Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles
Aim: To identify gene expression profiles involved in drug resistance of different morphological structures (tubular, alveolar, solid, trabecular, and discrete) presented in breast cancer. Material and Methods: Ten patients with luminal breast cancer have been included. A laser microdissection-assis...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2018 |
| Hauptverfasser: | , , , , , , , , |
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| Sprache: | Englisch |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2018
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles / T.S. Gerashchenko, E.V. Denisov, N.M. Novikov, L.A. Tashireva, E.V. Kaigorodova, O.E. Savelieva, M.V. Zavyalova, N.V. Cherdyntseva, V.M. Perelmuter // Experimental Oncology. — 2018 — Т. 40, № 2. — С. 228–234. — Бібліогр.: 37 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862686881965146112 |
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| author | Gerashchenko, T.S. Denisov, E.V. Novikov, N.M. Tashireva, L.A. Kaigorodova, E.V. Savelieva, O.E. Zavyalova, M.V. Cherdyntseva, N.V. Perelmuter, V.M. |
| author_facet | Gerashchenko, T.S. Denisov, E.V. Novikov, N.M. Tashireva, L.A. Kaigorodova, E.V. Savelieva, O.E. Zavyalova, M.V. Cherdyntseva, N.V. Perelmuter, V.M. |
| citation_txt | Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles / T.S. Gerashchenko, E.V. Denisov, N.M. Novikov, L.A. Tashireva, E.V. Kaigorodova, O.E. Savelieva, M.V. Zavyalova, N.V. Cherdyntseva, V.M. Perelmuter // Experimental Oncology. — 2018 — Т. 40, № 2. — С. 228–234. — Бібліогр.: 37 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To identify gene expression profiles involved in drug resistance of different morphological structures (tubular, alveolar, solid, trabecular, and discrete) presented in breast cancer. Material and Methods: Ten patients with luminal breast cancer have been included. A laser microdissection-assisted microarrays and qRT-PCR were used to perform whole-transcriptome profiling of different morphological structures, to select differentially expressed drug response genes, and to validate their expression. Results: We found 27 differentially expressed genes (p < 0.05) encoding drug uptake (SLC1A3, SLC23A2, etc.) and efflux (ABCC1, ABCG1, etc.) transporters, drug targets (TOP2A, TYMS, and Tubb3), and proteins that are involved in drug detoxification (NAT1 and ALDH1B1), cell cycle progression (CCND1, AKT1, etc.), apoptosis (CASP3, TXN2, etc.), and DNA repair (BRCA1 and USP11). Each type of structures showed an individual gene expression profile related to resistance and sensitivity to anticancer drugs. However, most of the genes (19/27; p < 0.05) were expressed in alveolar structures. Functional enrichment analysis showed that drug resistance is significantly associated with alveolar structures. Other structures demonstrated the similar number (10–13 out of 27) of expressed genes; however, the spectrum of resistance and sensitivity to different anticancer drugs varied. Conclusion: Different morphological structures of breast cancer show individual expression of drug resistance genes. Key Words: breast cancer, tumor heterogeneity, gene expression, chemotherapy, drug resistance.
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| first_indexed | 2025-12-07T16:05:22Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-145587 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T16:05:22Z |
| publishDate | 2018 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Gerashchenko, T.S. Denisov, E.V. Novikov, N.M. Tashireva, L.A. Kaigorodova, E.V. Savelieva, O.E. Zavyalova, M.V. Cherdyntseva, N.V. Perelmuter, V.M. 2019-01-24T09:51:46Z 2019-01-24T09:51:46Z 2018 Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles / T.S. Gerashchenko, E.V. Denisov, N.M. Novikov, L.A. Tashireva, E.V. Kaigorodova, O.E. Savelieva, M.V. Zavyalova, N.V. Cherdyntseva, V.M. Perelmuter // Experimental Oncology. — 2018 — Т. 40, № 2. — С. 228–234. — Бібліогр.: 37 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/145587 Aim: To identify gene expression profiles involved in drug resistance of different morphological structures (tubular, alveolar, solid, trabecular, and discrete) presented in breast cancer. Material and Methods: Ten patients with luminal breast cancer have been included. A laser microdissection-assisted microarrays and qRT-PCR were used to perform whole-transcriptome profiling of different morphological structures, to select differentially expressed drug response genes, and to validate their expression. Results: We found 27 differentially expressed genes (p < 0.05) encoding drug uptake (SLC1A3, SLC23A2, etc.) and efflux (ABCC1, ABCG1, etc.) transporters, drug targets (TOP2A, TYMS, and Tubb3), and proteins that are involved in drug detoxification (NAT1 and ALDH1B1), cell cycle progression (CCND1, AKT1, etc.), apoptosis (CASP3, TXN2, etc.), and DNA repair (BRCA1 and USP11). Each type of structures showed an individual gene expression profile related to resistance and sensitivity to anticancer drugs. However, most of the genes (19/27; p < 0.05) were expressed in alveolar structures. Functional enrichment analysis showed that drug resistance is significantly associated with alveolar structures. Other structures demonstrated the similar number (10–13 out of 27) of expressed genes; however, the spectrum of resistance and sensitivity to different anticancer drugs varied. Conclusion: Different morphological structures of breast cancer show individual expression of drug resistance genes. Key Words: breast cancer, tumor heterogeneity, gene expression, chemotherapy, drug resistance. The study was supported by the Russian Science Foundation (grant # 14-15-00318). en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Reviews Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles Article published earlier |
| spellingShingle | Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles Gerashchenko, T.S. Denisov, E.V. Novikov, N.M. Tashireva, L.A. Kaigorodova, E.V. Savelieva, O.E. Zavyalova, M.V. Cherdyntseva, N.V. Perelmuter, V.M. Reviews |
| title | Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles |
| title_full | Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles |
| title_fullStr | Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles |
| title_full_unstemmed | Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles |
| title_short | Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles |
| title_sort | different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles |
| topic | Reviews |
| topic_facet | Reviews |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/145587 |
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