Cloning of variable fragments of tumor immunoglobulin, assembling and expressing of human SCFV protein in E. coli for anti-idiotype vaccination

• Published in: Experimental Oncology
• Date: 2013
• Main Authors: Meleshko, A.N., Vashkevich, K.P., Fomina, E.G., Scheslenok, E.P., Schkolina, T.V., Sergeev, G.V.
• Format: Article
• Language: English
• Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2013
• Subjects: Original contributions
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/139103
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Cloning of variable fragments of tumor immunoglobulin, assembling and expressing of human SCFV protein in E. coli for anti-idiotype vaccination / A.N. Meleshko, K.P. Vashkevich, E.G. Fomina, E.P. Scheslenok, T.V. Schkolina, G.V. Sergeev // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 8-14. — Бібліогр.: 34 назв. — англ.

Aim: Idiotype, the unique part of immunoglobulin molecule expressed on the surface of B-cells, represents a specific antigen for vaccination against lymphoma. We have developed a rapid method for immunoglobulin variable fragments cloning, assembling and expression of recombinant idiotype protein in...