Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors

Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors

Aim: To synthesize and to study for photodynamic activity a composite photosensitizer consisting of chlorin e6 and human serum albumin nanoparticles (HSA NPs). Materials and Methods: Starting from sorption-purified HSA, the albumin nanoparticles with a different degree of lysine residues cross-linki...

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Published in:Experimental Oncology
Date:2015
Main Authors: Shton, I.O., Sarnatskaya, V.V., Prokopenko, I.V., Gamaleia, N.F.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/145542
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors / I.O. Shton, V.V. Sarnatskaya, I.V. Prokopenko, N.F. Gamaleia // Experimental Oncology. — 2015. — Т. 37, № 4. — С. 250-254. — Бібліогр.: 24 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145542
record_format dspace
spelling Shton, I.O.
Sarnatskaya, V.V.
Prokopenko, I.V.
Gamaleia, N.F.
2019-01-22T20:08:07Z
2019-01-22T20:08:07Z
2015
Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors / I.O. Shton, V.V. Sarnatskaya, I.V. Prokopenko, N.F. Gamaleia // Experimental Oncology. — 2015. — Т. 37, № 4. — С. 250-254. — Бібліогр.: 24 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145542
Aim: To synthesize and to study for photodynamic activity a composite photosensitizer consisting of chlorin e6 and human serum albumin nanoparticles (HSA NPs). Materials and Methods: Starting from sorption-purified HSA, the albumin nanoparticles with a different degree of lysine residues cross-linking (10; 20; 40, and 100%) were obtained by the coacervation method. The HSA NPs were used for synthesis of nanocomposites with chlorin e6 and fluorescein isothiocyanate (FITC)-labled preparations. Malignant lymphocytes of the MT-4 (human T-cell leukemia) line and normal lymphocytes of healthy donors served as cell targets. For photodynamic treatment, a semiconductor laser was exploited as a light source, and cell viability was assessed by MTT or trypan blue dye exclusion tests. For cell imaging and HSA NPs visualization, the fluorescence microscopy and transmission electron microscopy were applied, respectively. C57Bl/6 mice were used in animal experiments. Results: The absorption and fluorescence spectra of chlorin e6-HSA NPs composites were characterized, and by the electron microscopy investigation the size of NPs (nanospheres) was estimated: 100–120 nm. FITC-labled albumin preparations allowed to establish that HSA NPs have much higher exposition and concentration dependent affinity to malignant cell surface than initial HSA. In experiments with MT-4 cells on PDT activity of chlorin e6-HSA NPs, the nanocomposite effectiveness elevated along with increasing percentage of cross-linked aminoacid residues, and for the nanocomposite with 100% of albumin cross-linking it exceeded the activity of free chlorin e6. In contrast to malignant cells, the complexation of chlorin e6 with HSA NPs decreased its photodynamic effect on normal human lymphocytes. Intravenous introduction of the chlorin e6-HSA NPs composite to mice showed prolonged circulation of the nanocomposite in blood in comparison with free PS. Conclusion: Promising results obtained with chlorin e6-HSA NPs composites warrant conduction of full-fledged PDT studies in vivo using the nanocomposites as photosensitizers. Key Words: human serum albumin, nanoparticles, photodynamic therapy, chlorin e6, cell line MT-4, C57Bl/6 mice.
The work of N.F. Gamaleia and I.O. Shton was supported in part by the NATO SPS Programme Multi-Year Project No. 984702.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors
spellingShingle Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors
Shton, I.O.
Sarnatskaya, V.V.
Prokopenko, I.V.
Gamaleia, N.F.
Original contributions
title_short Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors
title_full Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors
title_fullStr Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors
title_full_unstemmed Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors
title_sort chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors
author Shton, I.O.
Sarnatskaya, V.V.
Prokopenko, I.V.
Gamaleia, N.F.
author_facet Shton, I.O.
Sarnatskaya, V.V.
Prokopenko, I.V.
Gamaleia, N.F.
topic Original contributions
topic_facet Original contributions
publishDate 2015
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To synthesize and to study for photodynamic activity a composite photosensitizer consisting of chlorin e6 and human serum albumin nanoparticles (HSA NPs). Materials and Methods: Starting from sorption-purified HSA, the albumin nanoparticles with a different degree of lysine residues cross-linking (10; 20; 40, and 100%) were obtained by the coacervation method. The HSA NPs were used for synthesis of nanocomposites with chlorin e6 and fluorescein isothiocyanate (FITC)-labled preparations. Malignant lymphocytes of the MT-4 (human T-cell leukemia) line and normal lymphocytes of healthy donors served as cell targets. For photodynamic treatment, a semiconductor laser was exploited as a light source, and cell viability was assessed by MTT or trypan blue dye exclusion tests. For cell imaging and HSA NPs visualization, the fluorescence microscopy and transmission electron microscopy were applied, respectively. C57Bl/6 mice were used in animal experiments. Results: The absorption and fluorescence spectra of chlorin e6-HSA NPs composites were characterized, and by the electron microscopy investigation the size of NPs (nanospheres) was estimated: 100–120 nm. FITC-labled albumin preparations allowed to establish that HSA NPs have much higher exposition and concentration dependent affinity to malignant cell surface than initial HSA. In experiments with MT-4 cells on PDT activity of chlorin e6-HSA NPs, the nanocomposite effectiveness elevated along with increasing percentage of cross-linked aminoacid residues, and for the nanocomposite with 100% of albumin cross-linking it exceeded the activity of free chlorin e6. In contrast to malignant cells, the complexation of chlorin e6 with HSA NPs decreased its photodynamic effect on normal human lymphocytes. Intravenous introduction of the chlorin e6-HSA NPs composite to mice showed prolonged circulation of the nanocomposite in blood in comparison with free PS. Conclusion: Promising results obtained with chlorin e6-HSA NPs composites warrant conduction of full-fledged PDT studies in vivo using the nanocomposites as photosensitizers. Key Words: human serum albumin, nanoparticles, photodynamic therapy, chlorin e6, cell line MT-4, C57Bl/6 mice.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145542
citation_txt Chlorin e6 combined with albumin nanoparticles as a potential composite photosensitizer for photodynamic therapy of tumors / I.O. Shton, V.V. Sarnatskaya, I.V. Prokopenko, N.F. Gamaleia // Experimental Oncology. — 2015. — Т. 37, № 4. — С. 250-254. — Бібліогр.: 24 назв. — англ.
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AT prokopenkoiv chlorine6combinedwithalbuminnanoparticlesasapotentialcompositephotosensitizerforphotodynamictherapyoftumors
AT gamaleianf chlorine6combinedwithalbuminnanoparticlesasapotentialcompositephotosensitizerforphotodynamictherapyoftumors
first_indexed 2025-12-01T09:30:28Z
last_indexed 2025-12-01T09:30:28Z
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