Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon

Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon

In research of the last decade, rhythmic (circadian) variations of vascular endothelial growth factor (VEGF) production by tumors were discovered. The present paper authors have earlier synthesized and characterized a new derivative photosensitizer — an immunoconjugate of hematoporphyrin with antiVE...

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Veröffentlicht in:Experimental Oncology
Datum:2012
Hauptverfasser: Gamaleia, N.F., Lisnyak, I.A., Shishko, E.D., Mamchur, A.A., Prokopenko, I.V., Kholin, V.V.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2012
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Short communications
Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/139860
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Zitieren:Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon / N.F. Gamaleia, I.A. Lisnyak, E.D. Shishko, A.A. Mamchur, I.V. Prokopenko, V.V. Kholin // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 364-366. — Бібліогр.: 11 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-139860
record_format dspace
spelling Gamaleia, N.F.
Lisnyak, I.A.
Shishko, E.D.
Mamchur, A.A.
Prokopenko, I.V.
Kholin, V.V.
2018-06-21T12:41:35Z
2018-06-21T12:41:35Z
2012
Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon / N.F. Gamaleia, I.A. Lisnyak, E.D. Shishko, A.A. Mamchur, I.V. Prokopenko, V.V. Kholin // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 364-366. — Бібліогр.: 11 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/139860
In research of the last decade, rhythmic (circadian) variations of vascular endothelial growth factor (VEGF) production by tumors were discovered. The present paper authors have earlier synthesized and characterized a new derivative photosensitizer — an immunoconjugate of hematoporphyrin with antiVEGF antibodies. Aim: To elaborate and to test a novel modification of the photodynamic therapy of tumors (PDT) method, founding upon a timed introduction of the immunoconjugated photosensitizer to tumor-bearing animals, so that this coincides with a maximum content of VEGF in tumor tissues. Methods: Circadian variations of VEGF contents in murine transplanted tumors, Lewis lung carcinoma and sarcoma 180, were determined by ELISA method. Immunoconjugated photosensitizer concentrations in tumors were estimated by spectrofluorometry. Photoirradiation of the tumors was carried out with a red light (wavelength of 635 nm) from a semiconductor laser. Light doses were chosen, calculating on a partial inhibition of tumor growth, in order that a dependence of PDT efficiency on a daily time-moment (circadian rhythm phase) of the treatment could be observed distinctly. Results: Circadian variations of the VEGF levels in Lewis lung carcinoma and sarcoma 180 were demonstrated with the maximum at 14:00 h and the minimum at 02:00 h. Intra-abdominal introduction into tumor-bearing mice of the immunoconjugated photosensitizer resulted in a greater accumulation of the immunoconjugate in tumors at 14:00 h than at 02:00 h. Laser irradiation of carcinomas and sarcomas at 14:00 h or 02:00 h after introduction of the immunoconjugated photosensitizer to mice the day before at the same time points, induced a significantly enhanced inhibition of tumor growth in animals treated at day-time versus those treated at night-time. Conclusion: The obtained results justify further attempts to transfer principles of tumor chronochemotherapy onto photodynamic therapy.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Short communications
Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon
spellingShingle Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon
Gamaleia, N.F.
Lisnyak, I.A.
Shishko, E.D.
Mamchur, A.A.
Prokopenko, I.V.
Kholin, V.V.
Short communications
title_short Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon
title_full Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon
title_fullStr Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon
title_full_unstemmed Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon
title_sort chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluation
author Gamaleia, N.F.
Lisnyak, I.A.
Shishko, E.D.
Mamchur, A.A.
Prokopenko, I.V.
Kholin, V.V.
author_facet Gamaleia, N.F.
Lisnyak, I.A.
Shishko, E.D.
Mamchur, A.A.
Prokopenko, I.V.
Kholin, V.V.
topic Short communications
topic_facet Short communications
publishDate 2012
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description In research of the last decade, rhythmic (circadian) variations of vascular endothelial growth factor (VEGF) production by tumors were discovered. The present paper authors have earlier synthesized and characterized a new derivative photosensitizer — an immunoconjugate of hematoporphyrin with antiVEGF antibodies. Aim: To elaborate and to test a novel modification of the photodynamic therapy of tumors (PDT) method, founding upon a timed introduction of the immunoconjugated photosensitizer to tumor-bearing animals, so that this coincides with a maximum content of VEGF in tumor tissues. Methods: Circadian variations of VEGF contents in murine transplanted tumors, Lewis lung carcinoma and sarcoma 180, were determined by ELISA method. Immunoconjugated photosensitizer concentrations in tumors were estimated by spectrofluorometry. Photoirradiation of the tumors was carried out with a red light (wavelength of 635 nm) from a semiconductor laser. Light doses were chosen, calculating on a partial inhibition of tumor growth, in order that a dependence of PDT efficiency on a daily time-moment (circadian rhythm phase) of the treatment could be observed distinctly. Results: Circadian variations of the VEGF levels in Lewis lung carcinoma and sarcoma 180 were demonstrated with the maximum at 14:00 h and the minimum at 02:00 h. Intra-abdominal introduction into tumor-bearing mice of the immunoconjugated photosensitizer resulted in a greater accumulation of the immunoconjugate in tumors at 14:00 h than at 02:00 h. Laser irradiation of carcinomas and sarcomas at 14:00 h or 02:00 h after introduction of the immunoconjugated photosensitizer to mice the day before at the same time points, induced a significantly enhanced inhibition of tumor growth in animals treated at day-time versus those treated at night-time. Conclusion: The obtained results justify further attempts to transfer principles of tumor chronochemotherapy onto photodynamic therapy.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/139860
citation_txt Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon / N.F. Gamaleia, I.A. Lisnyak, E.D. Shishko, A.A. Mamchur, I.V. Prokopenko, V.V. Kholin // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 364-366. — Бібліогр.: 11 назв. — англ.
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first_indexed 2025-12-07T17:05:06Z
last_indexed 2025-12-07T17:05:06Z
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