Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16

Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16

Aim: To study the effects of xenogeneic cancer vaccine (XCV) developed on the basis of nervous tissue antigen from rat embryo of late gestation period and protein-containing metabolite of Bacillus subtilis with molecular weight of 70 kDa, on specific and unspecific antitumor reactions of cellular an...

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Published in:Experimental Oncology
Date:2015
Main Authors: Fedosova, N.I., Voeykova, I.M., Karaman, O.M., Symchych, T.V., Didenko, G.V., Lisovenko, G.S., Evstratieva, L.M., Potebnya, G.P.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/145475
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16 / N.I. Fedosova, I.M. Voeykova, O.M. Karaman, T.V. Symchych, G.V. Didenko, G.S. Lisovenko, L.M. Evstratieva, G.P. Potebnya // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 130-134. — Бібліогр.: 38 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145475
record_format dspace
spelling Fedosova, N.I.
Voeykova, I.M.
Karaman, O.M.
Symchych, T.V.
Didenko, G.V.
Lisovenko, G.S.
Evstratieva, L.M.
Potebnya, G.P.
2019-01-22T11:46:42Z
2019-01-22T11:46:42Z
2015
Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16 / N.I. Fedosova, I.M. Voeykova, O.M. Karaman, T.V. Symchych, G.V. Didenko, G.S. Lisovenko, L.M. Evstratieva, G.P. Potebnya // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 130-134. — Бібліогр.: 38 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145475
Aim: To study the effects of xenogeneic cancer vaccine (XCV) developed on the basis of nervous tissue antigen from rat embryo of late gestation period and protein-containing metabolite of Bacillus subtilis with molecular weight of 70 kDa, on specific and unspecific antitumor reactions of cellular and humoral chains of immune system, and to analyze possible mechanisms of its antimetastatic action. Materials and Methods: XCV was administered triply with 3-day intervals after surgical removal of experimental melanoma В-16 in C57Bl/6 mice. Cytotoxic activity (CTA) of splenocytes against target cells К-562 as well as CTA of splenocytes, peritoneal macrophages (PM) and blood serum against melanoma В-16 target cells were determined using МТТ test. The content of circulating immune complexes (CIC) in blood serum was evaluated by precipitation reaction. Results: Immunologic effects of XCV vaccination in experimental animals with surgically removed melanoma B-16 in comparison with similarly treated unvaccinated mice were as follows: prevention of medium molecular weight CIC accumulation in blood serum during all observation period, significant increase (р < 0.05) of CTA of effectors of unspecific antitumor immunity (natural killer cells — NK — by 25.5 ± 1.7 vs 12.5 ± 5.4%, and PM — by 37.3 ± 0.6 vs 32.0 ± 0.9%, respectively) at 37th day after the surgery, and also preservation of functional activity of specific cytotoxic lymphocytes at the level of intact control. Conclusion: The results of the study allow propose that antimetastatic effect of XCV vaccination could be based on increased CTA of NK and PM, and preservation of CTL functional activity at late terms after surgical removal of B-16 primary tumors. Key Words: xenogeneic cancer vaccine, melanoma В-16, natural killer cells, macrophages, cytotoxic lymphocytes, cytotoxic activity, antimetastatic activity.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
spellingShingle Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
Fedosova, N.I.
Voeykova, I.M.
Karaman, O.M.
Symchych, T.V.
Didenko, G.V.
Lisovenko, G.S.
Evstratieva, L.M.
Potebnya, G.P.
Original contributions
title_short Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
title_full Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
title_fullStr Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
title_full_unstemmed Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
title_sort cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma b-16
author Fedosova, N.I.
Voeykova, I.M.
Karaman, O.M.
Symchych, T.V.
Didenko, G.V.
Lisovenko, G.S.
Evstratieva, L.M.
Potebnya, G.P.
author_facet Fedosova, N.I.
Voeykova, I.M.
Karaman, O.M.
Symchych, T.V.
Didenko, G.V.
Lisovenko, G.S.
Evstratieva, L.M.
Potebnya, G.P.
topic Original contributions
topic_facet Original contributions
publishDate 2015
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To study the effects of xenogeneic cancer vaccine (XCV) developed on the basis of nervous tissue antigen from rat embryo of late gestation period and protein-containing metabolite of Bacillus subtilis with molecular weight of 70 kDa, on specific and unspecific antitumor reactions of cellular and humoral chains of immune system, and to analyze possible mechanisms of its antimetastatic action. Materials and Methods: XCV was administered triply with 3-day intervals after surgical removal of experimental melanoma В-16 in C57Bl/6 mice. Cytotoxic activity (CTA) of splenocytes against target cells К-562 as well as CTA of splenocytes, peritoneal macrophages (PM) and blood serum against melanoma В-16 target cells were determined using МТТ test. The content of circulating immune complexes (CIC) in blood serum was evaluated by precipitation reaction. Results: Immunologic effects of XCV vaccination in experimental animals with surgically removed melanoma B-16 in comparison with similarly treated unvaccinated mice were as follows: prevention of medium molecular weight CIC accumulation in blood serum during all observation period, significant increase (р < 0.05) of CTA of effectors of unspecific antitumor immunity (natural killer cells — NK — by 25.5 ± 1.7 vs 12.5 ± 5.4%, and PM — by 37.3 ± 0.6 vs 32.0 ± 0.9%, respectively) at 37th day after the surgery, and also preservation of functional activity of specific cytotoxic lymphocytes at the level of intact control. Conclusion: The results of the study allow propose that antimetastatic effect of XCV vaccination could be based on increased CTA of NK and PM, and preservation of CTL functional activity at late terms after surgical removal of B-16 primary tumors. Key Words: xenogeneic cancer vaccine, melanoma В-16, natural killer cells, macrophages, cytotoxic lymphocytes, cytotoxic activity, antimetastatic activity.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145475
citation_txt Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16 / N.I. Fedosova, I.M. Voeykova, O.M. Karaman, T.V. Symchych, G.V. Didenko, G.S. Lisovenko, L.M. Evstratieva, G.P. Potebnya // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 130-134. — Бібліогр.: 38 назв. — англ.
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AT karamanom cytotoxicactivityofimmunecellsfollowingadministrationofxenogeneiccancervaccineinmicewithmelanomab16
AT symchychtv cytotoxicactivityofimmunecellsfollowingadministrationofxenogeneiccancervaccineinmicewithmelanomab16
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first_indexed 2025-12-07T17:54:20Z
last_indexed 2025-12-07T17:54:20Z
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