Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells

Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells

Aim: To evaluate the viability of leukemic cells sensitive (L1210S) and resistant (L1210R) to cisplatin, ROS production and free cytosolic Ca²⁺ concentration under treatment with cisplatin or its combination with photoexcited fullerene C₆₀. Methods: Cell viability was assessed by the MTT reduction a...

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Бібліографічні деталі
Опубліковано в: :Experimental Oncology
Дата:2015
Автори: Franskevych, D.V., Prylutska, S.V., Grynyuk, I.I., Grebinyk, D.M., Matyshevska, O.P.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
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Original contributions
Онлайн доступ:https://nasplib.isofts.kiev.ua/handle/123456789/145486
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells / D.V. Franskevych, S.V. Prylutska, I.I. Grynyuk, D.M. Grebinyk, O.P. Matyshevska // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 187-191. — Бібліогр.: 34 назв. — англ.

Репозитарії

Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145486
record_format dspace
spelling Franskevych, D.V.
Prylutska, S.V.
Grynyuk, I.I.
Grebinyk, D.M.
Matyshevska, O.P.
2019-01-22T12:56:03Z
2019-01-22T12:56:03Z
2015
Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells / D.V. Franskevych, S.V. Prylutska, I.I. Grynyuk, D.M. Grebinyk, O.P. Matyshevska // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 187-191. — Бібліогр.: 34 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145486
Aim: To evaluate the viability of leukemic cells sensitive (L1210S) and resistant (L1210R) to cisplatin, ROS production and free cytosolic Ca²⁺ concentration under treatment with cisplatin or its combination with photoexcited fullerene C₆₀. Methods: Cell viability was assessed by the MTT reduction assay. Light-emitting diode lamp (2.45 J/сm2) was used for photoexcitation of intracellular accumulated fullerene C₆₀. Free cytosolic calcium concentration ([Ca²⁺]i) and ROS production in cells were estimated with the use of fluorescent probes Indo-1 and 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA), respectively. Results: It is shown that viability of L1210R cells wasn’t changed under treatment with cisplatin in concentration range 0.1–10 μg/ml. 50% and 30% decrease of L1210S cells were observed after 24 h of incubation with cisplatin at concentrations 5 and 1 μg/ml, respectively. Intensification of extranuclear cytotoxic effects (ROS production and [Ca²⁺]iincrease) after treatment with 1 μg/ml was detected in L1210S, but not in L1210R cells. The most strongly pronounced increase of ROS production and [Ca²⁺]i in both L1210 cell lines was revealed in dynamics after combined treatment with cisplatin (1 μg/ml) and photoexcited fullerene C₆₀ (10⁻⁵ M) and was followed by decreased viability of not only L1210S, but of L1210R cells as well.. Conclusion: Combined treatment with photoexcited C₆₀ and cisplatin allowed to decrease effective concentration of cisplatin against parental L1210 cells and to increase sensibility of resistant cells to the drug. Key Words: cisplatin, L1210 cells, photoexcited fullerene C₆₀, ROS, cytosolic Ca²⁺, cell viability.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
spellingShingle Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
Franskevych, D.V.
Prylutska, S.V.
Grynyuk, I.I.
Grebinyk, D.M.
Matyshevska, O.P.
Original contributions
title_short Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
title_full Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
title_fullStr Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
title_full_unstemmed Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells
title_sort enhanced cytotoxicity of рhotoexcited fullerene c60 and cisplatin combination against drug-resistant leukemic cells
author Franskevych, D.V.
Prylutska, S.V.
Grynyuk, I.I.
Grebinyk, D.M.
Matyshevska, O.P.
author_facet Franskevych, D.V.
Prylutska, S.V.
Grynyuk, I.I.
Grebinyk, D.M.
Matyshevska, O.P.
topic Original contributions
topic_facet Original contributions
publishDate 2015
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To evaluate the viability of leukemic cells sensitive (L1210S) and resistant (L1210R) to cisplatin, ROS production and free cytosolic Ca²⁺ concentration under treatment with cisplatin or its combination with photoexcited fullerene C₆₀. Methods: Cell viability was assessed by the MTT reduction assay. Light-emitting diode lamp (2.45 J/сm2) was used for photoexcitation of intracellular accumulated fullerene C₆₀. Free cytosolic calcium concentration ([Ca²⁺]i) and ROS production in cells were estimated with the use of fluorescent probes Indo-1 and 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA), respectively. Results: It is shown that viability of L1210R cells wasn’t changed under treatment with cisplatin in concentration range 0.1–10 μg/ml. 50% and 30% decrease of L1210S cells were observed after 24 h of incubation with cisplatin at concentrations 5 and 1 μg/ml, respectively. Intensification of extranuclear cytotoxic effects (ROS production and [Ca²⁺]iincrease) after treatment with 1 μg/ml was detected in L1210S, but not in L1210R cells. The most strongly pronounced increase of ROS production and [Ca²⁺]i in both L1210 cell lines was revealed in dynamics after combined treatment with cisplatin (1 μg/ml) and photoexcited fullerene C₆₀ (10⁻⁵ M) and was followed by decreased viability of not only L1210S, but of L1210R cells as well.. Conclusion: Combined treatment with photoexcited C₆₀ and cisplatin allowed to decrease effective concentration of cisplatin against parental L1210 cells and to increase sensibility of resistant cells to the drug. Key Words: cisplatin, L1210 cells, photoexcited fullerene C₆₀, ROS, cytosolic Ca²⁺, cell viability.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145486
citation_txt Enhanced cytotoxicity of рhotoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells / D.V. Franskevych, S.V. Prylutska, I.I. Grynyuk, D.M. Grebinyk, O.P. Matyshevska // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 187-191. — Бібліогр.: 34 назв. — англ.
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AT prylutskasv enhancedcytotoxicityofrhotoexcitedfullerenec60andcisplatincombinationagainstdrugresistantleukemiccells
AT grynyukii enhancedcytotoxicityofrhotoexcitedfullerenec60andcisplatincombinationagainstdrugresistantleukemiccells
AT grebinykdm enhancedcytotoxicityofrhotoexcitedfullerenec60andcisplatincombinationagainstdrugresistantleukemiccells
AT matyshevskaop enhancedcytotoxicityofrhotoexcitedfullerenec60andcisplatincombinationagainstdrugresistantleukemiccells
first_indexed 2025-12-07T21:15:50Z
last_indexed 2025-12-07T21:15:50Z
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