Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice

Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice

Aim: Polychemotherapy (PCT), widely used for the antitumor treatment has a pronounced toxic effect on the organism, and its cytostatic effect sometimes is canceled by multidrug resistance of a neoplasia. Comprehension of the nature and development of pathological changes caused by the PCT during the...

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Veröffentlicht in:Experimental Oncology
Datum:2013
Hauptverfasser: Zonov, E.V., Voronina, E.I., Zenkova, M.A., Ageeva, T.A., Ryabchikova, E.I.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2013
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/139129
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Zitieren:Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice / E.V. Zonov, E.I. Voronina, M.A. Zenkova, T.A. Ageeva, E.I. Ryabchikova // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 30-36. — Бібліогр.: 30 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-139129
record_format dspace
spelling Zonov, E.V.
Voronina, E.I.
Zenkova, M.A.
Ageeva, T.A.
Ryabchikova, E.I.
2018-06-19T19:18:45Z
2018-06-19T19:18:45Z
2013
Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice / E.V. Zonov, E.I. Voronina, M.A. Zenkova, T.A. Ageeva, E.I. Ryabchikova // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 30-36. — Бібліогр.: 30 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/139129
Aim: Polychemotherapy (PCT), widely used for the antitumor treatment has a pronounced toxic effect on the organism, and its cytostatic effect sometimes is canceled by multidrug resistance of a neoplasia. Comprehension of the nature and development of pathological changes caused by the PCT during the treatment of cancer is very important to improve the efficiency of the therapy and to clarify the mechanisms of tumor-host interactions. This study was aimed to examine PCT impact on kidney cells and tissues in mice with transplanted resistant lymphosacroma (RLS) and to analyze morphology of metastases of the tumor in kidney during PCT. Materials and Methods: Male mice CBA/LacSto (55 animals) were intramuscularly implanted in the right hind paw by 105 cells/ml of tumor RLS (a diffuse large B-cell lymphosarcoma) with multi-drug resistance (MDR) phenotype. Mice received combination of cyclophosphamide (50 mg/kg), oncovin (0.1 mg/kg), hydroxydaunorubicin (4 mg/kg), and prednisone (5 mg/kg) accordingly to CHOP scheme each 7 days after inoculation of the tumor. The kidneys were sampled on days 1, 3 and 7 after each series of injection of PCT preparations and processed for light and electron microscopy, immunohistochemical analysis of Ki-67 and Apaf-1 proteins also was performed. Results: Tumor RLS produced metastases comprised of small cells in the kidneys of mice after 8 days post inoculation. Application of PCT resulted in destruction of small-cell metastases and development of many large-cell metastases in kidney. Application of PCT induced the development of prominent damage of nephron cells, primarily in S3 segments of proximal tubules. Even one series of PCT caused reduction of basal plasma folds in these cells and alteration of mitochondria. Damage of proximal tubules and involvement of distal tubules, renal bodies and interstitial tissue in the pathologic process, increased during the experiment. This work presents the description of morphological changes in kidney as well as of the tumor metastases under PCT influence. Conclusion: The obtained data should be considered while designing of remedies for recovery of internal organs functions after antitumor PCT.
This work was supported by a grant from “OPTEC” (Russia) company
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice
spellingShingle Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice
Zonov, E.V.
Voronina, E.I.
Zenkova, M.A.
Ageeva, T.A.
Ryabchikova, E.I.
Original contributions
title_short Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice
title_full Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice
title_fullStr Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice
title_full_unstemmed Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice
title_sort influence of polychemotherapy on the morphology of metastases and kidney of resistant rls-bearing mice
author Zonov, E.V.
Voronina, E.I.
Zenkova, M.A.
Ageeva, T.A.
Ryabchikova, E.I.
author_facet Zonov, E.V.
Voronina, E.I.
Zenkova, M.A.
Ageeva, T.A.
Ryabchikova, E.I.
topic Original contributions
topic_facet Original contributions
publishDate 2013
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: Polychemotherapy (PCT), widely used for the antitumor treatment has a pronounced toxic effect on the organism, and its cytostatic effect sometimes is canceled by multidrug resistance of a neoplasia. Comprehension of the nature and development of pathological changes caused by the PCT during the treatment of cancer is very important to improve the efficiency of the therapy and to clarify the mechanisms of tumor-host interactions. This study was aimed to examine PCT impact on kidney cells and tissues in mice with transplanted resistant lymphosacroma (RLS) and to analyze morphology of metastases of the tumor in kidney during PCT. Materials and Methods: Male mice CBA/LacSto (55 animals) were intramuscularly implanted in the right hind paw by 105 cells/ml of tumor RLS (a diffuse large B-cell lymphosarcoma) with multi-drug resistance (MDR) phenotype. Mice received combination of cyclophosphamide (50 mg/kg), oncovin (0.1 mg/kg), hydroxydaunorubicin (4 mg/kg), and prednisone (5 mg/kg) accordingly to CHOP scheme each 7 days after inoculation of the tumor. The kidneys were sampled on days 1, 3 and 7 after each series of injection of PCT preparations and processed for light and electron microscopy, immunohistochemical analysis of Ki-67 and Apaf-1 proteins also was performed. Results: Tumor RLS produced metastases comprised of small cells in the kidneys of mice after 8 days post inoculation. Application of PCT resulted in destruction of small-cell metastases and development of many large-cell metastases in kidney. Application of PCT induced the development of prominent damage of nephron cells, primarily in S3 segments of proximal tubules. Even one series of PCT caused reduction of basal plasma folds in these cells and alteration of mitochondria. Damage of proximal tubules and involvement of distal tubules, renal bodies and interstitial tissue in the pathologic process, increased during the experiment. This work presents the description of morphological changes in kidney as well as of the tumor metastases under PCT influence. Conclusion: The obtained data should be considered while designing of remedies for recovery of internal organs functions after antitumor PCT.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/139129
citation_txt Influence of polychemotherapy on the morphology of metastases and kidney of resistant RLS-bearing mice / E.V. Zonov, E.I. Voronina, M.A. Zenkova, T.A. Ageeva, E.I. Ryabchikova // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 30-36. — Бібліогр.: 30 назв. — англ.
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AT voroninaei influenceofpolychemotherapyonthemorphologyofmetastasesandkidneyofresistantrlsbearingmice
AT zenkovama influenceofpolychemotherapyonthemorphologyofmetastasesandkidneyofresistantrlsbearingmice
AT ageevata influenceofpolychemotherapyonthemorphologyofmetastasesandkidneyofresistantrlsbearingmice
AT ryabchikovaei influenceofpolychemotherapyonthemorphologyofmetastasesandkidneyofresistantrlsbearingmice
first_indexed 2025-12-07T19:32:50Z
last_indexed 2025-12-07T19:32:50Z
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