Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin

Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin

Aim : To study in vivo the peculiarities of changes of iron metabolism and antioxidant system in dynamics of growth of Guerin carcinoma with different sensitivity to cisplatin. Materials and Methods: In order to evaluate the content of metallothionein-1 (MT-1) in tumor homogenates and blood serum of...

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Veröffentlicht in:Experimental Oncology
Datum:2014
Hauptverfasser: Chekhun, V.F., Lozovska, Y.V., Burlaka, A.P., Lukyanova, N.Y., Todor, I.N., Naleskina, L.A.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2014
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/145366
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Zitieren:Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin / V.F. Chekhun, Y.V. Lozovska, A.P. Burlaka, N.Y. Lukyanova, I.N. Todor, L.A. Naleskina // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 196-201. — Бібліогр.: 26 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145366
record_format dspace
spelling Chekhun, V.F.
Lozovska, Y.V.
Burlaka, A.P.
Lukyanova, N.Y.
Todor, I.N.
Naleskina, L.A.
2019-01-21T08:16:18Z
2019-01-21T08:16:18Z
2014
Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin / V.F. Chekhun, Y.V. Lozovska, A.P. Burlaka, N.Y. Lukyanova, I.N. Todor, L.A. Naleskina // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 196-201. — Бібліогр.: 26 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145366
Aim : To study in vivo the peculiarities of changes of iron metabolism and antioxidant system in dynamics of growth of Guerin carcinoma with different sensitivity to cisplatin. Materials and Methods: In order to evaluate the content of metallothionein-1 (MT-1) in tumor homogenates and blood serum of rats with cisplatin-sensitive and cisplatin-resistant Guerin carcinoma the immunoenzyme method was used. The evaluation of ceruloplasmin activity, content of “free iron” complexes, superoxide and NO-generating acti­vity of NADPH-oxidase and iNOS activity in neutrophils, blood serum and tumor homogenates was measured by EPR-spectro­scopy. Results: Maximal accumulation of MT-1 in blood serum and tumor, more pronounced in resistant strain, at the border of latent and exponential phase of growth has been shown that is the evidence of protective role of this protein in the respect to the generation of free radical compounds. It has been determined that in animals with cisplatin-resistant strain of Guerin carcinoma, increase of “free iron” complexes is more apparent both on the level of tumor and organism on the background on increase of CP/TR ratio that is the consequence of organism antioxidant protection system disorder. Conclutions: Mentioned changes in metabolism of iron with its accumulation in tumor and further reprogramming of mitochondria metabolism and activity of NADPH-oxidase for non-transformed cells are favorable conditions for the formation of oxidative phenotype of tumor. Key Words: Guerin carcinoma, metallothionein-1, antioxidant system, transferrin, ceruloplasmin, “free iron” complexes, activity of NADPH-oxidase and iNOS neutrophils.
The study is performed in terms of the topic № 2.2.5.361 “Molecular epidemiology of metal-containing proteins in tumor-host interactions” of the target research program “Functional genomics and metabolomics in system biology” (2012–2016).
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
spellingShingle Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
Chekhun, V.F.
Lozovska, Y.V.
Burlaka, A.P.
Lukyanova, N.Y.
Todor, I.N.
Naleskina, L.A.
Original contributions
title_short Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
title_full Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
title_fullStr Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
title_full_unstemmed Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
title_sort peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
author Chekhun, V.F.
Lozovska, Y.V.
Burlaka, A.P.
Lukyanova, N.Y.
Todor, I.N.
Naleskina, L.A.
author_facet Chekhun, V.F.
Lozovska, Y.V.
Burlaka, A.P.
Lukyanova, N.Y.
Todor, I.N.
Naleskina, L.A.
topic Original contributions
topic_facet Original contributions
publishDate 2014
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim : To study in vivo the peculiarities of changes of iron metabolism and antioxidant system in dynamics of growth of Guerin carcinoma with different sensitivity to cisplatin. Materials and Methods: In order to evaluate the content of metallothionein-1 (MT-1) in tumor homogenates and blood serum of rats with cisplatin-sensitive and cisplatin-resistant Guerin carcinoma the immunoenzyme method was used. The evaluation of ceruloplasmin activity, content of “free iron” complexes, superoxide and NO-generating acti­vity of NADPH-oxidase and iNOS activity in neutrophils, blood serum and tumor homogenates was measured by EPR-spectro­scopy. Results: Maximal accumulation of MT-1 in blood serum and tumor, more pronounced in resistant strain, at the border of latent and exponential phase of growth has been shown that is the evidence of protective role of this protein in the respect to the generation of free radical compounds. It has been determined that in animals with cisplatin-resistant strain of Guerin carcinoma, increase of “free iron” complexes is more apparent both on the level of tumor and organism on the background on increase of CP/TR ratio that is the consequence of organism antioxidant protection system disorder. Conclutions: Mentioned changes in metabolism of iron with its accumulation in tumor and further reprogramming of mitochondria metabolism and activity of NADPH-oxidase for non-transformed cells are favorable conditions for the formation of oxidative phenotype of tumor. Key Words: Guerin carcinoma, metallothionein-1, antioxidant system, transferrin, ceruloplasmin, “free iron” complexes, activity of NADPH-oxidase and iNOS neutrophils.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145366
citation_txt Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin / V.F. Chekhun, Y.V. Lozovska, A.P. Burlaka, N.Y. Lukyanova, I.N. Todor, L.A. Naleskina // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 196-201. — Бібліогр.: 26 назв. — англ.
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first_indexed 2025-11-30T12:16:37Z
last_indexed 2025-11-30T12:16:37Z
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