Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells

Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells

Aim: To assess the role of endogenous lactoferrin (LF) in the formation of the molecular phenotype of human breast cancer (BC) cell lines with varying degrees of malignancy, including cisplatin/doxorubicin resistant cell lines, and identify possible impact of exogenous LF. Materials and Methods: 5 b...

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Veröffentlicht in:Experimental Oncology
Datum:2015
Hauptverfasser: Chekhun, V.F., Zalutskii, I.V., Naleskina, L.A., Lukianova, N.Yu., Yalovenko, T.M., Borikun, T.V., Sobchenko, S.O., Semak, I.V., Lukashevich, V.S.
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Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/145485
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Zitieren:Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells / V.F. Chekhun, I.V. Zalutskii, L.A. Naleskina, N.Yu. Lukianova, T.M. Yalovenko, T.V. Borikun, S.O. Sobchenko, I.V. Semak, V.S. Lukashevich // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 181-186. — Бібліогр.: 33 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145485
record_format dspace
spelling Chekhun, V.F.
Zalutskii, I.V.
Naleskina, L.A.
Lukianova, N.Yu.
Yalovenko, T.M.
Borikun, T.V.
Sobchenko, S.O.
Semak, I.V.
Lukashevich, V.S.
2019-01-22T12:48:41Z
2019-01-22T12:48:41Z
2015
Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells / V.F. Chekhun, I.V. Zalutskii, L.A. Naleskina, N.Yu. Lukianova, T.M. Yalovenko, T.V. Borikun, S.O. Sobchenko, I.V. Semak, V.S. Lukashevich // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 181-186. — Бібліогр.: 33 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145485
Aim: To assess the role of endogenous lactoferrin (LF) in the formation of the molecular phenotype of human breast cancer (BC) cell lines with varying degrees of malignancy, including cisplatin/doxorubicin resistant cell lines, and identify possible impact of exogenous LF. Materials and Methods: 5 breast cell lines of different origin — MCF-10 A, MCF-7, including doxorubicin/cisplatin resistant ones, T47D, MDA-MB-231, and MDA-MB-468. Immunocytochemistry: expression of LF, Ki-67, adhesion molecules E- and N-cadherin, CD44, CD24 rating the invasive potential of cells. Results: Expression of LF in human BC cell lines varies. It is associated with the heterogeneity of molecular profiles of cell lines in terms of adhesion. A link has been established between the level of LF expression in the resistant cell line MCF-7/CP and MCF-7/Dox, features of their molecular profile and invasive properties. Exogenous LF was shown to be capable of modifying the molecular profile and invasive properties of all the studied cell lines including resistant ones (MCF-7/CP and MCF-7/Dox). Conclusions: The sensitivity of cytostatic-resistant cell lines (MCF-7/CP and MCF-7/Dox) tends to increase under the influence of exogenous LF. It is likely that this effect is due to LF-mediated inhibition of the expression of proteins associated with drug resistance. Key Words: lactoferrin, cell lines of human breast cancer, molecular phenotype, proliferative activity, invasive potential, adhesion molecules.
The study was supported by joint scientific pro­ject of the NAS of Ukraine and the NAS of Belarus 2015–2016 “Molecular-Biological Effects and Mechanisms of Lactoferrin Action on Tumor Cells in vitro and in vivo”.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells
spellingShingle Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells
Chekhun, V.F.
Zalutskii, I.V.
Naleskina, L.A.
Lukianova, N.Yu.
Yalovenko, T.M.
Borikun, T.V.
Sobchenko, S.O.
Semak, I.V.
Lukashevich, V.S.
Original contributions
title_short Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells
title_full Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells
title_fullStr Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells
title_full_unstemmed Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells
title_sort modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells
author Chekhun, V.F.
Zalutskii, I.V.
Naleskina, L.A.
Lukianova, N.Yu.
Yalovenko, T.M.
Borikun, T.V.
Sobchenko, S.O.
Semak, I.V.
Lukashevich, V.S.
author_facet Chekhun, V.F.
Zalutskii, I.V.
Naleskina, L.A.
Lukianova, N.Yu.
Yalovenko, T.M.
Borikun, T.V.
Sobchenko, S.O.
Semak, I.V.
Lukashevich, V.S.
topic Original contributions
topic_facet Original contributions
publishDate 2015
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To assess the role of endogenous lactoferrin (LF) in the formation of the molecular phenotype of human breast cancer (BC) cell lines with varying degrees of malignancy, including cisplatin/doxorubicin resistant cell lines, and identify possible impact of exogenous LF. Materials and Methods: 5 breast cell lines of different origin — MCF-10 A, MCF-7, including doxorubicin/cisplatin resistant ones, T47D, MDA-MB-231, and MDA-MB-468. Immunocytochemistry: expression of LF, Ki-67, adhesion molecules E- and N-cadherin, CD44, CD24 rating the invasive potential of cells. Results: Expression of LF in human BC cell lines varies. It is associated with the heterogeneity of molecular profiles of cell lines in terms of adhesion. A link has been established between the level of LF expression in the resistant cell line MCF-7/CP and MCF-7/Dox, features of their molecular profile and invasive properties. Exogenous LF was shown to be capable of modifying the molecular profile and invasive properties of all the studied cell lines including resistant ones (MCF-7/CP and MCF-7/Dox). Conclusions: The sensitivity of cytostatic-resistant cell lines (MCF-7/CP and MCF-7/Dox) tends to increase under the influence of exogenous LF. It is likely that this effect is due to LF-mediated inhibition of the expression of proteins associated with drug resistance. Key Words: lactoferrin, cell lines of human breast cancer, molecular phenotype, proliferative activity, invasive potential, adhesion molecules.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145485
citation_txt Modifying effects of lactoferrin in vitro on molecular phenotype of human breast cancer cells / V.F. Chekhun, I.V. Zalutskii, L.A. Naleskina, N.Yu. Lukianova, T.M. Yalovenko, T.V. Borikun, S.O. Sobchenko, I.V. Semak, V.S. Lukashevich // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 181-186. — Бібліогр.: 33 назв. — англ.
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first_indexed 2025-12-07T16:23:15Z
last_indexed 2025-12-07T16:23:15Z
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