Search for potential gastric cancer markers using miRNA databases and gene expression analysis

Search for potential gastric cancer markers using miRNA databases and gene expression analysis

Aim: The aim of this study was to identify genes that are differentially expressed in gastric tumors and to analyze the association of their expression level with tumor clinicopathologic features. Methods: In the present research, we used bioinformatic-driven search to identify miRNA that are down-r...

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Veröffentlicht in:Experimental Oncology
Datum:2013
Hauptverfasser: Volkomorov, V., Grigoryeva, E., Krasnov, G., Litviakov, N., Tsyganov, M., Karbyshev, M., Zavyalova, M., Afanasyev, S., Cherdyntseva, N., Lisitsyn, N., Beresten, S.
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Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2013
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/139132
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Zitieren:Search for potential gastric cancer markers using miRNA databases and gene expression analysis / V. Volkomorov, E. Grigoryeva, G. Krasnov, N. Litviakov, M. Tsyganov, M. Karbyshev, M. Zavyalova, S. Afanasyev, N. Cherdyntseva, N. Lisitsyn, S. Beresten // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 2-7. — Бібліогр.: 37 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-139132
record_format dspace
spelling Volkomorov, V.
Grigoryeva, E.
Krasnov, G.
Litviakov, N.
Tsyganov, M.
Karbyshev, M.
Zavyalova, M.
Afanasyev, S.
Cherdyntseva, N.
Lisitsyn, N.
Beresten, S.
2018-06-19T19:19:27Z
2018-06-19T19:19:27Z
2013
Search for potential gastric cancer markers using miRNA databases and gene expression analysis / V. Volkomorov, E. Grigoryeva, G. Krasnov, N. Litviakov, M. Tsyganov, M. Karbyshev, M. Zavyalova, S. Afanasyev, N. Cherdyntseva, N. Lisitsyn, S. Beresten // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 2-7. — Бібліогр.: 37 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/139132
Aim: The aim of this study was to identify genes that are differentially expressed in gastric tumors and to analyze the association of their expression level with tumor clinicopathologic features. Methods: In the present research, we used bioinformatic-driven search to identify miRNA that are down-regulated in gastric tumors and to find their potential targets. Then, the expression levels of some of the target mRNAs were investigated using reverse transcription polymerase chain reaction (RT-PCR) analysis. Results: As a result of the bioinformatics analysis, fifteen genes were found to be potentially differentially expressed between the tumors and normal gastric tissue. Five of them were chosen for the further analysis (WNT4, FGF12, EFEMP1, CTGF, and HSPG2) due to their important role in cell proliferation and differentiation. Expression levels of these genes were evaluated in our collection of frozen tissue samples of gastric tumor and paired normal stomach epithelia. Increased FGF12 expression was observed in diffuse type of gastric cancer while WNT4 mRNA was found to be down-regulated in intestinal type of gastric cancer. Besides, CTGF gene overexpression was revealed in diffuse type of stomach cancer in comparison with that in intestinal type. Up-regulation of CTGF was also associated with lymph node metastasis. Conclusions: The findings show its expedient to perform further investigations in order to clarify diagnostic and prognostic value of CTGF, FGF12, and WNT4’s in stomach cancer as well as the role of these genes in carcinogenesis.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Search for potential gastric cancer markers using miRNA databases and gene expression analysis
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Search for potential gastric cancer markers using miRNA databases and gene expression analysis
spellingShingle Search for potential gastric cancer markers using miRNA databases and gene expression analysis
Volkomorov, V.
Grigoryeva, E.
Krasnov, G.
Litviakov, N.
Tsyganov, M.
Karbyshev, M.
Zavyalova, M.
Afanasyev, S.
Cherdyntseva, N.
Lisitsyn, N.
Beresten, S.
Original contributions
title_short Search for potential gastric cancer markers using miRNA databases and gene expression analysis
title_full Search for potential gastric cancer markers using miRNA databases and gene expression analysis
title_fullStr Search for potential gastric cancer markers using miRNA databases and gene expression analysis
title_full_unstemmed Search for potential gastric cancer markers using miRNA databases and gene expression analysis
title_sort search for potential gastric cancer markers using mirna databases and gene expression analysis
author Volkomorov, V.
Grigoryeva, E.
Krasnov, G.
Litviakov, N.
Tsyganov, M.
Karbyshev, M.
Zavyalova, M.
Afanasyev, S.
Cherdyntseva, N.
Lisitsyn, N.
Beresten, S.
author_facet Volkomorov, V.
Grigoryeva, E.
Krasnov, G.
Litviakov, N.
Tsyganov, M.
Karbyshev, M.
Zavyalova, M.
Afanasyev, S.
Cherdyntseva, N.
Lisitsyn, N.
Beresten, S.
topic Original contributions
topic_facet Original contributions
publishDate 2013
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: The aim of this study was to identify genes that are differentially expressed in gastric tumors and to analyze the association of their expression level with tumor clinicopathologic features. Methods: In the present research, we used bioinformatic-driven search to identify miRNA that are down-regulated in gastric tumors and to find their potential targets. Then, the expression levels of some of the target mRNAs were investigated using reverse transcription polymerase chain reaction (RT-PCR) analysis. Results: As a result of the bioinformatics analysis, fifteen genes were found to be potentially differentially expressed between the tumors and normal gastric tissue. Five of them were chosen for the further analysis (WNT4, FGF12, EFEMP1, CTGF, and HSPG2) due to their important role in cell proliferation and differentiation. Expression levels of these genes were evaluated in our collection of frozen tissue samples of gastric tumor and paired normal stomach epithelia. Increased FGF12 expression was observed in diffuse type of gastric cancer while WNT4 mRNA was found to be down-regulated in intestinal type of gastric cancer. Besides, CTGF gene overexpression was revealed in diffuse type of stomach cancer in comparison with that in intestinal type. Up-regulation of CTGF was also associated with lymph node metastasis. Conclusions: The findings show its expedient to perform further investigations in order to clarify diagnostic and prognostic value of CTGF, FGF12, and WNT4’s in stomach cancer as well as the role of these genes in carcinogenesis.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/139132
citation_txt Search for potential gastric cancer markers using miRNA databases and gene expression analysis / V. Volkomorov, E. Grigoryeva, G. Krasnov, N. Litviakov, M. Tsyganov, M. Karbyshev, M. Zavyalova, S. Afanasyev, N. Cherdyntseva, N. Lisitsyn, S. Beresten // Experimental Oncology. — 2013. — Т. 35, № 1. — С. 2-7. — Бібліогр.: 37 назв. — англ.
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first_indexed 2025-12-07T16:53:13Z
last_indexed 2025-12-07T16:53:13Z
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