Activity of thymidilate «salvage pathway» enzymes in human gastric cancer and blood serum: correlation with treatment modalities

• Published in: Experimental Oncology
• Date: 2013
• Main Authors: Borzenko, B.G., Bakurova, E.M., Popovich, Yu.A., Sidyuk, E.A., Popovich, A.Y.
• Format: Article
• Language: English
• Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2013
• Subjects: Original contributions
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/139124
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Activity of thymidilate «salvage pathway» enzymes in human gastric cancer and blood serum: correlation with treatment modalities / B.G. Borzenko, E.M. Bakurova, Yu.A. Popovich, E.A. Sidyuk, A.Y. Popovich // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 37-40. — Бібліогр.: 12 назв. — англ.

A comparative study of enzyme activity features of thymidilate “salvage pathway” synthesis in blood serum and tissues of different age patients with gastric cancer (T3–4N0-xM0) was carried out. Aim: To evaluate the diagnostic relevance of thymidilate metabolism enzymes activities and their association with tumor growth. Methods: Enzymes activities were determined by the radioisotope method and spectrophotometrically in tumor tissues and blood serum of 74 patients. Results: It was demonstrated that thymidine phosphorylase activity in gastric tumors is lower by 2.6 times as compared to non-neoplastic mucosa of resection margin. This being accompanied by decrease of its activity in the blood serum (from 47.9 ± 2.6 to 14.65 ± 2.4 nmol/min·mg, p < 0.001). An increase of thymidine kinase activity was revealed both in tumor tissues (more than 3.5 times) and in blood serum (from 3.9 ± 0,7 nmol/mg·h, to 6.8 ± 1.0 nmol/mg·h, p < 0.01). Changes in their activity in the postoperative period depended on the type of surgical procedure and tumor eradication. Conclusion: It could be suggested that control of individual dynamics of the enzymes activities in blood serum may be used as informative tool for monitoring of patients and treatment optimization.