Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma
Study was aimed to analyze the dynamics of changes and study interrelations between content of ferritin, transferrin, active gelatinases (MMP-2 and -9) in blood serum and tumor tissue, free iron, rate of superoxide radicals generation in tumor, activity of NADPH-oxidase and iNOS in neutrophils rats...
Збережено в:
| Опубліковано в: : | Experimental Oncology |
|---|---|
| Дата: | 2015 |
| Автори: | , , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2015
|
| Теми: | |
| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/145452 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma / A.P. Burlaka, I.I. Ganusevich, Yu.V. Lozovska, N.Yu. Lukianova, V.F. Chekhun // Experimental Oncology. — 2015. — Т. 37, № 1. — С. 36-39. — Бібліогр.: 31 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862541736813789184 |
|---|---|
| author | Burlaka, A.P. Ganusevich, I.I. Lozovska, Yu.V. Lukianova, N.Yu. Chekhun, V.F. |
| author_facet | Burlaka, A.P. Ganusevich, I.I. Lozovska, Yu.V. Lukianova, N.Yu. Chekhun, V.F. |
| citation_txt | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma / A.P. Burlaka, I.I. Ganusevich, Yu.V. Lozovska, N.Yu. Lukianova, V.F. Chekhun // Experimental Oncology. — 2015. — Т. 37, № 1. — С. 36-39. — Бібліогр.: 31 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Study was aimed to analyze the dynamics of changes and study interrelations between content of ferritin, transferrin, active gelatinases (MMP-2 and -9) in blood serum and tumor tissue, free iron, rate of superoxide radicals generation in tumor, activity of NADPH-oxidase and iNOS in neutrophils rats with sensitive and resistant strains of Guerin carcinoma (GC). Materials and Methods: In order to obtain resistant tumor, 12 courses of cisplatin chemotherapy have been carried out on rats bearing GC. Levels of transferrin and free iron were determined by analysis of EPR spectra from computerized radiospectrometer EPR RE-1307 at temperature of liquid nitrogen. Rate of superoxide radicals and nitric oxide generation in tumor and neutrophils of blood was determined by EPR using spin traps at room temperature. Content of ferritin in tumor homogenate and blood serum of rats with GC was determined by ELISA method using corresponding kits. Concentration of active forms of MMP-2 and -9 in obtained samples was determined using method of zymography. Results: Unregulated generation of superoxide radicals and NO by mitochondria of tumor cells and NADPH-oxidase and iNOS neutrophils via oxidation of iron-containing proteins causes the accumulation of “free iron” complexes in blood and tumor tissue of rats able to evoke oxide-induced damages of macromolecules. It has been shown that for resistant strain of carcinoma, as compared with sensitive one, significantly higher concentrations of active forms of MMP-2 and -9 in blood serum of rats are typical. Dynamics of gelatinases activity changes in tumor tissue corresponds in general with dynamics of changes in serum. In tumor tissue of rats the indices of gelatinases activity positively correlate with rate of superoxide radicals generation, content of “free iron” complexes, ferritin and activity of transferrin. Cytostatic agent increased levels of reactive oxygen species (ROS) and self-amplify rate of superoxide radicals generation. In turn, activation of MMPs via superoxide-depending regulation allows tumor cells to facilitate migration, invasion and finally — formation of metastatic centers. Mentioned above tumor “oxide phenotype” determines high level of its aggressiveness and forms corresponding level of drug resistance. Conclusions: Thus, high levels of superoxide radicals oxidize transport proteins and form free iron pool. Iron ions, via Haber — Weiss mechanism, initiate generation of the hydroxyl radicals, which also enhance oxidation processes. Key Words: drug resistance, redox-regulation, iron-containing proteins, matrix metalloproteinases.
