Evaluation of serum levels of multiple cytokines and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia using biochip array technology

• Published in: Experimental Oncology
• Date: 2013
• Main Authors: Horacek, J.M., Kupsa, T., Vasatova, M., Jebavy, L., Zak, P.
• Format: Article
• Language: English
• Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2013
• Subjects: Short communications
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/145243
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Evaluation of serum levels of multiple cytokines and adhesion molecules in patients with newly diagnosed acute lymphoblastic leukemia using biochip array technology / J.M. Horacek, T. Kupsa, M. Vasatova, L. Jebavy, P. Zak // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 229-230. — Бібліогр.: 19 назв. — англ.

Aim: Evaluation of serum levels of 17 cytokines and 5 adhesion molecules in patients with acute lymphoblastic leukemia (ALL) and in healthy subjects using biochip array technology. This approach allows multi-analytical determination from a single sample. Methods: A total of 15 ALL patients and 15 healthy subjects (blood donors) were studied. Serum samples were analyzed by biochip based immunoassays on the Evidence Investigator analyzer. T-tests were used for statistical analysis. Results: Comparing cytokine and adhesion molecule levels in ALL patients and in healthy subject, we found significant increase in serum VCAM-1 (p < 0.000001), ICAM-1 (p < 0.0001), L-selectin (p < 0.0001), IL-8 (p < 0.001), MCP-1 (p < 0.01), and significant decrease (p < 0.01) in serum IL-3 and IL-4. Conclusion: Our results indicate that serum levels of specific cytokines and adhesion molecules (VCAM-1, ICAM-1, L-selectin, IL-8, IL-3, IL-4, MCP-1) are significantly altered in patients with newly diagnosed ALL, reflecting acti­vity of the disease. Further investigation is needed to establish if these alterations could be used as a prognostic indicator for ALL.