Overproduction of free radical species in embryonal cells exposed to low intensity radiofrequency radiation

• Veröffentlicht in: Experimental Oncology
• Datum: 2013
• Hauptverfasser: Burlaka, A., Tsybulin, O., Sidorik, E., Lukin, S., Polishuk, V., Tsehmistrenko, S., Yakymenko, I.
• Format: Artikel
• Sprache: Englisch
• Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2013
• Schlagworte: Original contributions
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/145241
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Overproduction of free radical species in embryonal cells exposed to low intensity radiofrequency radiation / A. Burlaka, O. Tsybulin, E. Sidorik, S. Lukin, V. Polishuk, S. Tsehmistrenko, I. Yakymenko // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 219-225. — Бібліогр.: 60 назв. — англ.

Aim: Long-term exposure of humans to low intensity radiofrequency electromagnetic radiation (RF-EMR) leads to a statistically
 significant increase in tumor incidence. Mechanisms of such the effects are unclear, but features of oxidative stress in living cells
 under RF-EMR exposure were previously reported. Our study aims to assess a production of initial free radical species, which lead
 to oxidative stress in the cell. Materials and Methods: Embryos of Japanese quails were exposed in ovo to extremely low intensity
 RF-EMR of GSM 900 MHz (0.25 µW/cm2
 ) during 158–360 h discontinuously (48 c — ON, 12 c — OFF) before and in the
 initial stages of development. The levels of superoxide (O2·
 -
 ), nitrogen oxide (NO·), thiobarbituric acid reactive substances (TBARS),
 8-oxo-2’-deoxyguanosine (8-oxo-dG) and antioxidant enzymes’ activities were assessed in cells/tissues of 38-h, 5- and 10-day
 RF-EMR exposed and unexposed embryos. Results: The exposure resulted in a significant persistent overproduction of superoxide
 and nitrogen oxide in embryo cells during all period of analyses. As a result, significantly increased levels of TBARS and 8-oxodG
 followed by significantly decreased levels of superoxide dismutase and catalase activities were developed in the exposed embryo
 cells. Conclusion: Exposure of developing quail embryos to extremely low intensity RF-EMR of GSM 900 MHz during at least
 one hundred and fifty-eight hours leads to a significant overproduction of free radicals/reactive oxygen species and oxidative damage
 of DNA in embryo cells. These oxidative changes may lead to pathologies up to oncogenic transformation of cells