Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus

Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus

Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lu...

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Published in:Experimental Oncology
Date:2016
Main Authors: Lykhova, O., Kovalova, O., Bezdenezhnykh, N., Adamenko, I., Vorontsova, A., Strokovska, L., Kudryavets, Yu.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2016
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/140132
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-140132
record_format dspace
spelling Lykhova, O.
Kovalova, O.
Bezdenezhnykh, N.
Adamenko, I.
Vorontsova, A.
Strokovska, L.
Kudryavets, Yu.
2018-06-22T16:38:14Z
2018-06-22T16:38:14Z
2016
Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/140132
Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector. Biological characterlstics of the LL cells were studied with the use of standard cell culture method, cytogenetic and immunocytochemical assays. Resutes: Recombinant baculmirus-mediated transduction of LL cells with ifn-β gene resulted in siguficant decrease of cell growth rate and density both in complete and serum-foee medium. Also. LL cells transduction with ifn-β gene significantly inhfltited cell migration in vitro. Transduction of LL cells by baculovirus vector with or withoot ifn-β gene caused significant genotoxic effect in these cells. Furthermore. ifn-β gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression of p19ARE (p < 0.01). p21WAFI (p < 0.001). cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription factors of epithelial-mesenchymal transition (EMT) Tmist (p < 0.005) and Slug (p < 0.00 I) expression. Conclusions; Transduction with ifn-β gene of LL cells in recombinant baculovirus resulted in aquirement of less malignant phenotype in vitro and suppressed expression ofproteins associated with EMT.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
spellingShingle Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
Lykhova, O.
Kovalova, O.
Bezdenezhnykh, N.
Adamenko, I.
Vorontsova, A.
Strokovska, L.
Kudryavets, Yu.
Original contributions
title_short Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
title_full Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
title_fullStr Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
title_full_unstemmed Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
title_sort inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
author Lykhova, O.
Kovalova, O.
Bezdenezhnykh, N.
Adamenko, I.
Vorontsova, A.
Strokovska, L.
Kudryavets, Yu.
author_facet Lykhova, O.
Kovalova, O.
Bezdenezhnykh, N.
Adamenko, I.
Vorontsova, A.
Strokovska, L.
Kudryavets, Yu.
topic Original contributions
topic_facet Original contributions
publishDate 2016
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector. Biological characterlstics of the LL cells were studied with the use of standard cell culture method, cytogenetic and immunocytochemical assays. Resutes: Recombinant baculmirus-mediated transduction of LL cells with ifn-β gene resulted in siguficant decrease of cell growth rate and density both in complete and serum-foee medium. Also. LL cells transduction with ifn-β gene significantly inhfltited cell migration in vitro. Transduction of LL cells by baculovirus vector with or withoot ifn-β gene caused significant genotoxic effect in these cells. Furthermore. ifn-β gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression of p19ARE (p < 0.01). p21WAFI (p < 0.001). cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription factors of epithelial-mesenchymal transition (EMT) Tmist (p < 0.005) and Slug (p < 0.00 I) expression. Conclusions; Transduction with ifn-β gene of LL cells in recombinant baculovirus resulted in aquirement of less malignant phenotype in vitro and suppressed expression ofproteins associated with EMT.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/140132
citation_txt Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ.
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AT bezdenezhnykhn inhibitionofmalignantpotentialandexpressionofproteinsassociatedwithepithelialmesenchymaltransitioninlewislungcarcinomacellstransducedwithmurineifnβgeneinrecombinantbaculovirus
AT adamenkoi inhibitionofmalignantpotentialandexpressionofproteinsassociatedwithepithelialmesenchymaltransitioninlewislungcarcinomacellstransducedwithmurineifnβgeneinrecombinantbaculovirus
AT vorontsovaa inhibitionofmalignantpotentialandexpressionofproteinsassociatedwithepithelialmesenchymaltransitioninlewislungcarcinomacellstransducedwithmurineifnβgeneinrecombinantbaculovirus
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first_indexed 2025-12-07T18:39:25Z
last_indexed 2025-12-07T18:39:25Z
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