The study of mismatch repair in endometrial cancer patients with a family history of cancer
Aim: To assess the expression of mismatch repair (MMR) proteins MSH2 and MLH1 and carry out microsatellite analysis in patients with endometrial cancer (EC) with regard to the family history of cancer. Materials and Methods: Morphological and immunohistochemical study was performed on tumor tissue s...
Saved in:
| Published in: | Experimental Oncology |
|---|---|
| Date: | 2015 |
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2015
|
| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/145555 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | The study of mismatch repair in endometrial cancer patients with a family history of cancer / L.G. Buchynska, O.V. Brieieva, K.A. Nekrasov, S.V. Nespryadko // Experimental Oncology. — 2015. — Т. 37, № 4. — С. 272-276. — Бібліогр.: 33 назв. — англ. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862720565241970688 |
|---|---|
| author | Buchynska, L.G. Brieieva, O.V. Nekrasov, K.A. Nespryadko, S.V. |
| author_facet | Buchynska, L.G. Brieieva, O.V. Nekrasov, K.A. Nespryadko, S.V. |
| citation_txt | The study of mismatch repair in endometrial cancer patients with a family history of cancer / L.G. Buchynska, O.V. Brieieva, K.A. Nekrasov, S.V. Nespryadko // Experimental Oncology. — 2015. — Т. 37, № 4. — С. 272-276. — Бібліогр.: 33 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To assess the expression of mismatch repair (MMR) proteins MSH2 and MLH1 and carry out microsatellite analysis in patients with endometrial cancer (EC) with regard to the family history of cancer. Materials and Methods: Morphological and immunohistochemical study was performed on tumor tissue samples of 49 EC patients. Microsatellite instability was determined using PCR with primers which flank microsatellite region BAT-26. Results: A tendency to a decreased expression of both MSH2 and MLH1 markers in a group of EC patients with a family history of cancer as compared with a group without aggregation of cancer in family history was observed (labeling index — LI — was 36.1 ± 8.1% and LI 20.7 ± 9.1% versus LI 48.0 ± 5.8% and 33.8 ± 5.8%, respectively). It was determined that the number of EC patients with tumors deficient by expression of MMR markers was reliably higher in a group of patients with a family history of cancer than in a group of patients without aggregation of cancer in family history (р < 0.05). It was shown that in a group of EC patients with a family history of cancer, MMR-proficient tumors were detected in 38.5% of cases. Microsatellite instability was determined in 10.7% of EC patients including one patient with aggregation of Lynch-associated tumors in family history. Conclusion: Family history of cancer of EC patients is associated with malfunctioning of the MMR system as well as may be related to alternative molecular mechanisms. Key Words: endometrial cancer, mismatch repair, microsatellite instability, family history of cancer, Lynch syndrome.
|
| first_indexed | 2025-12-07T18:26:28Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145555 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T18:26:28Z |
| publishDate | 2015 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Buchynska, L.G. Brieieva, O.V. Nekrasov, K.A. Nespryadko, S.V. 2019-01-23T18:23:28Z 2019-01-23T18:23:28Z 2015 The study of mismatch repair in endometrial cancer patients with a family history of cancer / L.G. Buchynska, O.V. Brieieva, K.A. Nekrasov, S.V. Nespryadko // Experimental Oncology. — 2015. — Т. 37, № 4. — С. 272-276. — Бібліогр.: 33 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145555 Aim: To assess the expression of mismatch repair (MMR) proteins MSH2 and MLH1 and carry out microsatellite analysis in patients with endometrial cancer (EC) with regard to the family history of cancer. Materials and Methods: Morphological and immunohistochemical study was performed on tumor tissue samples of 49 EC patients. Microsatellite instability was determined using PCR with primers which flank microsatellite region BAT-26. Results: A tendency to a decreased expression of both MSH2 and MLH1 markers in a group of EC patients with a family history of cancer as compared with a group without aggregation of cancer in family history was observed (labeling index — LI — was 36.1 ± 8.1% and LI 20.7 ± 9.1% versus LI 48.0 ± 5.8% and 33.8 ± 5.8%, respectively). It was determined that the number of EC patients with tumors deficient by expression of MMR markers was reliably higher in a group of patients with a family history of cancer than in a group of patients without aggregation of cancer in family history (р < 0.05). It was shown that in a group of EC patients with a family history of cancer, MMR-proficient tumors were detected in 38.5% of cases. Microsatellite instability was determined in 10.7% of EC patients including one patient with aggregation of Lynch-associated tumors in family history. Conclusion: Family history of cancer of EC patients is associated with malfunctioning of the MMR system as well as may be related to alternative molecular mechanisms. Key Words: endometrial cancer, mismatch repair, microsatellite instability, family history of cancer, Lynch syndrome. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions The study of mismatch repair in endometrial cancer patients with a family history of cancer Article published earlier |
| spellingShingle | The study of mismatch repair in endometrial cancer patients with a family history of cancer Buchynska, L.G. Brieieva, O.V. Nekrasov, K.A. Nespryadko, S.V. Original contributions |
| title | The study of mismatch repair in endometrial cancer patients with a family history of cancer |
| title_full | The study of mismatch repair in endometrial cancer patients with a family history of cancer |
| title_fullStr | The study of mismatch repair in endometrial cancer patients with a family history of cancer |
| title_full_unstemmed | The study of mismatch repair in endometrial cancer patients with a family history of cancer |
| title_short | The study of mismatch repair in endometrial cancer patients with a family history of cancer |
| title_sort | study of mismatch repair in endometrial cancer patients with a family history of cancer |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145555 |
| work_keys_str_mv | AT buchynskalg thestudyofmismatchrepairinendometrialcancerpatientswithafamilyhistoryofcancer AT brieievaov thestudyofmismatchrepairinendometrialcancerpatientswithafamilyhistoryofcancer AT nekrasovka thestudyofmismatchrepairinendometrialcancerpatientswithafamilyhistoryofcancer AT nespryadkosv thestudyofmismatchrepairinendometrialcancerpatientswithafamilyhistoryofcancer AT buchynskalg studyofmismatchrepairinendometrialcancerpatientswithafamilyhistoryofcancer AT brieievaov studyofmismatchrepairinendometrialcancerpatientswithafamilyhistoryofcancer AT nekrasovka studyofmismatchrepairinendometrialcancerpatientswithafamilyhistoryofcancer AT nespryadkosv studyofmismatchrepairinendometrialcancerpatientswithafamilyhistoryofcancer |