
systems. T�ymalin in prop�ylactic dose �estimated
at �.��� mg/ml of blood� �as radioprotective effect
on t�e genetic apparatus of HPBL. At a dose of �.� Gy �is
effect is reduction of c�romosomal aberrations incidence
from �.� ± �.�% to �.� ± �.9%� and at a dose of �.� Gy —
from �.� ± �.�% to 4.� ± �.�%� i.e. twice. In t�e low-dose
range under t�ymalin impact t�e ray markers — dicentric
c�romosomes — disappeared. T�e observed radiopro-
tective effect of t�ymalin is due to its stimulating effect
on t�e repair of primary radiation damages in t�e first
period of t�e intermolecular test� t�at is� on t�e border
of t�e periods of G�/S of mitotic cycle [��].
Recommended drugs will ex�ibit radioprotective
properties in t�e best way w�en used on t�e back-
ground of vitamin supply as complementary approac�-
es to t�e protection of t�e �uman genome from t�e
mutagenic effects of low doses of ionizing radiation.
In t�e implementation of measures for radiogenic
cancer prevention� based on t�is strategy� we rec-
�� Experimental Oncology ��� ������ ���� ��arc��
ommend to take into account also information in t�e
patient’s �istory concerning precancerous conditions�
t�e set of environmental factors� lifestyle� including
adverse �ealt� �abits� unbalanced nutrition� etc.
Cytogenetic studies using G�-radiation sensitivity
assay are essential component of priority popula-
tions’ �ealt� monitoring for formation �ig� cancer
risk groups and implementation developed strategies
of stoc�astic effects prevention� including radiogenic
cancer� among persons wit� known �ypersensiti vity
to ionizing radiation [��]. Above all� it applies t�e
nuclear industry workers� medical staff �radiation on-
cologists� radiologists�� and priority populations living
in areas contaminated wit� radionuclides.
To be fair it s�ould be noted t�at researc�ers’ attention
recently is directed at finding correlations between �u-
man organism’s resistance to radiation and lymp�ocytes’
ability to generate radioresistance induced by ioni zing
radiation — radioadaptive response �RAR� [�����]. It was
s�own t�at t�e RAR formation can reduce cancer risk
at low-doses radiation [��]. However� in some studies
it was found t�at in various population groups� affected
by t�e C�ernobyl accident� t�e ability of lymp�ocytes
to form RAR is reduced or even absent [�7]. We �ave
evident data of clinical and cytogenetic survey of �7 t�ou-
sand liquidators of t�e C�ernobyl NPP accident� w�ic�
indicate t�at low-doses of ionizing radiation are statisti-
cally significant factors for t�e increased cancer risk [4].
T�erefore� we propose t�e met�od of prevention of t�e
development of stoc�astic effects of radiation is directed
at grading/minimization effect of small doses.
In conclusion� strategy for t�e prevention of stoc�as-
tic effects of low-doses radiation� especially cancer risk�
is elaborated on t�e cytogenetic studies basis� implies
t�at cancer risk reduction is provided by assessment
of IRS �G�-radiation sensitivity assay�� by taking into
account t�e additional effect of co-mutagens� and wit�
t�e use of non-toxic effective radioprotectors.
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Copyright © Experimental Oncology, 2013
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| id | nasplib_isofts_kiev_ua-123456789-139089 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-30T15:29:47Z |
| publishDate | 2013 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Domina, E.A. Chekhun, V.F. 2018-06-19T19:05:14Z 2018-06-19T19:05:14Z 2013 Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations / E.A. Domina, V.F. Chekhun // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 65-68. — Бібліогр.: 27 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/139089 Aim: On the basis of the cytogenetic research, to develop and validate the strategy of the measures to prevent the stochastic effects of low-doses radiation on humans. Methods: Test system with human peripheral blood lymphocytes, metaphase analysis of chromosomal aberrations was used. Cells were cultured according to the standard procedures with modifications. The analysis of painted chromosome preparations was carried out according to the conventional requirements to metaphase spread. Results: The experimental material, obtained on chromosomal level of radiosensitive cells, concerning validation of prevention strategy of stochastic effects of low doses of ionizing radiation, primarily cancer, is discussed. Its key phases are the following: estimation of individual radiosensitivity, accounting of the co-mutagens influence and use of effective atoxic radioprotectors. The practicability of the primary prevention strategy of radiogenic cancer has been evidence based, especially in case of the influence of small doses of ionizing radiation. Cytogenetic studies using G2-radiation sensitivity assay are essential component of priority populations’ health monitoring for formation high cancer risk groups and implementation developed strategies of stochastic effects prevention, including radiogenic cancer, among persons with known hypersensitivity to ionizing radiation. It applies the nuclear industry workers, medical staff (radiation oncologists, radiologists), and priority populations living in areas contaminated with radionuclides. Conclusion: Strategy for the prevention of stochastic effects of low-doses radiation, especially cancer risk, is elaborated on the cytogenetic studies basis, implies that cancer risk reduction is provided by assessment of individual radiation sensitivity (G2-radiation sensitivity assay), by taking into account the additional effect of co-mutagens, and with the use of non-toxic effective radioprotectors. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations Article published earlier |
| spellingShingle | Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations Domina, E.A. Chekhun, V.F. Original contributions |
| title | Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations |
| title_full | Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations |
| title_fullStr | Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations |
| title_full_unstemmed | Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations |
| title_short | Experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations |
| title_sort | experimental validation of prevention of the development of stochastic effects of low doses of ionizing radiation based on the analysis of human lymphocytes’ chromosome aberrations |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/139089 |
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