TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells

TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells

Aim: Oxygen deprivation leading to hypoxia represents a common feature of advanced solid tumors, able to control several aspects of tumor progression. Indeed, ability to respond to changes in oxygen partial pressure represents a hallmark of malignant cells. Aim of this study is to disclose new pathw...

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Published in:Experimental Oncology
Date:2013
Main Authors: Marconi C., Peppicelli S., Bianchini F., Calorini L.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2013
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/145228
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells / C. Marconi, S. Peppicelli, F. Bianchini, L. Calorini // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 187-191. — Бібліогр.: 24 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145228
record_format dspace
spelling Marconi C.
Peppicelli S.
Bianchini F.
Calorini L.
2019-01-19T16:38:55Z
2019-01-19T16:38:55Z
2013
TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells / C. Marconi, S. Peppicelli, F. Bianchini, L. Calorini // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 187-191. — Бібліогр.: 24 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145228
Aim: Oxygen deprivation leading to hypoxia represents a common feature of advanced solid tumors, able to control several aspects of tumor progression. Indeed, ability to respond to changes in oxygen partial pressure represents a hallmark of malignant cells. Aim of this study is to disclose new pathway of hypoxia-induced tumor cell invasion. Materials and Methods: Hs294T human melanoma cells were grown in a gas mixture containing 0.3 % , and used to evaluate invasion on Matrigel-coated polycarbonate filters mounted in Boyden's chambers, MMP release and expression of inflammatory receptors and their ligands. Results: We demonstrate that hypoxia promotes the expression of TNFa receptor 1 (TNFαR1) able to drive a higher ability to penetrate Matrigel-coated filters of Hs294T human melanoma cells, an effect does not mediated by hypoxia-inducible factor (HIF)-1α. Conclusion: Expression inflammatory cytokine receptors in hypoxic human melanoma cells might provide a new target for improving strategies against local and distant tumor cell diffusion. Key Words: TNFa receptor 1, Hs294T human melanoma cells, hypoxia, cell invasiveness, metalloproteinase 2 and 9.
This work was supported by grants from Ente Cassa di Risparmio di Firenze (ECRF) and Instituto Toscano Tumori (ITT).
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
spellingShingle TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
Marconi C.
Peppicelli S.
Bianchini F.
Calorini L.
Original contributions
title_short TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
title_full TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
title_fullStr TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
title_full_unstemmed TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
title_sort tnfα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells
author Marconi C.
Peppicelli S.
Bianchini F.
Calorini L.
author_facet Marconi C.
Peppicelli S.
Bianchini F.
Calorini L.
topic Original contributions
topic_facet Original contributions
publishDate 2013
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: Oxygen deprivation leading to hypoxia represents a common feature of advanced solid tumors, able to control several aspects of tumor progression. Indeed, ability to respond to changes in oxygen partial pressure represents a hallmark of malignant cells. Aim of this study is to disclose new pathway of hypoxia-induced tumor cell invasion. Materials and Methods: Hs294T human melanoma cells were grown in a gas mixture containing 0.3 % , and used to evaluate invasion on Matrigel-coated polycarbonate filters mounted in Boyden's chambers, MMP release and expression of inflammatory receptors and their ligands. Results: We demonstrate that hypoxia promotes the expression of TNFa receptor 1 (TNFαR1) able to drive a higher ability to penetrate Matrigel-coated filters of Hs294T human melanoma cells, an effect does not mediated by hypoxia-inducible factor (HIF)-1α. Conclusion: Expression inflammatory cytokine receptors in hypoxic human melanoma cells might provide a new target for improving strategies against local and distant tumor cell diffusion. Key Words: TNFa receptor 1, Hs294T human melanoma cells, hypoxia, cell invasiveness, metalloproteinase 2 and 9.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145228
fulltext
citation_txt TNFα receptor1 drives hypoxia-promoted invasiveness of human melanoma cells / C. Marconi, S. Peppicelli, F. Bianchini, L. Calorini // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 187-191. — Бібліогр.: 24 назв. — англ.
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AT calorinil tnfαreceptor1driveshypoxiapromotedinvasivenessofhumanmelanomacells
first_indexed 2025-11-24T04:37:37Z
last_indexed 2025-11-24T04:37:37Z
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