Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells
Aim: To assess the role of endogenous lactoferrin (LF) in the formation of the molecular phenotype of human breast cancer (BC) cell lines with varying degrees of malignancy, including cisplatin/doxorubicin resistant cell lines, and identify possible impact of exogenous LF. Materials and Methods: 5 b...
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| Опубліковано в: : | Experimental Oncology |
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| Дата: | 2015 |
| Автори: | , , , , , , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2015
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| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/145485 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells / V.F. Chekhun, I.V. Zalutskii, L.A. Naleskina, N.Yu. Lukianova, T.M. Yalovenko, T.V. Borikun, S.O. Sobchenko, I.V. Semak, V.S. Lukashevich // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 181-186. — Бібліогр.: 33 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862694641169596416 |
|---|---|
| author | Chekhun, V.F. Zalutskii, I.V. Naleskina, L.A. Lukianova, N.Yu. Yalovenko, T.M. Borikun, T.V. Sobchenko, S.O. Semak, I.V. Lukashevich, V.S. |
| author_facet | Chekhun, V.F. Zalutskii, I.V. Naleskina, L.A. Lukianova, N.Yu. Yalovenko, T.M. Borikun, T.V. Sobchenko, S.O. Semak, I.V. Lukashevich, V.S. |
| citation_txt | Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells / V.F. Chekhun, I.V. Zalutskii, L.A. Naleskina, N.Yu. Lukianova, T.M. Yalovenko, T.V. Borikun, S.O. Sobchenko, I.V. Semak, V.S. Lukashevich // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 181-186. — Бібліогр.: 33 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To assess the role of endogenous lactoferrin (LF) in the formation of the molecular phenotype of human breast cancer (BC) cell lines with varying degrees of malignancy, including cisplatin/doxorubicin resistant cell lines, and identify possible impact of exogenous LF. Materials and Methods: 5 breast cell lines of different origin — MCF-10 A, MCF-7, including doxorubicin/cisplatin resistant ones, T47D, MDA-MB-231, and MDA-MB-468. Immunocytochemistry: expression of LF, Ki-67, adhesion molecules E- and N-cadherin, CD44, CD24 rating the invasive potential of cells. Results: Expression of LF in human BC cell lines varies. It is associated with the heterogeneity of molecular profiles of cell lines in terms of adhesion. A link has been established between the level of LF expression in the resistant cell line MCF-7/CP and MCF-7/Dox, features of their molecular profile and invasive properties. Exogenous LF was shown to be capable of modifying the molecular profile and invasive properties of all the studied cell lines including resistant ones (MCF-7/CP and MCF-7/Dox). Conclusions: The sensitivity of cytostatic-resistant cell lines (MCF-7/CP and MCF-7/Dox) tends to increase under the influence of exogenous LF. It is likely that this effect is due to LF-mediated inhibition of the expression of proteins associated with drug resistance. Key Words: lactoferrin, cell lines of human breast cancer, molecular phenotype, proliferative activity, invasive potential, adhesion molecules.
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| first_indexed | 2025-12-07T16:23:15Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145485 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T16:23:15Z |
| publishDate | 2015 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Chekhun, V.F. Zalutskii, I.V. Naleskina, L.A. Lukianova, N.Yu. Yalovenko, T.M. Borikun, T.V. Sobchenko, S.O. Semak, I.V. Lukashevich, V.S. 2019-01-22T12:48:41Z 2019-01-22T12:48:41Z 2015 Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells / V.F. Chekhun, I.V. Zalutskii, L.A. Naleskina, N.Yu. Lukianova, T.M. Yalovenko, T.V. Borikun, S.O. Sobchenko, I.V. Semak, V.S. Lukashevich // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 181-186. — Бібліогр.: 33 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145485 Aim: To assess the role of endogenous lactoferrin (LF) in the formation of the molecular phenotype of human breast cancer (BC) cell lines with varying degrees of malignancy, including cisplatin/doxorubicin resistant cell lines, and identify possible impact of exogenous LF. Materials and Methods: 5 breast cell lines of different origin — MCF-10 A, MCF-7, including doxorubicin/cisplatin resistant ones, T47D, MDA-MB-231, and MDA-MB-468. Immunocytochemistry: expression of LF, Ki-67, adhesion molecules E- and N-cadherin, CD44, CD24 rating the invasive potential of cells. Results: Expression of LF in human BC cell lines varies. It is associated with the heterogeneity of molecular profiles of cell lines in terms of adhesion. A link has been established between the level of LF expression in the resistant cell line MCF-7/CP and MCF-7/Dox, features of their molecular profile and invasive properties. Exogenous LF was shown to be capable of modifying the molecular profile and invasive properties of all the studied cell lines including resistant ones (MCF-7/CP and MCF-7/Dox). Conclusions: The sensitivity of cytostatic-resistant cell lines (MCF-7/CP and MCF-7/Dox) tends to increase under the influence of exogenous LF. It is likely that this effect is due to LF-mediated inhibition of the expression of proteins associated with drug resistance. Key Words: lactoferrin, cell lines of human breast cancer, molecular phenotype, proliferative activity, invasive potential, adhesion molecules. The study was supported by joint scientific project of the NAS of Ukraine and the NAS of Belarus 2015–2016 “Molecular-Biological Effects and Mechanisms of Lactoferrin Action on Tumor Cells in vitro and in vivo”. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells Article published earlier |
| spellingShingle | Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells Chekhun, V.F. Zalutskii, I.V. Naleskina, L.A. Lukianova, N.Yu. Yalovenko, T.M. Borikun, T.V. Sobchenko, S.O. Semak, I.V. Lukashevich, V.S. Original contributions |
| title | Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells |
| title_full | Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells |
| title_fullStr | Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells |
| title_full_unstemmed | Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells |
| title_short | Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells |
| title_sort | modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145485 |
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