Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines

Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines

The aim of the study was to analyze an effect of cytostatic agents of different mechanism of action on expression levels of human beta-defensins-1-4 (hBD-1-4) in cultured human cancer cell lines. Materials and Methods: Expression levels of hBD-1-4 mRNA were assessed using qPCR in human epidermoid ca...

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Бібліографічні деталі
Опубліковано в: :Experimental Oncology
Дата:2018
Автори: Zubenko, O.S., Semeniuk, D.O., Starenka, I.O., Pogribnyy, P.V.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2018
Теми:
Short communications
Онлайн доступ:https://nasplib.isofts.kiev.ua/handle/123456789/139241
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines / O.S. Zubenko, D.O. Semeniuk, I.O. Starenka, P.V. Pogribnyy // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 79-81. — Бібліогр.: 6 назв. — англ.

Репозитарії

Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-139241
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spelling Zubenko, O.S.
Semeniuk, D.O.
Starenka, I.O.
Pogribnyy, P.V.
2018-06-19T20:58:17Z
2018-06-19T20:58:17Z
2018
Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines / O.S. Zubenko, D.O. Semeniuk, I.O. Starenka, P.V. Pogribnyy // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 79-81. — Бібліогр.: 6 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/139241
The aim of the study was to analyze an effect of cytostatic agents of different mechanism of action on expression levels of human beta-defensins-1-4 (hBD-1-4) in cultured human cancer cell lines. Materials and Methods: Expression levels of hBD-1-4 mRNA were assessed using qPCR in human epidermoid carcinoma A431 cells and human breast adenocarcinoma MCF7 cells treated with cisplatin, methotrexate, doxorubicin or vincristine at the IC20 concentrations. Results: The cytostatic agents with different mechanisms of action affected differently expression of hBDs, dependent on the cell line. Mostly, cytostatic agents suppressed significantly expression of hBDs. In contrast, vincristine caused significant up-regulation of hBD-1 (12 fold, p < 0.05) and hBD-4 (2 fold, p < 0.05) in MCF7, and doxorubicin significantly enhanced expression of hBD-3 (2 fold, p < 0.05) and hBD-4 (> 10 fold, p < 0.05) in A431 cells. Conclusion: The results of this pilot study show that expression levels of hBD-1-4 may be altered upon treatment with cytostatic agents depending on nature of cells.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Short communications
Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
spellingShingle Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
Zubenko, O.S.
Semeniuk, D.O.
Starenka, I.O.
Pogribnyy, P.V.
Short communications
title_short Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
title_full Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
title_fullStr Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
title_full_unstemmed Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
title_sort effect of cytostatic agents on expression levels of human beta-defensins-1-4 in a431 and mcf-7 cell lines
author Zubenko, O.S.
Semeniuk, D.O.
Starenka, I.O.
Pogribnyy, P.V.
author_facet Zubenko, O.S.
Semeniuk, D.O.
Starenka, I.O.
Pogribnyy, P.V.
topic Short communications
topic_facet Short communications
publishDate 2018
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description The aim of the study was to analyze an effect of cytostatic agents of different mechanism of action on expression levels of human beta-defensins-1-4 (hBD-1-4) in cultured human cancer cell lines. Materials and Methods: Expression levels of hBD-1-4 mRNA were assessed using qPCR in human epidermoid carcinoma A431 cells and human breast adenocarcinoma MCF7 cells treated with cisplatin, methotrexate, doxorubicin or vincristine at the IC20 concentrations. Results: The cytostatic agents with different mechanisms of action affected differently expression of hBDs, dependent on the cell line. Mostly, cytostatic agents suppressed significantly expression of hBDs. In contrast, vincristine caused significant up-regulation of hBD-1 (12 fold, p < 0.05) and hBD-4 (2 fold, p < 0.05) in MCF7, and doxorubicin significantly enhanced expression of hBD-3 (2 fold, p < 0.05) and hBD-4 (> 10 fold, p < 0.05) in A431 cells. Conclusion: The results of this pilot study show that expression levels of hBD-1-4 may be altered upon treatment with cytostatic agents depending on nature of cells.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/139241
citation_txt Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines / O.S. Zubenko, D.O. Semeniuk, I.O. Starenka, P.V. Pogribnyy // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 79-81. — Бібліогр.: 6 назв. — англ.
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