Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
Aim : To study in vivo the peculiarities of changes of iron metabolism and antioxidant system in dynamics of growth of Guerin carcinoma with different sensitivity to cisplatin. Materials and Methods: In order to evaluate the content of metallothionein-1 (MT-1) in tumor homogenates and blood serum of...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2014 |
| Hauptverfasser: | , , , , , |
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| Sprache: | Englisch |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2014
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| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/145366 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin / V.F. Chekhun, Y.V. Lozovska, A.P. Burlaka, N.Y. Lukyanova, I.N. Todor, L.A. Naleskina // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 196-201. — Бібліогр.: 26 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862632101662162944 |
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| author | Chekhun, V.F. Lozovska, Y.V. Burlaka, A.P. Lukyanova, N.Y. Todor, I.N. Naleskina, L.A. |
| author_facet | Chekhun, V.F. Lozovska, Y.V. Burlaka, A.P. Lukyanova, N.Y. Todor, I.N. Naleskina, L.A. |
| citation_txt | Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin / V.F. Chekhun, Y.V. Lozovska, A.P. Burlaka, N.Y. Lukyanova, I.N. Todor, L.A. Naleskina // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 196-201. — Бібліогр.: 26 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim : To study in vivo the peculiarities of changes of iron metabolism and antioxidant system in dynamics of growth of Guerin carcinoma with different sensitivity to cisplatin. Materials and Methods: In order to evaluate the content of metallothionein-1 (MT-1) in tumor homogenates and blood serum of rats with cisplatin-sensitive and cisplatin-resistant Guerin carcinoma the immunoenzyme method was used. The evaluation of ceruloplasmin activity, content of “free iron” complexes, superoxide and NO-generating activity of NADPH-oxidase and iNOS activity in neutrophils, blood serum and tumor homogenates was measured by EPR-spectroscopy. Results: Maximal accumulation of MT-1 in blood serum and tumor, more pronounced in resistant strain, at the border of latent and exponential phase of growth has been shown that is the evidence of protective role of this protein in the respect to the generation of free radical compounds. It has been determined that in animals with cisplatin-resistant strain of Guerin carcinoma, increase of “free iron” complexes is more apparent both on the level of tumor and organism on the background on increase of CP/TR ratio that is the consequence of organism antioxidant protection system disorder. Conclutions: Mentioned changes in metabolism of iron with its accumulation in tumor and further reprogramming of mitochondria metabolism and activity of NADPH-oxidase for non-transformed cells are favorable conditions for the formation of oxidative phenotype of tumor. Key Words: Guerin carcinoma, metallothionein-1, antioxidant system, transferrin, ceruloplasmin, “free iron” complexes, activity of NADPH-oxidase and iNOS neutrophils.
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| first_indexed | 2025-11-30T12:16:37Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145366 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-30T12:16:37Z |
| publishDate | 2014 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Chekhun, V.F. Lozovska, Y.V. Burlaka, A.P. Lukyanova, N.Y. Todor, I.N. Naleskina, L.A. 2019-01-21T08:16:18Z 2019-01-21T08:16:18Z 2014 Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin / V.F. Chekhun, Y.V. Lozovska, A.P. Burlaka, N.Y. Lukyanova, I.N. Todor, L.A. Naleskina // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 196-201. — Бібліогр.: 26 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145366 Aim : To study in vivo the peculiarities of changes of iron metabolism and antioxidant system in dynamics of growth of Guerin carcinoma with different sensitivity to cisplatin. Materials and Methods: In order to evaluate the content of metallothionein-1 (MT-1) in tumor homogenates and blood serum of rats with cisplatin-sensitive and cisplatin-resistant Guerin carcinoma the immunoenzyme method was used. The evaluation of ceruloplasmin activity, content of “free iron” complexes, superoxide and NO-generating activity of NADPH-oxidase and iNOS activity in neutrophils, blood serum and tumor homogenates was measured by EPR-spectroscopy. Results: Maximal accumulation of MT-1 in blood serum and tumor, more pronounced in resistant strain, at the border of latent and exponential phase of growth has been shown that is the evidence of protective role of this protein in the respect to the generation of free radical compounds. It has been determined that in animals with cisplatin-resistant strain of Guerin carcinoma, increase of “free iron” complexes is more apparent both on the level of tumor and organism on the background on increase of CP/TR ratio that is the consequence of organism antioxidant protection system disorder. Conclutions: Mentioned changes in metabolism of iron with its accumulation in tumor and further reprogramming of mitochondria metabolism and activity of NADPH-oxidase for non-transformed cells are favorable conditions for the formation of oxidative phenotype of tumor. Key Words: Guerin carcinoma, metallothionein-1, antioxidant system, transferrin, ceruloplasmin, “free iron” complexes, activity of NADPH-oxidase and iNOS neutrophils. The study is performed in terms of the topic № 2.2.5.361 “Molecular epidemiology of metal-containing proteins in tumor-host interactions” of the target research program “Functional genomics and metabolomics in system biology” (2012–2016). en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin Article published earlier |
| spellingShingle | Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin Chekhun, V.F. Lozovska, Y.V. Burlaka, A.P. Lukyanova, N.Y. Todor, I.N. Naleskina, L.A. Original contributions |
| title | Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin |
| title_full | Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin |
| title_fullStr | Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin |
| title_full_unstemmed | Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin |
| title_short | Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin |
| title_sort | peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145366 |
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