The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium
The hyperplastic processes of the endometrium can arise not only against the background of excessive influence of estrogen, but also against the background of epigenetic damages that affect apoptosis, cell proliferation, differentiation, and adhesion, and DNA reparation. The aim of our study was to...
Збережено в:
| Опубліковано в: : | Experimental Oncology |
|---|---|
| Дата: | 2018 |
| Автори: | , , , , , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2018
|
| Теми: | |
| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/145574 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium / V.G. Marichereda, N.A. Bykovа, V.V. Bubnov, G.S. Manasova, T.Y. Moskalenko, A.G. Volyanska, I.M. Shevchenko, T.M. Adamovska // Experimental Oncology. — 2018 — Т. 40, № 2. — С. 109–113. — Бібліогр.: 21 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862528817522802688 |
|---|---|
| author | Marichereda, V.G. Bykovа, N.A. Bubnov, V.V. Manasova, G.S. Moskalenko, T.Y. Volyanska, A.G. Shevchenko, I.M. Adamovska, T.M. |
| author_facet | Marichereda, V.G. Bykovа, N.A. Bubnov, V.V. Manasova, G.S. Moskalenko, T.Y. Volyanska, A.G. Shevchenko, I.M. Adamovska, T.M. |
| citation_txt | The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium / V.G. Marichereda, N.A. Bykovа, V.V. Bubnov, G.S. Manasova, T.Y. Moskalenko, A.G. Volyanska, I.M. Shevchenko, T.M. Adamovska // Experimental Oncology. — 2018 — Т. 40, № 2. — С. 109–113. — Бібліогр.: 21 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | The hyperplastic processes of the endometrium can arise not only against the background of excessive influence of estrogen, but also against the background of epigenetic damages that affect apoptosis, cell proliferation, differentiation, and adhesion, and DNA reparation. The aim of our study was to investigate and analyze the status of methylation of the promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium. Materials and Methods: The study groups were the following: I — patients with endometrial hyperplasia (EH, n = 9); II — patients with endometrial intraepithelial neoplasia (EIN, n = 10), III — control groups: 1) with endometrial cancer (EC, n = 4), and 2) healthy women (n = 4). Determination of promoter methylation of SFRP2 gene was carried out by the semiquantitative method of methylation-specific PCR assay. Results: The maximum level of methylation of SFRP2 gene promoter had been revealed in patients with EC — 42.80 ± 3.55% (р < 0.05). The patients of the I group had the lowest values of methylation of SFRP2 gene promoter — 10.66 ± 0.85%, while in patients of the II group this indicator was higher — 20.60 ± 0.95% (р < 0.05). In healthy women of the control group, methylation of SFRP2 gene promoter was detected in none of the samples. Conclusion: The content of the methylated SFRP2 gene in endometrial tissue of patients with hyperplastic processes higher than 20–25% allows relate these women to the risk group of EC development and dictates the need of intensive observation of such patients. Key Words: methylation, SFRP2 gene, hyperplastic processes of the endometrium.
|
| first_indexed | 2025-11-24T02:38:48Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145574 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-24T02:38:48Z |
| publishDate | 2018 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Marichereda, V.G. Bykovа, N.A. Bubnov, V.V. Manasova, G.S. Moskalenko, T.Y. Volyanska, A.G. Shevchenko, I.M. Adamovska, T.M. 2019-01-24T08:40:07Z 2019-01-24T08:40:07Z 2018 The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium / V.G. Marichereda, N.A. Bykovа, V.V. Bubnov, G.S. Manasova, T.Y. Moskalenko, A.G. Volyanska, I.M. Shevchenko, T.M. Adamovska // Experimental Oncology. — 2018 — Т. 40, № 2. — С. 109–113. — Бібліогр.: 21 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145574 The hyperplastic processes of the endometrium can arise not only against the background of excessive influence of estrogen, but also against the background of epigenetic damages that affect apoptosis, cell proliferation, differentiation, and adhesion, and DNA reparation. The aim of our study was to investigate and analyze the status of methylation of the promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium. Materials and Methods: The study groups were the following: I — patients with endometrial hyperplasia (EH, n = 9); II — patients with endometrial intraepithelial neoplasia (EIN, n = 10), III — control groups: 1) with endometrial cancer (EC, n = 4), and 2) healthy women (n = 4). Determination of promoter methylation of SFRP2 gene was carried out by the semiquantitative method of methylation-specific PCR assay. Results: The maximum level of methylation of SFRP2 gene promoter had been revealed in patients with EC — 42.80 ± 3.55% (р < 0.05). The patients of the I group had the lowest values of methylation of SFRP2 gene promoter — 10.66 ± 0.85%, while in patients of the II group this indicator was higher — 20.60 ± 0.95% (р < 0.05). In healthy women of the control group, methylation of SFRP2 gene promoter was detected in none of the samples. Conclusion: The content of the methylated SFRP2 gene in endometrial tissue of patients with hyperplastic processes higher than 20–25% allows relate these women to the risk group of EC development and dictates the need of intensive observation of such patients. Key Words: methylation, SFRP2 gene, hyperplastic processes of the endometrium. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium Article published earlier |
| spellingShingle | The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium Marichereda, V.G. Bykovа, N.A. Bubnov, V.V. Manasova, G.S. Moskalenko, T.Y. Volyanska, A.G. Shevchenko, I.M. Adamovska, T.M. Original contributions |
| title | The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium |
| title_full | The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium |
| title_fullStr | The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium |
| title_full_unstemmed | The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium |
| title_short | The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium |
| title_sort | analysis of methylation of dna promoter of sfrp2 gene in patients with hyperplastic processes of the endometrium |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145574 |
| work_keys_str_mv | AT maricheredavg theanalysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT bykovana theanalysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT bubnovvv theanalysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT manasovags theanalysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT moskalenkoty theanalysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT volyanskaag theanalysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT shevchenkoim theanalysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT adamovskatm theanalysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT maricheredavg analysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT bykovana analysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT bubnovvv analysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT manasovags analysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT moskalenkoty analysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT volyanskaag analysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT shevchenkoim analysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium AT adamovskatm analysisofmethylationofdnapromoterofsfrp2geneinpatientswithhyperplasticprocessesoftheendometrium |