Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy
Aim: Methotrexate (MTX) is an antifolate agent that acts inhibiting purine and pyrimidine synthesis. The objective of the study was to evaluate the viability of Hep-2 human laryngeal cancer cells to the treatment with MTX chemotherapy in vitro. Methods: Cultured Hep-2 cells were treated with 0.25, 2...
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| Published in: | Experimental Oncology |
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| Date: | 2012 |
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| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2012
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/139856 |
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| Cite this: | Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy / A.L.S. Galbiatti, H.C. Caldas, J.A. Padovani Junior, E.C. Pavarino, E.M. Goloni-Bertollo // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 367-369. — Бібліогр.: 22 назв. — англ. |
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Galbiatti, A.L.S. Caldas, H.C. Padovani Junior, J.A. Pavarino, E.C. Goloni-Bertollo, E.M. 2018-06-21T12:37:44Z 2018-06-21T12:37:44Z 2012 Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy / A.L.S. Galbiatti, H.C. Caldas, J.A. Padovani Junior, E.C. Pavarino, E.M. Goloni-Bertollo // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 367-369. — Бібліогр.: 22 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/139856 Aim: Methotrexate (MTX) is an antifolate agent that acts inhibiting purine and pyrimidine synthesis. The objective of the study was to evaluate the viability of Hep-2 human laryngeal cancer cells to the treatment with MTX chemotherapy in vitro. Methods: Cultured Hep-2 cells were treated with 0.25, 25.0 and 75 μM MTX for 24 h, and their viability was evaluated with Bcl-2-FITC antibody in flow cytometry. Results: The numbers of viable Hep-2 cells after 24 h treatment with 0.25, 25.0 and 75.0 uM MTX were 85.43%, 22.46% and 8.42%, respectively (p < 0.05). Therefore, MTX possesses a dose-dependent effect on viability of Hep-2 cells in vitro. Conclusion: The highest MTX concentration is associated with highest tumor cell sensitivity of human laryngeal cancer cells of Hep-2 line. Fundação de Amparo а Pesquisa do Estado de Sгo Paulo (FAPESP) for their financial support (Nє 2010/12930-4, 2010/12932-7); Prof. Dr. Moacir F. Godoy for their help in statistical analysis; Profa. Dra Eloiza Helena Tajara for providing of the cell line; Oncology Department, Hospital de Base, São José do Rio Preto for providing of the Methotrexate Chemotherapeutic; CNPQ (National Counsel of Technological and Scientific Development) and FAMERP/FUNFARME. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Short communications Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy Article published earlier |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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| title |
Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy |
| spellingShingle |
Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy Galbiatti, A.L.S. Caldas, H.C. Padovani Junior, J.A. Pavarino, E.C. Goloni-Bertollo, E.M. Short communications |
| title_short |
Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy |
| title_full |
Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy |
| title_fullStr |
Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy |
| title_full_unstemmed |
Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy |
| title_sort |
sensitivity of human laryngeal squamous cell carcinoma hep-2 to metrotexate chemoterapy |
| author |
Galbiatti, A.L.S. Caldas, H.C. Padovani Junior, J.A. Pavarino, E.C. Goloni-Bertollo, E.M. |
| author_facet |
Galbiatti, A.L.S. Caldas, H.C. Padovani Junior, J.A. Pavarino, E.C. Goloni-Bertollo, E.M. |
| topic |
Short communications |
| topic_facet |
Short communications |
| publishDate |
2012 |
| language |
English |
| container_title |
Experimental Oncology |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| format |
Article |
| description |
Aim: Methotrexate (MTX) is an antifolate agent that acts inhibiting purine and pyrimidine synthesis. The objective of the study was to evaluate the viability of Hep-2 human laryngeal cancer cells to the treatment with MTX chemotherapy in vitro. Methods: Cultured Hep-2 cells were treated with 0.25, 25.0 and 75 μM MTX for 24 h, and their viability was evaluated with Bcl-2-FITC antibody in flow cytometry. Results: The numbers of viable Hep-2 cells after 24 h treatment with 0.25, 25.0 and 75.0 uM MTX were 85.43%, 22.46% and 8.42%, respectively (p < 0.05). Therefore, MTX possesses a dose-dependent effect on viability of Hep-2 cells in vitro. Conclusion: The highest MTX concentration is associated with highest tumor cell sensitivity of human laryngeal cancer cells of Hep-2 line.
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| issn |
1812-9269 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/139856 |
| citation_txt |
Sensitivity of human laryngeal squamous cell carcinoma Hep-2 to metrotexate chemoterapy / A.L.S. Galbiatti, H.C. Caldas, J.A. Padovani Junior, E.C. Pavarino, E.M. Goloni-Bertollo // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 367-369. — Бібліогр.: 22 назв. — англ. |
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2025-12-07T19:56:35Z |
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