Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients
Background: The development of hormone-dependent cancers, including endometrial carcinomas, in great part may be mediated by the genotoxic effects of estrogen metabolites, among which 4-hydroxyestradiol (4OHE2) is characterized by the most prominent DNA-damaging properties. It is assumed that the in...
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| Опубліковано в: : | Experimental Oncology |
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| Дата: | 2018 |
| Автори: | , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2018
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| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/139243 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients / L.G. Buchynska, O.V. Brieieva // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 68–72. — Бібліогр.: 28 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862643820287492096 |
|---|---|
| author | Buchynska, L.G. Brieieva, O.V. |
| author_facet | Buchynska, L.G. Brieieva, O.V. |
| citation_txt | Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients / L.G. Buchynska, O.V. Brieieva // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 68–72. — Бібліогр.: 28 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Background: The development of hormone-dependent cancers, including endometrial carcinomas, in great part may be mediated by the genotoxic effects of estrogen metabolites, among which 4-hydroxyestradiol (4OHE2) is characterized by the most prominent DNA-damaging properties. It is assumed that the individual sensitivity to the 4OHE2 may determine the predisposition to endometrial cancer (EС). Aim: To analyze the sensitivity of peripheral blood lymphocytes (PBLs) of EC patients to the 4OHE2 and to evaluate the repair efficiency of 4OHE2-induced DNA damage. Materials and Methods: The study was performed on the PBLs of 53 EC patients and 20 healthy women. The level of DNA damage was measured using the comet assay and was expressed as % tail DNA. The DNA repair efficiency (%) was evaluated by determining the ratio between the amount of repaired DNA damage and the level of 4OHE2-induced damage that appeared after incubation of PBLs with 4OHE2. Results: In PBLs of EC patients, a higher level of 4OHE2-induced DNA damage (32.0 ± 2.2% tail DNA) and lower DNA repair efficiency (34.0 ± 4.5%) was observed compared to PBLs of healthy women (22.3 ± 2.3% tail DNA and 48.8 ± 4.5%, respectively). PBLs of EC patients with deep tumor invasion of myometrium were characterized by more prominent decrease of DNA repair than those with less invasive tumor (< ½ of myometrium) (20.9 ± 7.8 and 43.7 ± 6.7%, respectively). Furthermore, lower DNA repair efficiency was detected in the PBLs of EC patients with a family history of cancer compared to this parameter in patients with sporadic tumors (20.9±7.8 and 47.1 ± 5.5%, respectively). Conclusion: The PBLs of EC patients are characterized by increased sensitivity to the genotoxic effect of 4OHE2 and reduced repair efficiency regarding 4OHE2-induced DNA damage. A lower level of DNA repair is observed in EC patients with deep tumor myometrial invasion and a family history of cancer.
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| first_indexed | 2025-12-01T08:13:04Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-139243 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-01T08:13:04Z |
| publishDate | 2018 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Buchynska, L.G. Brieieva, O.V. 2018-06-19T21:01:24Z 2018-06-19T21:01:24Z 2018 Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients / L.G. Buchynska, O.V. Brieieva // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 68–72. — Бібліогр.: 28 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/139243 Background: The development of hormone-dependent cancers, including endometrial carcinomas, in great part may be mediated by the genotoxic effects of estrogen metabolites, among which 4-hydroxyestradiol (4OHE2) is characterized by the most prominent DNA-damaging properties. It is assumed that the individual sensitivity to the 4OHE2 may determine the predisposition to endometrial cancer (EС). Aim: To analyze the sensitivity of peripheral blood lymphocytes (PBLs) of EC patients to the 4OHE2 and to evaluate the repair efficiency of 4OHE2-induced DNA damage. Materials and Methods: The study was performed on the PBLs of 53 EC patients and 20 healthy women. The level of DNA damage was measured using the comet assay and was expressed as % tail DNA. The DNA repair efficiency (%) was evaluated by determining the ratio between the amount of repaired DNA damage and the level of 4OHE2-induced damage that appeared after incubation of PBLs with 4OHE2. Results: In PBLs of EC patients, a higher level of 4OHE2-induced DNA damage (32.0 ± 2.2% tail DNA) and lower DNA repair efficiency (34.0 ± 4.5%) was observed compared to PBLs of healthy women (22.3 ± 2.3% tail DNA and 48.8 ± 4.5%, respectively). PBLs of EC patients with deep tumor invasion of myometrium were characterized by more prominent decrease of DNA repair than those with less invasive tumor (< ½ of myometrium) (20.9 ± 7.8 and 43.7 ± 6.7%, respectively). Furthermore, lower DNA repair efficiency was detected in the PBLs of EC patients with a family history of cancer compared to this parameter in patients with sporadic tumors (20.9±7.8 and 47.1 ± 5.5%, respectively). Conclusion: The PBLs of EC patients are characterized by increased sensitivity to the genotoxic effect of 4OHE2 and reduced repair efficiency regarding 4OHE2-induced DNA damage. A lower level of DNA repair is observed in EC patients with deep tumor myometrial invasion and a family history of cancer. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients Article published earlier |
| spellingShingle | Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients Buchynska, L.G. Brieieva, O.V. Original contributions |
| title | Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_full | Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_fullStr | Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_full_unstemmed | Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_short | Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_sort | sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/139243 |
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