Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer
Aim:To assess the frequency and spectrum of chromosome aberrations and micronuclei in peripheral blood lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer during the course of neutron therapy. Materials and Methods: Samples of peripheral blood were obtained from 9...
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| Published in: | Experimental Oncology |
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| Date: | 2012 |
| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2012
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/139878 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer / A.A. Melnikov, S.A. Vasilyev, L.I. Musabaeva, V.V. Velikaya, O.V. Gribova, Zh.A. Startseva, L.N. Urazova, I.N. Lebedev, E.L. Choynzonov // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 354-357. — Бібліогр.: 19 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862640653697024000 |
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| author | Melnikov, A.A. Vasilyev, S.A. Musabaeva, L.I. Velikaya, V.V. Gribova, O.V. Startseva, Zh.A. Urazova, L.N. Lebedev, I.N. Choynzonov, E.L. |
| author_facet | Melnikov, A.A. Vasilyev, S.A. Musabaeva, L.I. Velikaya, V.V. Gribova, O.V. Startseva, Zh.A. Urazova, L.N. Lebedev, I.N. Choynzonov, E.L. |
| citation_txt | Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer / A.A. Melnikov, S.A. Vasilyev, L.I. Musabaeva, V.V. Velikaya, O.V. Gribova, Zh.A. Startseva, L.N. Urazova, I.N. Lebedev, E.L. Choynzonov // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 354-357. — Бібліогр.: 19 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim:To assess the frequency and spectrum of chromosome aberrations and micronuclei in peripheral blood lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer during the course of neutron therapy. Materials and Methods: Samples of peripheral blood were obtained from 9 patients with parotid salivary gland tumors (T3N0–3M0) and 8 patients with relapse of breast cancer before, after first fraction and at the end of neutron therapy. The treatment course specified 5.5–8.4 Gy (equivalent to 23–44 Gy of photon irradiation) with 1.3–2.2 Gy per fraction for patients with parotid salivary gland tumors and 4,8–8.0 Gy (equivalent to 30–40 Gy of photon irradiation) with 1.6 Gy per fraction for patients with relapse of breast cancer. Control group established for conventional cytogenetic analysis consisted of 15 healthy persons. Assessment of chromosome aberrations frequency was performed on routinely stained metaphase plates. Lymphocytes from the same patients were analyzed by micronucleus test in combination with fluorescent in situ hybridization (FISH) using pancentromeric DNA probe. Results: Level of chromosome aberrations and micronuclei significantly increased in lymphocytes of patients from both groups during neutron therapy (P < 0.05). This increase was mainly due to chromosome-type aberrations and centromere-negative micronuclei. The prevalent types of aberrations are in agreement with theoretical mechanisms of neutron effects on cells. Conclusion: Cytogenetic effects of fast neutron therapy in lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer were observed. A positive dynamics of radiation-induced chromosomal damages formation during the course was denoted in lymphocytes of cancer patients in both groups.
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| first_indexed | 2025-12-01T03:37:42Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-139878 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-01T03:37:42Z |
| publishDate | 2012 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Melnikov, A.A. Vasilyev, S.A. Musabaeva, L.I. Velikaya, V.V. Gribova, O.V. Startseva, Zh.A. Urazova, L.N. Lebedev, I.N. Choynzonov, E.L. 2018-06-21T12:59:35Z 2018-06-21T12:59:35Z 2012 Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer / A.A. Melnikov, S.A. Vasilyev, L.I. Musabaeva, V.V. Velikaya, O.V. Gribova, Zh.A. Startseva, L.N. Urazova, I.N. Lebedev, E.L. Choynzonov // Experimental Oncology. — 2012. — Т. 34, № 4. — С. 354-357. — Бібліогр.: 19 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/139878 Aim:To assess the frequency and spectrum of chromosome aberrations and micronuclei in peripheral blood lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer during the course of neutron therapy. Materials and Methods: Samples of peripheral blood were obtained from 9 patients with parotid salivary gland tumors (T3N0–3M0) and 8 patients with relapse of breast cancer before, after first fraction and at the end of neutron therapy. The treatment course specified 5.5–8.4 Gy (equivalent to 23–44 Gy of photon irradiation) with 1.3–2.2 Gy per fraction for patients with parotid salivary gland tumors and 4,8–8.0 Gy (equivalent to 30–40 Gy of photon irradiation) with 1.6 Gy per fraction for patients with relapse of breast cancer. Control group established for conventional cytogenetic analysis consisted of 15 healthy persons. Assessment of chromosome aberrations frequency was performed on routinely stained metaphase plates. Lymphocytes from the same patients were analyzed by micronucleus test in combination with fluorescent in situ hybridization (FISH) using pancentromeric DNA probe. Results: Level of chromosome aberrations and micronuclei significantly increased in lymphocytes of patients from both groups during neutron therapy (P < 0.05). This increase was mainly due to chromosome-type aberrations and centromere-negative micronuclei. The prevalent types of aberrations are in agreement with theoretical mechanisms of neutron effects on cells. Conclusion: Cytogenetic effects of fast neutron therapy in lymphocytes of patients with parotid salivary gland tumors and relapse of breast cancer were observed. A positive dynamics of radiation-induced chromosomal damages formation during the course was denoted in lymphocytes of cancer patients in both groups. The study is supported by the state contract
 № 16.512.11.2063 of Federal Target Research and
 Technology Program of the Ministry of Education and
 Science of Russian Federation, Federal Target Research
 Program “Scientific and scientific-educational
 personnel of innovative Russia (2009–2013)” (grant
 № 14.132.21.1319) and grant for young scientists of
 “OPTEC” company. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer Article published earlier |
| spellingShingle | Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer Melnikov, A.A. Vasilyev, S.A. Musabaeva, L.I. Velikaya, V.V. Gribova, O.V. Startseva, Zh.A. Urazova, L.N. Lebedev, I.N. Choynzonov, E.L. Original contributions |
| title | Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer |
| title_full | Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer |
| title_fullStr | Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer |
| title_full_unstemmed | Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer |
| title_short | Cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer |
| title_sort | cytogenetic effects of neutron therapy in patients with parotid gland tumors and relapse of breast cancer |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/139878 |
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