|
| first_indexed | 2025-11-24T16:48:07Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145452 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-24T16:48:07Z |
| publishDate | 2015 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Burlaka, A.P. Ganusevich, I.I. Lozovska, Yu.V. Lukianova, N.Yu. Chekhun, V.F. 2019-01-21T21:25:34Z 2019-01-21T21:25:34Z 2015 Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma / A.P. Burlaka, I.I. Ganusevich, Yu.V. Lozovska, N.Yu. Lukianova, V.F. Chekhun // Experimental Oncology. — 2015. — Т. 37, № 1. — С. 36-39. — Бібліогр.: 31 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145452 Study was aimed to analyze the dynamics of changes and study interrelations between content of ferritin, transferrin, active gelatinases (MMP-2 and -9) in blood serum and tumor tissue, free iron, rate of superoxide radicals generation in tumor, activity of NADPH-oxidase and iNOS in neutrophils rats with sensitive and resistant strains of Guerin carcinoma (GC). Materials and Methods: In order to obtain resistant tumor, 12 courses of cisplatin chemotherapy have been carried out on rats bearing GC. Levels of transferrin and free iron were determined by analysis of EPR spectra from computerized radiospectrometer EPR RE-1307 at temperature of liquid nitrogen. Rate of superoxide radicals and nitric oxide generation in tumor and neutrophils of blood was determined by EPR using spin traps at room temperature. Content of ferritin in tumor homogenate and blood serum of rats with GC was determined by ELISA method using corresponding kits. Concentration of active forms of MMP-2 and -9 in obtained samples was determined using method of zymography. Results: Unregulated generation of superoxide radicals and NO by mitochondria of tumor cells and NADPH-oxidase and iNOS neutrophils via oxidation of iron-containing proteins causes the accumulation of “free iron” complexes in blood and tumor tissue of rats able to evoke oxide-induced damages of macromolecules. It has been shown that for resistant strain of carcinoma, as compared with sensitive one, significantly higher concentrations of active forms of MMP-2 and -9 in blood serum of rats are typical. Dynamics of gelatinases activity changes in tumor tissue corresponds in general with dynamics of changes in serum. In tumor tissue of rats the indices of gelatinases activity positively correlate with rate of superoxide radicals generation, content of “free iron” complexes, ferritin and activity of transferrin. Cytostatic agent increased levels of reactive oxygen species (ROS) and self-amplify rate of superoxide radicals generation. In turn, activation of MMPs via superoxide-depending regulation allows tumor cells to facilitate migration, invasion and finally — formation of metastatic centers. Mentioned above tumor “oxide phenotype” determines high level of its aggressiveness and forms corresponding level of drug resistance. Conclusions: Thus, high levels of superoxide radicals oxidize transport proteins and form free iron pool. Iron ions, via Haber — Weiss mechanism, initiate generation of the hydroxyl radicals, which also enhance oxidation processes. Key Words: drug resistance, redox-regulation, iron-containing proteins, matrix metalloproteinases. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma Article published earlier |
| spellingShingle | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma Burlaka, A.P. Ganusevich, I.I. Lozovska, Yu.V. Lukianova, N.Yu. Chekhun, V.F. Original contributions |
| title | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma |
| title_full | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma |
| title_fullStr | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma |
| title_full_unstemmed | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma |
| title_short | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma |
| title_sort | redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant guerin carcinoma |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145452 |
| work_keys_str_mv | AT burlakaap redoxregulationofgelatinasesduringgrowthofcisplatinsensitiveandresistantguerincarcinoma AT ganusevichii redoxregulationofgelatinasesduringgrowthofcisplatinsensitiveandresistantguerincarcinoma AT lozovskayuv redoxregulationofgelatinasesduringgrowthofcisplatinsensitiveandresistantguerincarcinoma AT lukianovanyu redoxregulationofgelatinasesduringgrowthofcisplatinsensitiveandresistantguerincarcinoma AT chekhunvf redoxregulationofgelatinasesduringgrowthofcisplatinsensitiveandresistantguerincarcinoma |