Effects of combined sonodynamic and photodynamic therapies with photolon on a glioma C6 tumor model

• Published in: Experimental Oncology
• Date: 2012
• Main Authors: Tserkovsky, D.A., Alexandrova, E.N., Chalau, V.N., Istomin, Yu.P.
• Format: Article
• Language: English
• Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2012
• Subjects: Original contributions
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/139874
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Effects of combined sonodynamic and photodynamic therapies with photolon on a glioma C6 tumor model / D.A. Tserkovsky, E.N. Alexandrova, V.N. Chalau, Yu.Р. Istomin // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 332-335. — Бібліогр.: 16 назв. — англ.

The aim of this study was to investigate the low-power density sonication, sonodynamic therapy (SDT) with Photolon and combination of SDT and photodynamic therapy (PDT) with Photolon for the ablation of glioma C6 tumor model in rats. Methods: The study was performed on 50 rats bearing glioma C6. The tumors were sonicated with/without prior intravenous injection of photosensitizer (PS) Photolon (2.5 mg/kg b.w). Sonication was performed with 0.4; 0.7 and 1.0 W/cm² power density at 1 MHz frequency for 10 min, 2.0 h after Photolon administration using BTL-5710 Sono (BTL Industries Limited, Great Britain). PDT was carried out 2.5 h after Photolon administration using diode laser with 661 nm wavelength (IMAF-AXICON, Minsk, Republic of Belarus) at doses of 50 and 100 J/cm² with 0,17 W/cm² fluence rate. Assessment of tumor response was performed by vital staining with Evans blue and pathologic examination. Results: The maximal tumor necrosis area that underwent sonication (1 MHz; 0.7 W/cm²; 10 min.) followed by PDT at a dose of 100 J/cm² was 100%. Conclusion: This is the first report to demonstrate the benefits of sono-photodynamic therapy (SPDT) consisting of low-power density ultrasound and PDT for the treatment of malignant glioma models.