Assessment of gold nanoparticle effect on prostate cancer LNCaP cells
In recent years gold nanoparticles (AuNPs) have received considerable attention for various biomedical applications including diagnostics and targeted drug delivery. However, more research is still needed to characterize such aspects of their use in clinical oncology as permeability, retention and f...
Saved in:
| Published in: | Experimental Oncology |
|---|---|
| Date: | 2015 |
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2015
|
| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/145463 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Assessment of gold nanoparticle effect on prostate cancer LNCaP cells / A.G. Reznikov, O.A. Salivonyk, A.G. Sotkis, Y.M. Shuba // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 100-104. — Бібліогр.: 27 назв. — англ. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862726242101362688 |
|---|---|
| author | Reznikov, A.G. Salivonyk, O.A. Sotkis, A.G. Shuba, Y.M. |
| author_facet | Reznikov, A.G. Salivonyk, O.A. Sotkis, A.G. Shuba, Y.M. |
| citation_txt | Assessment of gold nanoparticle effect on prostate cancer LNCaP cells / A.G. Reznikov, O.A. Salivonyk, A.G. Sotkis, Y.M. Shuba // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 100-104. — Бібліогр.: 27 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | In recent years gold nanoparticles (AuNPs) have received considerable attention for various biomedical applications including diagnostics and targeted drug delivery. However, more research is still needed to characterize such aspects of their use in clinical oncology as permeability, retention and functional effect on tumor cells. Aims: This study was designed to describe the effect of non-functionalized AuNPs on LNCaP prostate cancer cells growth. Material and Methods: LNCaP cells were cultured in RPMI-1640 medium containing AuNPs covered by polyvinylpyrrolidone of average size 26.4 nm (10.0 μg/ml). Counts of cells were calculated and their morphology was examined. Results: AuNPs conglomerates have been visualized in cultured cells. After 4-day incubation in presence of AuNPs significant retardation of LNCaP cells growth was observed both in 5α-dihydrotestosterone stimulated and non-stimulated cultures. No morphological changes of live LNCaP cells were seen in any experiment. Conclusion: Given absence of morphological changes in live cells and dribble and relatively constant numbers of dead cells, it was concluded that inhibitory effect of AuNPs on LNCaP cells growth was caused by alterations of proliferation. Key Words: prostate cancer, LNCaP, gold nanoparticles, 5α-dihydrotestosterone.
|
| first_indexed | 2025-12-07T18:56:52Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145463 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T18:56:52Z |
| publishDate | 2015 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Reznikov, A.G. Salivonyk, O.A. Sotkis, A.G. Shuba, Y.M. 2019-01-22T11:04:12Z 2019-01-22T11:04:12Z 2015 Assessment of gold nanoparticle effect on prostate cancer LNCaP cells / A.G. Reznikov, O.A. Salivonyk, A.G. Sotkis, Y.M. Shuba // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 100-104. — Бібліогр.: 27 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145463 In recent years gold nanoparticles (AuNPs) have received considerable attention for various biomedical applications including diagnostics and targeted drug delivery. However, more research is still needed to characterize such aspects of their use in clinical oncology as permeability, retention and functional effect on tumor cells. Aims: This study was designed to describe the effect of non-functionalized AuNPs on LNCaP prostate cancer cells growth. Material and Methods: LNCaP cells were cultured in RPMI-1640 medium containing AuNPs covered by polyvinylpyrrolidone of average size 26.4 nm (10.0 μg/ml). Counts of cells were calculated and their morphology was examined. Results: AuNPs conglomerates have been visualized in cultured cells. After 4-day incubation in presence of AuNPs significant retardation of LNCaP cells growth was observed both in 5α-dihydrotestosterone stimulated and non-stimulated cultures. No morphological changes of live LNCaP cells were seen in any experiment. Conclusion: Given absence of morphological changes in live cells and dribble and relatively constant numbers of dead cells, it was concluded that inhibitory effect of AuNPs on LNCaP cells growth was caused by alterations of proliferation. Key Words: prostate cancer, LNCaP, gold nanoparticles, 5α-dihydrotestosterone. The authors are grateful to Dr. A.V. Usatenko, Director of the Research Institute of Nanotechnological Industry, Open International University of Human Development “Ukraine”, for donating AuNPs for the study. We would like to thank Dr. O.Yu. Chunikhin (O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine) for his kind help on carrying out laser spectroscopy of the AuNPs preparation. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Assessment of gold nanoparticle effect on prostate cancer LNCaP cells Article published earlier |
| spellingShingle | Assessment of gold nanoparticle effect on prostate cancer LNCaP cells Reznikov, A.G. Salivonyk, O.A. Sotkis, A.G. Shuba, Y.M. Original contributions |
| title | Assessment of gold nanoparticle effect on prostate cancer LNCaP cells |
| title_full | Assessment of gold nanoparticle effect on prostate cancer LNCaP cells |
| title_fullStr | Assessment of gold nanoparticle effect on prostate cancer LNCaP cells |
| title_full_unstemmed | Assessment of gold nanoparticle effect on prostate cancer LNCaP cells |
| title_short | Assessment of gold nanoparticle effect on prostate cancer LNCaP cells |
| title_sort | assessment of gold nanoparticle effect on prostate cancer lncap cells |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145463 |
| work_keys_str_mv | AT reznikovag assessmentofgoldnanoparticleeffectonprostatecancerlncapcells AT salivonykoa assessmentofgoldnanoparticleeffectonprostatecancerlncapcells AT sotkisag assessmentofgoldnanoparticleeffectonprostatecancerlncapcells AT shubaym assessmentofgoldnanoparticleeffectonprostatecancerlncapcells |