Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vul...
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| Опубліковано в: : | Experimental Oncology |
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| Дата: | 2015 |
| Автори: | , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2015
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| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/145466 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862746285924155392 |
|---|---|
| author | Kochubei, T.O. Maksymchuk, O.V. Piven, O.O. Lukash, L.L. |
| author_facet | Kochubei, T.O. Maksymchuk, O.V. Piven, O.O. Lukash, L.L. |
| citation_txt | Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. Results: PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3. Key Words: phytohemagglutinin, isolectins, apoptosis, caspase-3, Bax.
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| first_indexed | 2025-12-07T20:45:25Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145466 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T20:45:25Z |
| publishDate | 2015 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Kochubei, T.O. Maksymchuk, O.V. Piven, O.O. Lukash, L.L. 2019-01-22T11:32:48Z 2019-01-22T11:32:48Z 2015 Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145466 Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. Results: PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3. Key Words: phytohemagglutinin, isolectins, apoptosis, caspase-3, Bax. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro Article published earlier |
| spellingShingle | Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro Kochubei, T.O. Maksymchuk, O.V. Piven, O.O. Lukash, L.L. Original contributions |
| title | Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_full | Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_fullStr | Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_full_unstemmed | Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_short | Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_sort | isolectins of phytohemagglutinin are able to induce apoptosis in hep-2 carcinoma cells in vitro |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145466 |
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