Pharmacological effect of aminoferrocene in mice with L1210 leukemia
Aim: To study the cytostatic and some biological effects of aminoferrocene using mice with L1210 lymphoid leukemia. Materials and Methods: Experiments were performed on BDF1 male mice (DBA/2, female × C57Bl/6, male) with transplantable L1210 lymphoid leukemia. Determination of antitumor activity of...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2015 |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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| Zitieren: | Pharmacological effect of aminoferrocene in mice with L1210 leukemia / V.F. Chekhun, A. Mokhir, S. Daum, I.N. Todor, N.Yu. Lukianova, Yu.V. Shvets, A.P. Burlaka // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 120-125. — Бібліогр.: 34 назв. — англ. |
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Chekhun, V.F. Mokhir, A. Daum, S. Todor, I.N. Lukianova, N.Yu. Shvets, Yu.V. Burlaka, A.P. 2019-01-22T11:33:40Z 2019-01-22T11:33:40Z 2015 Pharmacological effect of aminoferrocene in mice with L1210 leukemia / V.F. Chekhun, A. Mokhir, S. Daum, I.N. Todor, N.Yu. Lukianova, Yu.V. Shvets, A.P. Burlaka // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 120-125. — Бібліогр.: 34 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145467 Aim: To study the cytostatic and some biological effects of aminoferrocene using mice with L1210 lymphoid leukemia. Materials and Methods: Experiments were performed on BDF1 male mice (DBA/2, female × C57Bl/6, male) with transplantable L1210 lymphoid leukemia. Determination of antitumor activity of Benzyl-Fc Boron (Bn), it was injected intraperitoneally 6 times daily, starting on day 2 after L1210 leukemia cell transplantation. Doses of Bn such as 26; 260 and 2600 μg/kg were used. The determination of intracellular content of cardiolipin, thiols, reactive oxygen species (ROS) and also analysis of Annexin V positivity and mitochondrial transmembrane potential (JC-1 staining) were performed with use of flow cytometry. The levels of “free iron” complexes, transferrin active forms and the rate of NO generation were measured by EPR-specroscopy. Results: Six daily injections of Bn at a dose of 26 μg/kg resulted in an increased survival of mice with L1210 leukemia by 28% (p < 0.05). Bn led to an increase of apoptotic cells number and ROS amount in leukemia cells. Besides, Bn caused a decrease of cardiolipin and nonprotein thiol compounds content. The membrane electrochemical potential of cell mitochondria was decreased also after Bn administration. Studies using EPR-spectroscopy revealed a significant increase in a level of “free iron”, content of transferrin active species and generation rate of NO by inducible NO-synthase in L1210 cells after aminoferrocene administration. Conclusion: Our data indicate that Benzyl-Fc Boron can be promising candidate for realizing a new strategy of anticancer therapy with the use of ROS-inducing agents. Key Words: aminoferrocene, tumor, L1210 leukemia, mitochondria membrane electrochemical potential, cardiolipin, thiols, ROS, transferrin, NO-generation. This work was supported by: 1. German Research Council (DFG), project № MO 1418/7–1 “Aminoferrocene dendrimers as prodrugs for the therapy of hematologic cancers derived from B-cells”. 2. State Aim Scientific-Technical Programme of Ukraine “Nanotechnology and nanomaterials” (Project № 5.18.3.53 “Biological activity and safety of nanomaterials which can be use for development of antitumor drugs vector systems”). 3. Aim Complex Programme of Fundamental Investigations of NAS of Ukraine (Project № 96/14-H “Interaction of nanostructural materials with normal and tumor cells, development of delivery methods, safety of their use”). en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Pharmacological effect of aminoferrocene in mice with L1210 leukemia Article published earlier |
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| title |
Pharmacological effect of aminoferrocene in mice with L1210 leukemia |
| spellingShingle |
Pharmacological effect of aminoferrocene in mice with L1210 leukemia Chekhun, V.F. Mokhir, A. Daum, S. Todor, I.N. Lukianova, N.Yu. Shvets, Yu.V. Burlaka, A.P. Original contributions |
| title_short |
Pharmacological effect of aminoferrocene in mice with L1210 leukemia |
| title_full |
Pharmacological effect of aminoferrocene in mice with L1210 leukemia |
| title_fullStr |
Pharmacological effect of aminoferrocene in mice with L1210 leukemia |
| title_full_unstemmed |
Pharmacological effect of aminoferrocene in mice with L1210 leukemia |
| title_sort |
pharmacological effect of aminoferrocene in mice with l1210 leukemia |
| author |
Chekhun, V.F. Mokhir, A. Daum, S. Todor, I.N. Lukianova, N.Yu. Shvets, Yu.V. Burlaka, A.P. |
| author_facet |
Chekhun, V.F. Mokhir, A. Daum, S. Todor, I.N. Lukianova, N.Yu. Shvets, Yu.V. Burlaka, A.P. |
| topic |
Original contributions |
| topic_facet |
Original contributions |
| publishDate |
2015 |
| language |
English |
| container_title |
Experimental Oncology |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| format |
Article |
| description |
Aim: To study the cytostatic and some biological effects of aminoferrocene using mice with L1210 lymphoid leukemia. Materials and Methods: Experiments were performed on BDF1 male mice (DBA/2, female × C57Bl/6, male) with transplantable L1210 lymphoid leukemia. Determination of antitumor activity of Benzyl-Fc Boron (Bn), it was injected intraperitoneally 6 times daily, starting on day 2 after L1210 leukemia cell transplantation. Doses of Bn such as 26; 260 and 2600 μg/kg were used. The determination of intracellular content of cardiolipin, thiols, reactive oxygen species (ROS) and also analysis of Annexin V positivity and mitochondrial transmembrane potential (JC-1 staining) were performed with use of flow cytometry. The levels of “free iron” complexes, transferrin active forms and the rate of NO generation were measured by EPR-specroscopy. Results: Six daily injections of Bn at a dose of 26 μg/kg resulted in an increased survival of mice with L1210 leukemia by 28% (p < 0.05). Bn led to an increase of apoptotic cells number and ROS amount in leukemia cells. Besides, Bn caused a decrease of cardiolipin and nonprotein thiol compounds content. The membrane electrochemical potential of cell mitochondria was decreased also after Bn administration. Studies using EPR-spectroscopy revealed a significant increase in a level of “free iron”, content of transferrin active species and generation rate of NO by inducible NO-synthase in L1210 cells after aminoferrocene administration. Conclusion: Our data indicate that Benzyl-Fc Boron can be promising candidate for realizing a new strategy of anticancer therapy with the use of ROS-inducing agents. Key Words: aminoferrocene, tumor, L1210 leukemia, mitochondria membrane electrochemical potential, cardiolipin, thiols, ROS, transferrin, NO-generation.
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| issn |
1812-9269 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/145467 |
| citation_txt |
Pharmacological effect of aminoferrocene in mice with L1210 leukemia / V.F. Chekhun, A. Mokhir, S. Daum, I.N. Todor, N.Yu. Lukianova, Yu.V. Shvets, A.P. Burlaka // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 120-125. — Бібліогр.: 34 назв. — англ. |
| work_keys_str_mv |
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| first_indexed |
2025-12-01T01:56:03Z |
| last_indexed |
2025-12-01T01:56:03Z |
| _version_ |
1850859042496315392 |