Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon
In research of the last decade, rhythmic (circadian) variations of vascular endothelial growth factor (VEGF) production by tumors were discovered. The present paper authors have earlier synthesized and characterized a new derivative photosensitizer — an immunoconjugate of hematoporphyrin with antiVE...
Збережено в:
| Опубліковано в: : | Experimental Oncology |
|---|---|
| Дата: | 2012 |
| Автори: | , , , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2012
|
| Теми: | |
| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/139860 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon / N.F. Gamaleia, I.A. Lisnyak, E.D. Shishko, A.A. Mamchur, I.V. Prokopenko, V.V. Kholin // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 364-366. — Бібліогр.: 11 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862707400172109824 |
|---|---|
| author | Gamaleia, N.F. Lisnyak, I.A. Shishko, E.D. Mamchur, A.A. Prokopenko, I.V. Kholin, V.V. |
| author_facet | Gamaleia, N.F. Lisnyak, I.A. Shishko, E.D. Mamchur, A.A. Prokopenko, I.V. Kholin, V.V. |
| citation_txt | Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon / N.F. Gamaleia, I.A. Lisnyak, E.D. Shishko, A.A. Mamchur, I.V. Prokopenko, V.V. Kholin // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 364-366. — Бібліогр.: 11 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | In research of the last decade, rhythmic (circadian) variations of vascular endothelial growth factor (VEGF) production by tumors were discovered. The present paper authors have earlier synthesized and characterized a new derivative photosensitizer — an immunoconjugate of hematoporphyrin with antiVEGF antibodies. Aim: To elaborate and to test a novel modification of the photodynamic therapy of tumors (PDT) method, founding upon a timed introduction of the immunoconjugated photosensitizer to tumor-bearing animals, so that this coincides with a maximum content of VEGF in tumor tissues. Methods: Circadian variations of VEGF contents in murine transplanted tumors, Lewis lung carcinoma and sarcoma 180, were determined by ELISA method. Immunoconjugated photosensitizer concentrations in tumors were estimated by spectrofluorometry. Photoirradiation of the tumors was carried out with a red light (wavelength of 635 nm) from a semiconductor laser. Light doses were chosen, calculating on a partial inhibition of tumor growth, in order that a dependence of PDT efficiency on a daily time-moment (circadian rhythm phase) of the treatment could be observed distinctly. Results: Circadian variations of the VEGF levels in Lewis lung carcinoma and sarcoma 180 were demonstrated with the maximum at 14:00 h and the minimum at 02:00 h. Intra-abdominal introduction into tumor-bearing mice of the immunoconjugated photosensitizer resulted in a greater accumulation of the immunoconjugate in tumors at 14:00 h than at 02:00 h. Laser irradiation of carcinomas and sarcomas at 14:00 h or 02:00 h after introduction of the immunoconjugated photosensitizer to mice the day before at the same time points, induced a significantly enhanced inhibition of tumor growth in animals treated at day-time versus those treated at night-time. Conclusion: The obtained results justify further attempts to transfer principles of tumor chronochemotherapy onto photodynamic therapy.
|
| first_indexed | 2025-12-07T17:05:06Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-139860 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T17:05:06Z |
| publishDate | 2012 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Gamaleia, N.F. Lisnyak, I.A. Shishko, E.D. Mamchur, A.A. Prokopenko, I.V. Kholin, V.V. 2018-06-21T12:41:35Z 2018-06-21T12:41:35Z 2012 Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon / N.F. Gamaleia, I.A. Lisnyak, E.D. Shishko, A.A. Mamchur, I.V. Prokopenko, V.V. Kholin // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 364-366. — Бібліогр.: 11 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/139860 In research of the last decade, rhythmic (circadian) variations of vascular endothelial growth factor (VEGF) production by tumors were discovered. The present paper authors have earlier synthesized and characterized a new derivative photosensitizer — an immunoconjugate of hematoporphyrin with antiVEGF antibodies. Aim: To elaborate and to test a novel modification of the photodynamic therapy of tumors (PDT) method, founding upon a timed introduction of the immunoconjugated photosensitizer to tumor-bearing animals, so that this coincides with a maximum content of VEGF in tumor tissues. Methods: Circadian variations of VEGF contents in murine transplanted tumors, Lewis lung carcinoma and sarcoma 180, were determined by ELISA method. Immunoconjugated photosensitizer concentrations in tumors were estimated by spectrofluorometry. Photoirradiation of the tumors was carried out with a red light (wavelength of 635 nm) from a semiconductor laser. Light doses were chosen, calculating on a partial inhibition of tumor growth, in order that a dependence of PDT efficiency on a daily time-moment (circadian rhythm phase) of the treatment could be observed distinctly. Results: Circadian variations of the VEGF levels in Lewis lung carcinoma and sarcoma 180 were demonstrated with the maximum at 14:00 h and the minimum at 02:00 h. Intra-abdominal introduction into tumor-bearing mice of the immunoconjugated photosensitizer resulted in a greater accumulation of the immunoconjugate in tumors at 14:00 h than at 02:00 h. Laser irradiation of carcinomas and sarcomas at 14:00 h or 02:00 h after introduction of the immunoconjugated photosensitizer to mice the day before at the same time points, induced a significantly enhanced inhibition of tumor growth in animals treated at day-time versus those treated at night-time. Conclusion: The obtained results justify further attempts to transfer principles of tumor chronochemotherapy onto photodynamic therapy. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Short communications Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon Article published earlier |
| spellingShingle | Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon Gamaleia, N.F. Lisnyak, I.A. Shishko, E.D. Mamchur, A.A. Prokopenko, I.V. Kholin, V.V. Short communications |
| title | Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon |
| title_full | Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon |
| title_fullStr | Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon |
| title_full_unstemmed | Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon |
| title_short | Chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluatIon |
| title_sort | chronobiological approaches to antiangiogenic photodynamic therapy of tumors: the first experimental evaluation |
| topic | Short communications |
| topic_facet | Short communications |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/139860 |
| work_keys_str_mv | AT gamaleianf chronobiologicalapproachestoantiangiogenicphotodynamictherapyoftumorsthefirstexperimentalevaluation AT lisnyakia chronobiologicalapproachestoantiangiogenicphotodynamictherapyoftumorsthefirstexperimentalevaluation AT shishkoed chronobiologicalapproachestoantiangiogenicphotodynamictherapyoftumorsthefirstexperimentalevaluation AT mamchuraa chronobiologicalapproachestoantiangiogenicphotodynamictherapyoftumorsthefirstexperimentalevaluation AT prokopenkoiv chronobiologicalapproachestoantiangiogenicphotodynamictherapyoftumorsthefirstexperimentalevaluation AT kholinvv chronobiologicalapproachestoantiangiogenicphotodynamictherapyoftumorsthefirstexperimentalevaluation |