Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma

Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma

Aim: Experimental and clinical studies showed that bikunin, a Kunitz-type protease inhibitor, found in urine and amniotic fluid has a role in spread of tumor cells by providing a significant reduction in the levels of urokinase-type plasminogen activator (uPA) and its specific receptor urokinase-typ...

Full description

Saved in:
Bibliographic Details
Published in:Experimental Oncology
Date:2014
Main Authors: Bayraktar, E., Igci, M., Erturhan, S., Igci, Y.Z., Karakok, M., Gogebakan, B., Ulasli, M., Cakmak, E.A., Arslan, A.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2014
Subjects:
Original contributions
Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/145340
Tags: Add Tag
No Tags, Be the first to tag this record!
Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma / E. Bayraktar, M. Igci, S. Erturhan, Y.Z. Igci, M. Karakok, B. Gogebakan, M. Ulasli, E.A. Cakmak, A. Arslan // Experimental Oncology. — 2014. — Т. 36, № 2. — С. 107-111. — Бібліогр.: 43 назв. — англ.

Institution

Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145340
record_format dspace
spelling Bayraktar, E.
Igci, M.
Erturhan, S.
Igci, Y.Z.
Karakok, M.
Gogebakan, B.
Ulasli, M.
Cakmak, E.A.
Arslan, A.
2019-01-20T16:44:32Z
2019-01-20T16:44:32Z
2014
Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma / E. Bayraktar, M. Igci, S. Erturhan, Y.Z. Igci, M. Karakok, B. Gogebakan, M. Ulasli, E.A. Cakmak, A. Arslan // Experimental Oncology. — 2014. — Т. 36, № 2. — С. 107-111. — Бібліогр.: 43 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145340
Aim: Experimental and clinical studies showed that bikunin, a Kunitz-type protease inhibitor, found in urine and amniotic fluid has a role in spread of tumor cells by providing a significant reduction in the levels of urokinase-type plasminogen activator (uPA) and its specific receptor urokinase-type plasminogen activator receptor (uPAR). The aim of this study was to investigate expression of bikunin at the mRNA level and screen for mutations in exon sequence in renal cell carcinoma (RCC) tissues. Materials and Methods: Total RNA and DNA were extracted from paired normal and tumor tissues of total 50 RCC (11 papillary, 8 chromophobe, 26 clear cell, and 5 other types) patients (23 females, mean age: 53.55 ± 14.17; 27 males mean age: 62.1 ± 7.92). Bikunin mRNA levels were detected using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Mutational screening was performed by using single strand conformation polymorphism (SSCP) method and nucleotide sequence analysis. Results: There was a statistically significant decrease in the 25 (50%) of tumor tissues comparing to normal tissues in terms of mRNA levels of bikunin (Wilcoxon signed rank test, p = 0.0337). According to the classification based on subtypes of RCC; clear cell RCC samples displayed a reduced gene expression (p = 0.0148). Additionally, the patients with the age above 50 had low bikunin expression. The SNP rs80057939 spanning 4th exon of bikunin was detected in 13 tumor tissues. However, it was not statistically significant (p > 0.05). Conclusion: Decreased bikunin mRNA level in renal cells might be associated with poor prognosis of renal carcinoma. Therefore, gene constructs or exogenous administration of bikunin might be a potential adjuvant therapy for RCC treatment. Key Words: Bikunin, nucleotide sequence analysis, prognostic marker, renal cell carcinoma, semi-quantitative RT-PCR.
We would like to thank to Assoc. Prof. Seval Kul, Head of the Biostatistics Department, for her help in statistical evaluations.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma
spellingShingle Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma
Bayraktar, E.
Igci, M.
Erturhan, S.
Igci, Y.Z.
Karakok, M.
Gogebakan, B.
Ulasli, M.
Cakmak, E.A.
Arslan, A.
Original contributions
title_short Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma
title_full Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma
title_fullStr Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma
title_full_unstemmed Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma
title_sort reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma
author Bayraktar, E.
Igci, M.
Erturhan, S.
Igci, Y.Z.
Karakok, M.
Gogebakan, B.
Ulasli, M.
Cakmak, E.A.
Arslan, A.
author_facet Bayraktar, E.
Igci, M.
Erturhan, S.
Igci, Y.Z.
Karakok, M.
Gogebakan, B.
Ulasli, M.
Cakmak, E.A.
Arslan, A.
topic Original contributions
topic_facet Original contributions
publishDate 2014
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: Experimental and clinical studies showed that bikunin, a Kunitz-type protease inhibitor, found in urine and amniotic fluid has a role in spread of tumor cells by providing a significant reduction in the levels of urokinase-type plasminogen activator (uPA) and its specific receptor urokinase-type plasminogen activator receptor (uPAR). The aim of this study was to investigate expression of bikunin at the mRNA level and screen for mutations in exon sequence in renal cell carcinoma (RCC) tissues. Materials and Methods: Total RNA and DNA were extracted from paired normal and tumor tissues of total 50 RCC (11 papillary, 8 chromophobe, 26 clear cell, and 5 other types) patients (23 females, mean age: 53.55 ± 14.17; 27 males mean age: 62.1 ± 7.92). Bikunin mRNA levels were detected using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Mutational screening was performed by using single strand conformation polymorphism (SSCP) method and nucleotide sequence analysis. Results: There was a statistically significant decrease in the 25 (50%) of tumor tissues comparing to normal tissues in terms of mRNA levels of bikunin (Wilcoxon signed rank test, p = 0.0337). According to the classification based on subtypes of RCC; clear cell RCC samples displayed a reduced gene expression (p = 0.0148). Additionally, the patients with the age above 50 had low bikunin expression. The SNP rs80057939 spanning 4th exon of bikunin was detected in 13 tumor tissues. However, it was not statistically significant (p > 0.05). Conclusion: Decreased bikunin mRNA level in renal cells might be associated with poor prognosis of renal carcinoma. Therefore, gene constructs or exogenous administration of bikunin might be a potential adjuvant therapy for RCC treatment. Key Words: Bikunin, nucleotide sequence analysis, prognostic marker, renal cell carcinoma, semi-quantitative RT-PCR.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145340
citation_txt Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma / E. Bayraktar, M. Igci, S. Erturhan, Y.Z. Igci, M. Karakok, B. Gogebakan, M. Ulasli, E.A. Cakmak, A. Arslan // Experimental Oncology. — 2014. — Т. 36, № 2. — С. 107-111. — Бібліогр.: 43 назв. — англ.
work_keys_str_mv AT bayraktare reducedgeneexpressionofbikuninasaprognosticmarkerforrenalcellcarcinoma
AT igcim reducedgeneexpressionofbikuninasaprognosticmarkerforrenalcellcarcinoma
AT erturhans reducedgeneexpressionofbikuninasaprognosticmarkerforrenalcellcarcinoma
AT igciyz reducedgeneexpressionofbikuninasaprognosticmarkerforrenalcellcarcinoma
AT karakokm reducedgeneexpressionofbikuninasaprognosticmarkerforrenalcellcarcinoma
AT gogebakanb reducedgeneexpressionofbikuninasaprognosticmarkerforrenalcellcarcinoma
AT ulaslim reducedgeneexpressionofbikuninasaprognosticmarkerforrenalcellcarcinoma
AT cakmakea reducedgeneexpressionofbikuninasaprognosticmarkerforrenalcellcarcinoma
AT arslana reducedgeneexpressionofbikuninasaprognosticmarkerforrenalcellcarcinoma
first_indexed 2025-11-30T10:40:57Z
last_indexed 2025-11-30T10:40:57Z
_version_ 1850857367821877248

Similar Items