Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients
Aim: To determine whether the structural and functional diversities of naturally occurring antibodies to the Thomsen — Friedenreich (TF) antigen may be of diagnostic and prognostic value in colon cancer. Materials and Methods: Serum samples were taken from patients with colon cancer (n = 94) and hea...
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| Published in: | Experimental Oncology |
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| Date: | 2018 |
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2018
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/139250 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients / O. Kurtenkov, M. Bubina, K. Klaamas // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 48-58. — Бібліогр.: 56 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862720250788708352 |
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| author | Kurtenkov, O. Bubina, M. Klaamas, K. |
| author_facet | Kurtenkov, O. Bubina, M. Klaamas, K. |
| citation_txt | Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients / O. Kurtenkov, M. Bubina, K. Klaamas // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 48-58. — Бібліогр.: 56 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To determine whether the structural and functional diversities of naturally occurring antibodies to the Thomsen — Friedenreich (TF) antigen may be of diagnostic and prognostic value in colon cancer. Materials and Methods: Serum samples were taken from patients with colon cancer (n = 94) and healthy controls (n = 64). The level of TF-specific antibody isotypes and their sialylation were determined using ELISA and lectin-ELISA with synthetic TF-polyacrylamide conjugate as an antigen and a sialic acid-specific Sambucus nigra agglutinin (SNA). The avidity was determined using ammonium thiocyanate as a chaotrope. The accuracy of diagnostics was evaluated using the receiver operator characteristic curve analysis and the survival analysis employing the Kaplan — Meier method. Results: Compared to healthy controls, patients with colon cancer exhibited a lower level of anti-TF IgG antibodies, significantly lower ratios of TF-specific IgG/IgM and IgG/IgA, an increased SNA reactivity of anti-TF antibodies, mostly on account of IgG, and a lower avidity of TF-specific antibodies, especially their SNA-reactive subset. An increased SNA reactivity of anti-TF IgG was observed already at the early stages of cancer (p = 0.0004). The decrease of the ratio of IgG/IgM and IgG/IgA showed a good accuracy of diagnostics with about 60% sensitivity at 90% specificity. A similar potential was found for the SNA binding/IgG level index. The high level of TF-specific IgA antibodies was associated with a lower survival rate (hazard ratio = 0.34). Conclusion: This is the first report ever on the colon cancer-related signatures of anti-TF antibody diversity which show diagnostic potential, including in early cancer, and prognostic value. The hypersialylation of TF-specific antibodies appeared to be a common phenomenon in cancer. The signatures may be used as non-invasive antibody-based markers for colon cancer.
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| first_indexed | 2025-12-07T18:24:24Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-139250 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T18:24:24Z |
| publishDate | 2018 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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| spelling | Kurtenkov, O. Bubina, M. Klaamas, K. 2018-06-19T21:07:46Z 2018-06-19T21:07:46Z 2018 Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients / O. Kurtenkov, M. Bubina, K. Klaamas // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 48-58. — Бібліогр.: 56 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/139250 Aim: To determine whether the structural and functional diversities of naturally occurring antibodies to the Thomsen — Friedenreich (TF) antigen may be of diagnostic and prognostic value in colon cancer. Materials and Methods: Serum samples were taken from patients with colon cancer (n = 94) and healthy controls (n = 64). The level of TF-specific antibody isotypes and their sialylation were determined using ELISA and lectin-ELISA with synthetic TF-polyacrylamide conjugate as an antigen and a sialic acid-specific Sambucus nigra agglutinin (SNA). The avidity was determined using ammonium thiocyanate as a chaotrope. The accuracy of diagnostics was evaluated using the receiver operator characteristic curve analysis and the survival analysis employing the Kaplan — Meier method. Results: Compared to healthy controls, patients with colon cancer exhibited a lower level of anti-TF IgG antibodies, significantly lower ratios of TF-specific IgG/IgM and IgG/IgA, an increased SNA reactivity of anti-TF antibodies, mostly on account of IgG, and a lower avidity of TF-specific antibodies, especially their SNA-reactive subset. An increased SNA reactivity of anti-TF IgG was observed already at the early stages of cancer (p = 0.0004). The decrease of the ratio of IgG/IgM and IgG/IgA showed a good accuracy of diagnostics with about 60% sensitivity at 90% specificity. A similar potential was found for the SNA binding/IgG level index. The high level of TF-specific IgA antibodies was associated with a lower survival rate (hazard ratio = 0.34). Conclusion: This is the first report ever on the colon cancer-related signatures of anti-TF antibody diversity which show diagnostic potential, including in early cancer, and prognostic value. The hypersialylation of TF-specific antibodies appeared to be a common phenomenon in cancer. The signatures may be used as non-invasive antibody-based markers for colon cancer. This work was supported by the Estonian Research Council Grant PUT371. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients Article published earlier |
| spellingShingle | Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients Kurtenkov, O. Bubina, M. Klaamas, K. Original contributions |
| title | Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients |
| title_full | Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients |
| title_fullStr | Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients |
| title_full_unstemmed | Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients |
| title_short | Signatures of anti-Thomsen — friedenreich antigen antibody diversity in colon cancer patients |
| title_sort | signatures of anti-thomsen — friedenreich antigen antibody diversity in colon cancer patients |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/139250 |
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