Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines

Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines

Aim: Fanconi Anemia, an autosomal recessive disorder, is characterized by chromosomal abnormality leading to birth defects, progressive bone marrow failure, and a high probability of developing malignancy at an early age. Head and neck squamous cell carcinoma and myeloid leukemia are the major cause...

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Published in:Experimental Oncology
Date:2013
Main Authors: Roomi, M.W., Roomi, N.W., Bhanap, B., Niedzwiecki, A., Rath, M.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2013
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/139121
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines / M.W. Roomi, N.W. Roomi, B. Bhanap, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 20-24. — Бібліогр.: 10 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-139121
record_format dspace
spelling Roomi, M.W.
Roomi, N.W.
Bhanap, B.
Niedzwiecki, A.
Rath, M.
2018-06-19T19:17:13Z
2018-06-19T19:17:13Z
2013
Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines / M.W. Roomi, N.W. Roomi, B. Bhanap, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 20-24. — Бібліогр.: 10 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/139121
Aim: Fanconi Anemia, an autosomal recessive disorder, is characterized by chromosomal abnormality leading to birth defects, progressive bone marrow failure, and a high probability of developing malignancy at an early age. Head and neck squamous cell carcinoma and myeloid leukemia are the major causes of cancer related morbidity and mortality in Fanconi anemia patients. Me­thods: We investigated the effect of a nutrient mixture on Fanconi Anemia human fibroblast cell lines FA‐A:PD20 and FA‐A:PD220 on matrix metalloproteinase expression, invasion, cell proliferation, morphology and apoptosis. The cell lines were grown in a modified Dulbecco’s Eagle medium and at near confluence were treated with the nutrient mixture at increasing doses: 0; 10; 50; 100; 500; 1000 µg/ml. The cells were also treated with PMA to induce MMP-9 expression. Results: Zymography demonstrated MMP‐2 and PMA‐induced MMP‐9 activity. The nutrient mixture inhibited expression of both, MMP-2 and MMP-9, in a dose dependent manner with virtually total inhibition observed at 500 µg/ml. Matrigel invasion was inhibited in both cells lines; with 100% inhibition for FA-A:PD20 at 500 µg/ml and 100% inhibition of FA-A:P220 cells at 100 µg/ml. H&E staining did not indicate any change in cell morphology and causes apoptosis at higher doses. Conclusion: Our data demonstrated that the nutrient mixture inhibited matrix metalloproteinase expression, invasion and induced apoptosis, the important parameters for cancer prevention. The results suggest that the nutrient mixture may have therapeutic potential in Fanconi Anemia associated neoplasia.
Dr. Rath Health Foundation, a not for profit organization, provided research funding.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines
spellingShingle Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines
Roomi, M.W.
Roomi, N.W.
Bhanap, B.
Niedzwiecki, A.
Rath, M.
Original contributions
title_short Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines
title_full Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines
title_fullStr Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines
title_full_unstemmed Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines
title_sort repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines
author Roomi, M.W.
Roomi, N.W.
Bhanap, B.
Niedzwiecki, A.
Rath, M.
author_facet Roomi, M.W.
Roomi, N.W.
Bhanap, B.
Niedzwiecki, A.
Rath, M.
topic Original contributions
topic_facet Original contributions
publishDate 2013
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: Fanconi Anemia, an autosomal recessive disorder, is characterized by chromosomal abnormality leading to birth defects, progressive bone marrow failure, and a high probability of developing malignancy at an early age. Head and neck squamous cell carcinoma and myeloid leukemia are the major causes of cancer related morbidity and mortality in Fanconi anemia patients. Me­thods: We investigated the effect of a nutrient mixture on Fanconi Anemia human fibroblast cell lines FA‐A:PD20 and FA‐A:PD220 on matrix metalloproteinase expression, invasion, cell proliferation, morphology and apoptosis. The cell lines were grown in a modified Dulbecco’s Eagle medium and at near confluence were treated with the nutrient mixture at increasing doses: 0; 10; 50; 100; 500; 1000 µg/ml. The cells were also treated with PMA to induce MMP-9 expression. Results: Zymography demonstrated MMP‐2 and PMA‐induced MMP‐9 activity. The nutrient mixture inhibited expression of both, MMP-2 and MMP-9, in a dose dependent manner with virtually total inhibition observed at 500 µg/ml. Matrigel invasion was inhibited in both cells lines; with 100% inhibition for FA-A:PD20 at 500 µg/ml and 100% inhibition of FA-A:P220 cells at 100 µg/ml. H&E staining did not indicate any change in cell morphology and causes apoptosis at higher doses. Conclusion: Our data demonstrated that the nutrient mixture inhibited matrix metalloproteinase expression, invasion and induced apoptosis, the important parameters for cancer prevention. The results suggest that the nutrient mixture may have therapeutic potential in Fanconi Anemia associated neoplasia.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/139121
citation_txt Repression of matrix metalloproteinases and inhibition of cell invasion by a nutrient mixture, containing ascorbic acid, lysine, proline, and green tea extract on human fanconi anemia fibroblast cell lines / M.W. Roomi, N.W. Roomi, B. Bhanap, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 20-24. — Бібліогр.: 10 назв. — англ.
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AT roominw repressionofmatrixmetalloproteinasesandinhibitionofcellinvasionbyanutrientmixturecontainingascorbicacidlysineprolineandgreenteaextractonhumanfanconianemiafibroblastcelllines
AT bhanapb repressionofmatrixmetalloproteinasesandinhibitionofcellinvasionbyanutrientmixturecontainingascorbicacidlysineprolineandgreenteaextractonhumanfanconianemiafibroblastcelllines
AT niedzwieckia repressionofmatrixmetalloproteinasesandinhibitionofcellinvasionbyanutrientmixturecontainingascorbicacidlysineprolineandgreenteaextractonhumanfanconianemiafibroblastcelllines
AT rathm repressionofmatrixmetalloproteinasesandinhibitionofcellinvasionbyanutrientmixturecontainingascorbicacidlysineprolineandgreenteaextractonhumanfanconianemiafibroblastcelllines
first_indexed 2025-11-30T19:03:30Z
last_indexed 2025-11-30T19:03:30Z
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