Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal
 transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on...
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| Published in: | Experimental Oncology |
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| Date: | 2016 |
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| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2016
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/140132 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1860248175854485504 |
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| author | Lykhova, O. Kovalova, O. Bezdenezhnykh, N. Adamenko, I. Vorontsova, A. Strokovska, L. Kudryavets, Yu. |
| author_facet | Lykhova, O. Kovalova, O. Bezdenezhnykh, N. Adamenko, I. Vorontsova, A. Strokovska, L. Kudryavets, Yu. |
| citation_txt | Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal
transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector.
Biological characterlstics of the LL cells were studied with the use of standard cell culture method, cytogenetic and immunocytochemical assays. Resutes: Recombinant baculmirus-mediated transduction of LL cells with ifn-β gene resulted in siguficant decrease
of cell growth rate and density both in complete and serum-foee medium. Also. LL cells transduction with ifn-β gene significantly
inhfltited cell migration in vitro. Transduction of LL cells by baculovirus vector with or withoot ifn-β gene caused significant genotoxic effect in these cells. Furthermore. ifn-β gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression of p19ARE (p < 0.01). p21WAFI (p < 0.001). cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription
factors of epithelial-mesenchymal transition (EMT) Tmist (p < 0.005) and Slug (p < 0.00 I) expression. Conclusions; Transduction
with ifn-β gene of LL cells in recombinant baculovirus resulted in aquirement of less malignant phenotype in vitro and suppressed
expression ofproteins associated with EMT.
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| first_indexed | 2025-12-07T18:39:25Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-140132 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T18:39:25Z |
| publishDate | 2016 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Lykhova, O. Kovalova, O. Bezdenezhnykh, N. Adamenko, I. Vorontsova, A. Strokovska, L. Kudryavets, Yu. 2018-06-22T16:38:14Z 2018-06-22T16:38:14Z 2016 Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/140132 Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal
 transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector.
 Biological characterlstics of the LL cells were studied with the use of standard cell culture method, cytogenetic and immunocytochemical assays. Resutes: Recombinant baculmirus-mediated transduction of LL cells with ifn-β gene resulted in siguficant decrease
 of cell growth rate and density both in complete and serum-foee medium. Also. LL cells transduction with ifn-β gene significantly
 inhfltited cell migration in vitro. Transduction of LL cells by baculovirus vector with or withoot ifn-β gene caused significant genotoxic effect in these cells. Furthermore. ifn-β gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression of p19ARE (p < 0.01). p21WAFI (p < 0.001). cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription
 factors of epithelial-mesenchymal transition (EMT) Tmist (p < 0.005) and Slug (p < 0.00 I) expression. Conclusions; Transduction
 with ifn-β gene of LL cells in recombinant baculovirus resulted in aquirement of less malignant phenotype in vitro and suppressed
 expression ofproteins associated with EMT. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus Article published earlier |
| spellingShingle | Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus Lykhova, O. Kovalova, O. Bezdenezhnykh, N. Adamenko, I. Vorontsova, A. Strokovska, L. Kudryavets, Yu. Original contributions |
| title | Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_full | Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_fullStr | Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_full_unstemmed | Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_short | Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_sort | inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/140132 |
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