Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype

Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype

Photodynamic therapy (PDT) is considered as a possible alternative approach to overcoming multidrug resistance (MDR). Analysis of cross-resistance to PDT in cells with different MDR pathways and resistance levels seems to be advantageous for elucidating the general mechanisms of cancer cell resistan...

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Published in:Experimental Oncology
Date:2014
Main Authors: Philchenkov, A.A., Shishko, E.D., Zavelevich, M.P., Kuiava, L.M., Miura, K., Blokhin, D.Y., Shton, I.O., Gamaleia, N.F.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2014
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/145375
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype / A.A. Philchenkov, E.D. Shishko, M.P. Zavelevich, L.M. Kuiava, K. Miura, D.Y. Blokhin, I.O. Shton, N.F. Gamaleia // Experimental Oncology. — 2014. — Т. 36, № 4. — С. 241-245. — Бібліогр.: 25 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145375
record_format dspace
spelling Philchenkov, A.A.
Shishko, E.D.
Zavelevich, M.P.
Kuiava, L.M.
Miura, K.
Blokhin, D.Y.
Shton, I.O.
Gamaleia, N.F.
2019-01-21T08:32:43Z
2019-01-21T08:32:43Z
2014
Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype / A.A. Philchenkov, E.D. Shishko, M.P. Zavelevich, L.M. Kuiava, K. Miura, D.Y. Blokhin, I.O. Shton, N.F. Gamaleia // Experimental Oncology. — 2014. — Т. 36, № 4. — С. 241-245. — Бібліогр.: 25 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145375
Photodynamic therapy (PDT) is considered as a possible alternative approach to overcoming multidrug resistance (MDR). Analysis of cross-resistance to PDT in cells with different MDR pathways and resistance levels seems to be advantageous for elucidating the general mechanisms of cancer cell resistance to various treatment modalities. Aim: The aim of the study was to clarify whether the Jurkat/A4 leukemia cells with MDR phenotype are cross-resistant to PDT. Methods: Human T-cell acute lymphoblastic leukemia line Jurkat and Jurkat/A4 subline with MDR phenotype were used. 5-Aminolevulinic acid (ALA) and Photolon (a complex of chlorine-e6 and polyvinylpyrrolidone; PL) or gold nanocomposite of PL were applied as photosensitizers. The cells were pretreated with photosensitizers and exposed to laser radiation at corresponding wavelengths. The phototoxicity was assessed in trypan blue exclusion test. The hypodiploid cell fraction was analyzed by flow cytometry of propidium iodide-stained cells. Expression of genes related to PDT resistance was analyzed by microarray technique with Affymetrix U133A chips. Results: ALA-mediated PDT resulted in dose-dependent cell death in both lines, the relative photodynamic efficacy in Jurkat/A4 cells being inferior to that in the parental Jurkat cells. There was no correlation between phototoxicity and apoptosis induction both in Jurkat and Jurkat/A4 cells. PL-mediated general phototoxicity in Jurkat cells amounted up to 75% at the maximal photosensitizer dose with about 40% of apoptotic death fraction. PL-phototoxicity in Jurkat/A4 cells was considerably lower. In contrast to Jurkat cells, PL-gold composite did not increase the efficacy of photosensitization as compared to free PL in Jurkat/A4 cells. Conclusions: Multidrug-resistant Jurkat/A4 cells exhibit reduced sensitivity to phototoxic effect in comparison with parental Jurkat cells independently of nature of the photosensitizer being assayed. Key Words: photodynamic therapy, leukemia cells, multidrug resistance, apoptosis.
This work was supported by the grant № 0110U005553 of Complex Interdisciplinary Program for Scientific Research of the NAS of Ukraine “Fundamental problems of nanostructural systems, nanomaterials, nanotechnologies” and grant № F53.4/056 from State Fund for Fundamental Research (DFFD), Ukraine. The ­authors are grateful to Dr. Andrey Mikhailov (Turku Centre for Biotechnology, University of Turku/Abo Akademi University, Turku, Finland) for DNA-microarray analysis.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype
spellingShingle Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype
Philchenkov, A.A.
Shishko, E.D.
Zavelevich, M.P.
Kuiava, L.M.
Miura, K.
Blokhin, D.Y.
Shton, I.O.
Gamaleia, N.F.
Original contributions
title_short Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype
title_full Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype
title_fullStr Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype
title_full_unstemmed Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype
title_sort photodynamic responsiveness of human leukemia jurkat/a4 cells with multidrug resistant phenotype
author Philchenkov, A.A.
Shishko, E.D.
Zavelevich, M.P.
Kuiava, L.M.
Miura, K.
Blokhin, D.Y.
Shton, I.O.
Gamaleia, N.F.
author_facet Philchenkov, A.A.
Shishko, E.D.
Zavelevich, M.P.
Kuiava, L.M.
Miura, K.
Blokhin, D.Y.
Shton, I.O.
Gamaleia, N.F.
topic Original contributions
topic_facet Original contributions
publishDate 2014
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Photodynamic therapy (PDT) is considered as a possible alternative approach to overcoming multidrug resistance (MDR). Analysis of cross-resistance to PDT in cells with different MDR pathways and resistance levels seems to be advantageous for elucidating the general mechanisms of cancer cell resistance to various treatment modalities. Aim: The aim of the study was to clarify whether the Jurkat/A4 leukemia cells with MDR phenotype are cross-resistant to PDT. Methods: Human T-cell acute lymphoblastic leukemia line Jurkat and Jurkat/A4 subline with MDR phenotype were used. 5-Aminolevulinic acid (ALA) and Photolon (a complex of chlorine-e6 and polyvinylpyrrolidone; PL) or gold nanocomposite of PL were applied as photosensitizers. The cells were pretreated with photosensitizers and exposed to laser radiation at corresponding wavelengths. The phototoxicity was assessed in trypan blue exclusion test. The hypodiploid cell fraction was analyzed by flow cytometry of propidium iodide-stained cells. Expression of genes related to PDT resistance was analyzed by microarray technique with Affymetrix U133A chips. Results: ALA-mediated PDT resulted in dose-dependent cell death in both lines, the relative photodynamic efficacy in Jurkat/A4 cells being inferior to that in the parental Jurkat cells. There was no correlation between phototoxicity and apoptosis induction both in Jurkat and Jurkat/A4 cells. PL-mediated general phototoxicity in Jurkat cells amounted up to 75% at the maximal photosensitizer dose with about 40% of apoptotic death fraction. PL-phototoxicity in Jurkat/A4 cells was considerably lower. In contrast to Jurkat cells, PL-gold composite did not increase the efficacy of photosensitization as compared to free PL in Jurkat/A4 cells. Conclusions: Multidrug-resistant Jurkat/A4 cells exhibit reduced sensitivity to phototoxic effect in comparison with parental Jurkat cells independently of nature of the photosensitizer being assayed. Key Words: photodynamic therapy, leukemia cells, multidrug resistance, apoptosis.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145375
citation_txt Photodynamic responsiveness of human leukemia Jurkat/A4 cells with multidrug resistant phenotype / A.A. Philchenkov, E.D. Shishko, M.P. Zavelevich, L.M. Kuiava, K. Miura, D.Y. Blokhin, I.O. Shton, N.F. Gamaleia // Experimental Oncology. — 2014. — Т. 36, № 4. — С. 241-245. — Бібліогр.: 25 назв. — англ.
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first_indexed 2025-11-27T21:26:43Z
last_indexed 2025-11-27T21:26:43Z
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