Prognostic significance of MDM2 gene expression in childhood neuroblastoma
Aim: To investigate the association of MDM2 expression at the mRNA levels in neuroblastoma with clinical features and unfavorable disease factors to determine the possibility of it usage as a prognostic marker of neuroblastoma. Materials and Methods: Total RNA and DNA were extracted from tumor tissu...
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| Опубліковано в: : | Experimental Oncology |
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| Дата: | 2015 |
| Автори: | , , , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2015
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| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/145465 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Prognostic significance of MDM2 gene expression in childhood neuroblastoma / M.V. Inomistova, N.M. Svergun, N.M. Khranovska, O.V. Skachkova, O.I. Gorbach, G.I. Klymnyuk // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 111-115. — Бібліогр.: 15 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862744048399286272 |
|---|---|
| author | Inomistova, M.V. Svergun, N.M. Khranovska, N.M. Skachkova, O.V. Gorbach, O.I. Klymnyuk, G.I. |
| author_facet | Inomistova, M.V. Svergun, N.M. Khranovska, N.M. Skachkova, O.V. Gorbach, O.I. Klymnyuk, G.I. |
| citation_txt | Prognostic significance of MDM2 gene expression in childhood neuroblastoma / M.V. Inomistova, N.M. Svergun, N.M. Khranovska, O.V. Skachkova, O.I. Gorbach, G.I. Klymnyuk // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 111-115. — Бібліогр.: 15 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To investigate the association of MDM2 expression at the mRNA levels in neuroblastoma with clinical features and unfavorable disease factors to determine the possibility of it usage as a prognostic marker of neuroblastoma. Materials and Methods: Total RNA and DNA were extracted from tumor tissue samples of total 91 neuroblastoma patients (mean age: 39.45 ± 4.81 months). MDM2 mRNA levels were detected with Q-PCR. TP53 gene deletion was detected with FISH method. MYCN amplification was detected with Q-PCR analysis in fresh tumor samples and FISH in FFPE samples. Results: We investigated the association of MDM2 mRNA expression with clinical outcome in neuroblastoma patients (n = 91). Kaplan — Meier curves showed a significant association of high MDM2 expression with poor event-free survival (p < 0.001). Clinical outcome of patients without MYCN amplification with low MDM2 expression was associated with better event-free survival than with high MDM2 expression (p < 0.001). Overexpression of MDM2 can be used as significant prognostic marker for patient stratification on risk groups and treatment optimization. Conclusion: Our results showed that the high expression of MDM2 at mRNA levels is an important factor of neuroblastoma prognosis. It may be a valuable additional molecular marker in guiding specific therapy in MYCN non-amplified NB patients without TP53 gene deletion. Key Words: neuroblastoma, MDM2 mRNA expression, MYCN gene amplification, p53/MDM2 interaction.
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| first_indexed | 2025-12-07T20:33:15Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145465 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T20:33:15Z |
| publishDate | 2015 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Inomistova, M.V. Svergun, N.M. Khranovska, N.M. Skachkova, O.V. Gorbach, O.I. Klymnyuk, G.I. 2019-01-22T11:05:58Z 2019-01-22T11:05:58Z 2015 Prognostic significance of MDM2 gene expression in childhood neuroblastoma / M.V. Inomistova, N.M. Svergun, N.M. Khranovska, O.V. Skachkova, O.I. Gorbach, G.I. Klymnyuk // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 111-115. — Бібліогр.: 15 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145465 Aim: To investigate the association of MDM2 expression at the mRNA levels in neuroblastoma with clinical features and unfavorable disease factors to determine the possibility of it usage as a prognostic marker of neuroblastoma. Materials and Methods: Total RNA and DNA were extracted from tumor tissue samples of total 91 neuroblastoma patients (mean age: 39.45 ± 4.81 months). MDM2 mRNA levels were detected with Q-PCR. TP53 gene deletion was detected with FISH method. MYCN amplification was detected with Q-PCR analysis in fresh tumor samples and FISH in FFPE samples. Results: We investigated the association of MDM2 mRNA expression with clinical outcome in neuroblastoma patients (n = 91). Kaplan — Meier curves showed a significant association of high MDM2 expression with poor event-free survival (p < 0.001). Clinical outcome of patients without MYCN amplification with low MDM2 expression was associated with better event-free survival than with high MDM2 expression (p < 0.001). Overexpression of MDM2 can be used as significant prognostic marker for patient stratification on risk groups and treatment optimization. Conclusion: Our results showed that the high expression of MDM2 at mRNA levels is an important factor of neuroblastoma prognosis. It may be a valuable additional molecular marker in guiding specific therapy in MYCN non-amplified NB patients without TP53 gene deletion. Key Words: neuroblastoma, MDM2 mRNA expression, MYCN gene amplification, p53/MDM2 interaction. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Prognostic significance of MDM2 gene expression in childhood neuroblastoma Article published earlier |
| spellingShingle | Prognostic significance of MDM2 gene expression in childhood neuroblastoma Inomistova, M.V. Svergun, N.M. Khranovska, N.M. Skachkova, O.V. Gorbach, O.I. Klymnyuk, G.I. Original contributions |
| title | Prognostic significance of MDM2 gene expression in childhood neuroblastoma |
| title_full | Prognostic significance of MDM2 gene expression in childhood neuroblastoma |
| title_fullStr | Prognostic significance of MDM2 gene expression in childhood neuroblastoma |
| title_full_unstemmed | Prognostic significance of MDM2 gene expression in childhood neuroblastoma |
| title_short | Prognostic significance of MDM2 gene expression in childhood neuroblastoma |
| title_sort | prognostic significance of mdm2 gene expression in childhood neuroblastoma |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145465 |
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