Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell
Aim: In this study, we evaluated the carcinostatic effects of combined ascorbic acid (AsA) and a capacitive-resistive electric transfer (CRet) hyperthermic apparatus-induced hyperthermic treatment on Ehrlich ascites tumor (EAT) cells. Materials and Methods: EAT cells were exposed to various AsA (0–1...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2015 |
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| Sprache: | Englisch |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2015
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell / Y. Saitoh, T. Yoshimoto, S. Kato, N. Miwa // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 94-99. — Бібліогр.: 36 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862643829898739712 |
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| author | Saitoh, Y. Yoshimoto, T. Kato, S. Miwa, N. |
| author_facet | Saitoh, Y. Yoshimoto, T. Kato, S. Miwa, N. |
| citation_txt | Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell / Y. Saitoh, T. Yoshimoto, S. Kato, N. Miwa // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 94-99. — Бібліогр.: 36 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: In this study, we evaluated the carcinostatic effects of combined ascorbic acid (AsA) and a capacitive-resistive electric transfer (CRet) hyperthermic apparatus-induced hyperthermic treatment on Ehrlich ascites tumor (EAT) cells. Materials and Methods: EAT cells were exposed to various AsA (0–10 mM) concentrations for 1 h; they subsequently underwent CRet treatment for 15 min at 42 °C. Cell viability was assessed by the WST-8 assay 24 h after the combined treatment. Reactive oxygen species involvement was evaluated using catalase and tempol; caspase-3/7 activation was determined by their fluorescent substrates; cell proliferation were estimated by time-lapse observation. The effect on the cell cycle was analyzed by flow cytometry. Results: Combined AsA and CRet treatment synergistically suppressed cell viability compared with either treatment alone, and these synergistically carcinostatic effects were evident even at noncytotoxic concentrations of AsA alone (≤ 2 mM). The carcinostatic effects of combined AsA and CRet treatment were attenuated in a dose-dependent manner by catalase addition, but not by the superoxide anion radical scavenger tempol. Time-lapse observation revealed that combined AsA and CRet treatment activated caspase-3/7 at 10–24 h after treatment, accompanied by significant cell growth suppression. Cell cycle analysis revealed that the rate of sub-G1-phase (apoptotic) cells was drastically increased at 12 h and 24 h, and that the G2/M-phase cells gradually increased at 6–24 h after treatment. Conclusion: These results indicate that combined AsA and CRet treatment synergistically inhibits EAT cell growth through G2/M arrest and apoptosis induction via H₂O₂ generation at lower AsA concentrations; this carcinostatic effect cannot be exerted by AsA alone. Key Words: L-ascorbic acid, CRet system, hyperthermic action, Ehrlich ascites tumor cell, carcinostatic effect.
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| first_indexed | 2025-12-01T08:13:06Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-145462 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-01T08:13:06Z |
| publishDate | 2015 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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| spelling | Saitoh, Y. Yoshimoto, T. Kato, S. Miwa, N. 2019-01-22T11:03:13Z 2019-01-22T11:03:13Z 2015 Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell / Y. Saitoh, T. Yoshimoto, S. Kato, N. Miwa // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 94-99. — Бібліогр.: 36 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145462 Aim: In this study, we evaluated the carcinostatic effects of combined ascorbic acid (AsA) and a capacitive-resistive electric transfer (CRet) hyperthermic apparatus-induced hyperthermic treatment on Ehrlich ascites tumor (EAT) cells. Materials and Methods: EAT cells were exposed to various AsA (0–10 mM) concentrations for 1 h; they subsequently underwent CRet treatment for 15 min at 42 °C. Cell viability was assessed by the WST-8 assay 24 h after the combined treatment. Reactive oxygen species involvement was evaluated using catalase and tempol; caspase-3/7 activation was determined by their fluorescent substrates; cell proliferation were estimated by time-lapse observation. The effect on the cell cycle was analyzed by flow cytometry. Results: Combined AsA and CRet treatment synergistically suppressed cell viability compared with either treatment alone, and these synergistically carcinostatic effects were evident even at noncytotoxic concentrations of AsA alone (≤ 2 mM). The carcinostatic effects of combined AsA and CRet treatment were attenuated in a dose-dependent manner by catalase addition, but not by the superoxide anion radical scavenger tempol. Time-lapse observation revealed that combined AsA and CRet treatment activated caspase-3/7 at 10–24 h after treatment, accompanied by significant cell growth suppression. Cell cycle analysis revealed that the rate of sub-G1-phase (apoptotic) cells was drastically increased at 12 h and 24 h, and that the G2/M-phase cells gradually increased at 6–24 h after treatment. Conclusion: These results indicate that combined AsA and CRet treatment synergistically inhibits EAT cell growth through G2/M arrest and apoptosis induction via H₂O₂ generation at lower AsA concentrations; this carcinostatic effect cannot be exerted by AsA alone. Key Words: L-ascorbic acid, CRet system, hyperthermic action, Ehrlich ascites tumor cell, carcinostatic effect. We thank Dr. K. Kageyama and Dr. R. Asada for their technical advice, and thank INDIBA-Japan, Tokyo, for technical assistance. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell Article published earlier |
| spellingShingle | Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell Saitoh, Y. Yoshimoto, T. Kato, S. Miwa, N. Original contributions |
| title | Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell |
| title_full | Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell |
| title_fullStr | Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell |
| title_full_unstemmed | Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell |
| title_short | Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell |
| title_sort | synergic carcinostatic effects of ascorbic acid and hyperthermia on ehrlich ascites tumor cell |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145462 |
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