Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer

Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer

Aim:To assess the frequency and spectrum of chromosome aberrations and micronuclei in peripheral blood lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer during the course of neutron therapy. Materials and Methods: Samples of peripheral blood were obtained from 9...

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Veröffentlicht in:Experimental Oncology
Datum:2012
Hauptverfasser: Melnikov, A.A., Vasilyev, S.A., Musabaeva, L.I., Velikaya, V.V., Gribova, O.V., Startseva, Zh.A., Urazova, L.N., Lebedev, I.N., Choynzonov, E.L.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2012
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/139878
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Zitieren:Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer / A.A. Melnikov, S.A. Vasilyev, L.I. Musabaeva, V.V. Velikaya, O.V. Gribova, Zh.A. Startseva, L.N. Urazova, I.N. Lebedev, E.L. Choynzonov // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 354-357. — Бібліогр.: 19 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-139878
record_format dspace
spelling Melnikov, A.A.
Vasilyev, S.A.
Musabaeva, L.I.
Velikaya, V.V.
Gribova, O.V.
Startseva, Zh.A.
Urazova, L.N.
Lebedev, I.N.
Choynzonov, E.L.
2018-06-21T12:59:35Z
2018-06-21T12:59:35Z
2012
Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer / A.A. Melnikov, S.A. Vasilyev, L.I. Musabaeva, V.V. Velikaya, O.V. Gribova, Zh.A. Startseva, L.N. Urazova, I.N. Lebedev, E.L. Choynzonov // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 354-357. — Бібліогр.: 19 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/139878
Aim:To assess the frequency and spectrum of chromosome aberrations and micronuclei in peripheral blood lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer during the course of neutron therapy. Materials and Methods: Samples of peripheral blood were obtained from 9 patients with parotid salivary gland tumors (T3N0–3M0) and 8 patients with relapse of breast cancer before, after first fraction and at the end of neutron therapy. The treatment course specified 5.5–8.4 Gy (equivalent to 23–44 Gy of photon irradiation) with 1.3–2.2 Gy per fraction for patients with parotid salivary gland tumors and 4,8–8.0 Gy (equivalent to 30–40 Gy of photon irradiation) with 1.6 Gy per fraction for patients with relapse of breast cancer. Control group established for conventional cytogenetic analysis consisted of 15 healthy persons. Assessment of chromosome aberrations frequency was performed on routinely stained metaphase plates. Lymphocytes from the same patients were analyzed by micronucleus test in combination with fluorescent in situ hybridization (FISH) using pancentromeric DNA probe. Results: Level of chromosome aberrations and micronuclei significantly increased in lymphocytes of patients from both groups during neutron therapy (P < 0.05). This increase was mainly due to chromosome-type aberrations and centromere-negative micronuclei. The prevalent types of aberrations are in agreement with theoretical mechanisms of neutron effects on cells. Conclusion: Cytogenetic effects of fast neutron therapy in lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer were observed. A positive dynamics of radiation-induced chromosomal damages formation during the course was denoted in lymphocytes of cancer patients in both groups.
The study is supported by the state contract № 16.512.11.2063 of Federal Target Research and Technology Program of the Ministry of Education and Science of Russian Federation, Federal Target Research Program “Scientific and scientific-educational personnel of innovative Russia (2009–2013)” (grant № 14.132.21.1319) and grant for young scientists of “OPTEC” company.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer
spellingShingle Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer
Melnikov, A.A.
Vasilyev, S.A.
Musabaeva, L.I.
Velikaya, V.V.
Gribova, O.V.
Startseva, Zh.A.
Urazova, L.N.
Lebedev, I.N.
Choynzonov, E.L.
Original contributions
title_short Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer
title_full Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer
title_fullStr Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer
title_full_unstemmed Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer
title_sort cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer
author Melnikov, A.A.
Vasilyev, S.A.
Musabaeva, L.I.
Velikaya, V.V.
Gribova, O.V.
Startseva, Zh.A.
Urazova, L.N.
Lebedev, I.N.
Choynzonov, E.L.
author_facet Melnikov, A.A.
Vasilyev, S.A.
Musabaeva, L.I.
Velikaya, V.V.
Gribova, O.V.
Startseva, Zh.A.
Urazova, L.N.
Lebedev, I.N.
Choynzonov, E.L.
topic Original contributions
topic_facet Original contributions
publishDate 2012
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim:To assess the frequency and spectrum of chromosome aberrations and micronuclei in peripheral blood lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer during the course of neutron therapy. Materials and Methods: Samples of peripheral blood were obtained from 9 patients with parotid salivary gland tumors (T3N0–3M0) and 8 patients with relapse of breast cancer before, after first fraction and at the end of neutron therapy. The treatment course specified 5.5–8.4 Gy (equivalent to 23–44 Gy of photon irradiation) with 1.3–2.2 Gy per fraction for patients with parotid salivary gland tumors and 4,8–8.0 Gy (equivalent to 30–40 Gy of photon irradiation) with 1.6 Gy per fraction for patients with relapse of breast cancer. Control group established for conventional cytogenetic analysis consisted of 15 healthy persons. Assessment of chromosome aberrations frequency was performed on routinely stained metaphase plates. Lymphocytes from the same patients were analyzed by micronucleus test in combination with fluorescent in situ hybridization (FISH) using pancentromeric DNA probe. Results: Level of chromosome aberrations and micronuclei significantly increased in lymphocytes of patients from both groups during neutron therapy (P < 0.05). This increase was mainly due to chromosome-type aberrations and centromere-negative micronuclei. The prevalent types of aberrations are in agreement with theoretical mechanisms of neutron effects on cells. Conclusion: Cytogenetic effects of fast neutron therapy in lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer were observed. A positive dynamics of radiation-induced chromosomal damages formation during the course was denoted in lymphocytes of cancer patients in both groups.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/139878
citation_txt Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer / A.A. Melnikov, S.A. Vasilyev, L.I. Musabaeva, V.V. Velikaya, O.V. Gribova, Zh.A. Startseva, L.N. Urazova, I.N. Lebedev, E.L. Choynzonov // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 354-357. — Бібліогр.: 19 назв. — англ.
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first_indexed 2025-12-01T03:37:42Z
last_indexed 2025-12-01T03:37:42Z
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