Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma
Aim: Experimental and clinical studies showed that bikunin, a Kunitz-type protease inhibitor, found in urine and amniotic fluid has a role in spread of tumor cells by providing a significant reduction in the levels of urokinase-type plasminogen activator (uPA) and its specific receptor urokinase-typ...
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| Published in: | Experimental Oncology |
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| Date: | 2014 |
| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2014
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/145340 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma / E. Bayraktar, M. Igci, S. Erturhan, Y.Z. Igci, M. Karakok, B. Gogebakan, M. Ulasli, E.A. Cakmak, A. Arslan // Experimental Oncology. — 2014. — Т. 36, № 2. — С. 107-111. — Бібліогр.: 43 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862630601126838272 |
|---|---|
| author | Bayraktar, E. Igci, M. Erturhan, S. Igci, Y.Z. Karakok, M. Gogebakan, B. Ulasli, M. Cakmak, E.A. Arslan, A. |
| author_facet | Bayraktar, E. Igci, M. Erturhan, S. Igci, Y.Z. Karakok, M. Gogebakan, B. Ulasli, M. Cakmak, E.A. Arslan, A. |
| citation_txt | Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma / E. Bayraktar, M. Igci, S. Erturhan, Y.Z. Igci, M. Karakok, B. Gogebakan, M. Ulasli, E.A. Cakmak, A. Arslan // Experimental Oncology. — 2014. — Т. 36, № 2. — С. 107-111. — Бібліогр.: 43 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: Experimental and clinical studies showed that bikunin, a Kunitz-type protease inhibitor, found in urine and amniotic fluid has a role in spread of tumor cells by providing a significant reduction in the levels of urokinase-type plasminogen activator (uPA) and its specific receptor urokinase-type plasminogen activator receptor (uPAR). The aim of this study was to investigate expression of bikunin at the mRNA level and screen for mutations in exon sequence in renal cell carcinoma (RCC) tissues. Materials and Methods: Total RNA and DNA were extracted from paired normal and tumor tissues of total 50 RCC (11 papillary, 8 chromophobe, 26 clear cell, and 5 other types) patients (23 females, mean age: 53.55 ± 14.17; 27 males mean age: 62.1 ± 7.92). Bikunin mRNA levels were detected using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Mutational screening was performed by using single strand conformation polymorphism (SSCP) method and nucleotide sequence analysis. Results: There was a statistically significant decrease in the 25 (50%) of tumor tissues comparing to normal tissues in terms of mRNA levels of bikunin (Wilcoxon signed rank test, p = 0.0337). According to the classification based on subtypes of RCC; clear cell RCC samples displayed a reduced gene expression (p = 0.0148). Additionally, the patients with the age above 50 had low bikunin expression. The SNP rs80057939 spanning 4th exon of bikunin was detected in 13 tumor tissues. However, it was not statistically significant (p > 0.05). Conclusion: Decreased bikunin mRNA level in renal cells might be associated with poor prognosis of renal carcinoma. Therefore, gene constructs or exogenous administration of bikunin might be a potential adjuvant therapy for RCC treatment. Key Words: Bikunin, nucleotide sequence analysis, prognostic marker, renal cell carcinoma, semi-quantitative RT-PCR.
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| first_indexed | 2025-11-30T10:40:57Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145340 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-30T10:40:57Z |
| publishDate | 2014 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Bayraktar, E. Igci, M. Erturhan, S. Igci, Y.Z. Karakok, M. Gogebakan, B. Ulasli, M. Cakmak, E.A. Arslan, A. 2019-01-20T16:44:32Z 2019-01-20T16:44:32Z 2014 Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma / E. Bayraktar, M. Igci, S. Erturhan, Y.Z. Igci, M. Karakok, B. Gogebakan, M. Ulasli, E.A. Cakmak, A. Arslan // Experimental Oncology. — 2014. — Т. 36, № 2. — С. 107-111. — Бібліогр.: 43 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145340 Aim: Experimental and clinical studies showed that bikunin, a Kunitz-type protease inhibitor, found in urine and amniotic fluid has a role in spread of tumor cells by providing a significant reduction in the levels of urokinase-type plasminogen activator (uPA) and its specific receptor urokinase-type plasminogen activator receptor (uPAR). The aim of this study was to investigate expression of bikunin at the mRNA level and screen for mutations in exon sequence in renal cell carcinoma (RCC) tissues. Materials and Methods: Total RNA and DNA were extracted from paired normal and tumor tissues of total 50 RCC (11 papillary, 8 chromophobe, 26 clear cell, and 5 other types) patients (23 females, mean age: 53.55 ± 14.17; 27 males mean age: 62.1 ± 7.92). Bikunin mRNA levels were detected using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Mutational screening was performed by using single strand conformation polymorphism (SSCP) method and nucleotide sequence analysis. Results: There was a statistically significant decrease in the 25 (50%) of tumor tissues comparing to normal tissues in terms of mRNA levels of bikunin (Wilcoxon signed rank test, p = 0.0337). According to the classification based on subtypes of RCC; clear cell RCC samples displayed a reduced gene expression (p = 0.0148). Additionally, the patients with the age above 50 had low bikunin expression. The SNP rs80057939 spanning 4th exon of bikunin was detected in 13 tumor tissues. However, it was not statistically significant (p > 0.05). Conclusion: Decreased bikunin mRNA level in renal cells might be associated with poor prognosis of renal carcinoma. Therefore, gene constructs or exogenous administration of bikunin might be a potential adjuvant therapy for RCC treatment. Key Words: Bikunin, nucleotide sequence analysis, prognostic marker, renal cell carcinoma, semi-quantitative RT-PCR. We would like to thank to Assoc. Prof. Seval Kul, Head of the Biostatistics Department, for her help in statistical evaluations. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma Article published earlier |
| spellingShingle | Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma Bayraktar, E. Igci, M. Erturhan, S. Igci, Y.Z. Karakok, M. Gogebakan, B. Ulasli, M. Cakmak, E.A. Arslan, A. Original contributions |
| title | Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma |
| title_full | Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma |
| title_fullStr | Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma |
| title_full_unstemmed | Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma |
| title_short | Reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma |
| title_sort | reduced gene expression of bikunin as a prognostic marker for renal cell carcinoma |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145340 |
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