Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells

Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells

Aim: The purpose of the present study is to evaluate the potent growth inhibitory effects of aqueous wheatgrass extract (AWE) alone and in combination with cisplatin on human breast and cervical cancer cells. Materials and Methods: The cytotoxic potential of AWE alone and in combination with cisplat...

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Published in:Experimental Oncology
Date:2014
Main Authors: Hussain, A., Gheewala, T.M., Vas, A.J., Shah, K., Goala, P., Khan, S., Hinduja, S., Sharma, C.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2014
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/145312
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells / A. Hussain, T.M. Gheewala, A.J. Vas, K. Shah, P. Goala, S. Khan, S. Hinduja, C. Sharma // Experimental Oncology. — 2014. — Т. 36, № 1. — С. 9-16. — Бібліогр.: 57 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145312
record_format dspace
spelling Hussain, A.
Gheewala, T.M.
Vas, A.J.
Shah, K.
Goala, P.
Khan, S.
Hinduja, S.
Sharma, C.
2019-01-20T10:09:20Z
2019-01-20T10:09:20Z
2014
Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells / A. Hussain, T.M. Gheewala, A.J. Vas, K. Shah, P. Goala, S. Khan, S. Hinduja, C. Sharma // Experimental Oncology. — 2014. — Т. 36, № 1. — С. 9-16. — Бібліогр.: 57 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145312
Aim: The purpose of the present study is to evaluate the potent growth inhibitory effects of aqueous wheatgrass extract (AWE) alone and in combination with cisplatin on human breast and cervical cancer cells. Materials and Methods: The cytotoxic potential of AWE alone and in combination with cisplatin was evaluated on human breast and cervical cancer cells (MCF-7 and HeLa) by cell viability assay. Further, the mode of cell death induced by AWE was determined by nuclear morphological examination and cell cycle analysis. These effects were then correlated with the expression of genes involved in apoptosis and proliferation (cyclin D1 and Bax) by RT-PCR. Results: AWE showed dose- and time dependent selective cytotoxicity towards the cancerhighlighting its safe profile. Lower dose combinations of AWE and cisplatin induced increased growth inhibition compared with the individual drugs on both cell lines (combination index < 1) indicating strong synergistic interactions. AWE was found to induce apoptosis and arrested the cells at G0–G1 phase of the cell cycle which correlated with the modulation of expression of bax and cyclin D1 in a time-dependent manner in MCF-7 and HeLa cells. Conclusion: These results suggest that the anti-cancer potential of AWE may be due to apoptosis induction and its anti-proliferative properties. This study also provides the first evidence demonstrating synergism between AWE and cisplatin, which may enhance the therapeutic index of prevention and/or treatment of human breast and cervical cancer. Key Words: Bax, cisplatin, combination, cyclin D1, synergistic, wheatgrass.
The authors would like to thanks to Dr. Tahir Rizvi, UAE University, Al-Ain, UAE for providing the cell lines used in this study.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells
spellingShingle Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells
Hussain, A.
Gheewala, T.M.
Vas, A.J.
Shah, K.
Goala, P.
Khan, S.
Hinduja, S.
Sharma, C.
Original contributions
title_short Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells
title_full Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells
title_fullStr Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells
title_full_unstemmed Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells
title_sort growth inhibitory and adjuvant therapeutic potential of aqueous extract of triticum aestivum on mcf-7 and hela cells
author Hussain, A.
Gheewala, T.M.
Vas, A.J.
Shah, K.
Goala, P.
Khan, S.
Hinduja, S.
Sharma, C.
author_facet Hussain, A.
Gheewala, T.M.
Vas, A.J.
Shah, K.
Goala, P.
Khan, S.
Hinduja, S.
Sharma, C.
topic Original contributions
topic_facet Original contributions
publishDate 2014
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: The purpose of the present study is to evaluate the potent growth inhibitory effects of aqueous wheatgrass extract (AWE) alone and in combination with cisplatin on human breast and cervical cancer cells. Materials and Methods: The cytotoxic potential of AWE alone and in combination with cisplatin was evaluated on human breast and cervical cancer cells (MCF-7 and HeLa) by cell viability assay. Further, the mode of cell death induced by AWE was determined by nuclear morphological examination and cell cycle analysis. These effects were then correlated with the expression of genes involved in apoptosis and proliferation (cyclin D1 and Bax) by RT-PCR. Results: AWE showed dose- and time dependent selective cytotoxicity towards the cancerhighlighting its safe profile. Lower dose combinations of AWE and cisplatin induced increased growth inhibition compared with the individual drugs on both cell lines (combination index < 1) indicating strong synergistic interactions. AWE was found to induce apoptosis and arrested the cells at G0–G1 phase of the cell cycle which correlated with the modulation of expression of bax and cyclin D1 in a time-dependent manner in MCF-7 and HeLa cells. Conclusion: These results suggest that the anti-cancer potential of AWE may be due to apoptosis induction and its anti-proliferative properties. This study also provides the first evidence demonstrating synergism between AWE and cisplatin, which may enhance the therapeutic index of prevention and/or treatment of human breast and cervical cancer. Key Words: Bax, cisplatin, combination, cyclin D1, synergistic, wheatgrass.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145312
citation_txt Growth inhibitory and adjuvant therapeutic potential of aqueous extract of Triticum aestivum on MCF-7 and HeLa cells / A. Hussain, T.M. Gheewala, A.J. Vas, K. Shah, P. Goala, S. Khan, S. Hinduja, C. Sharma // Experimental Oncology. — 2014. — Т. 36, № 1. — С. 9-16. — Бібліогр.: 57 назв. — англ.
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first_indexed 2025-12-07T17:02:18Z
last_indexed 2025-12-07T17:02:18Z
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