Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro

Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro

Aim: To investigate the biological effects of exogenous lactoferrin (LF) on phenotypic profile and invasiveness of human prostate cancer (PC) cells in vitro. Materials and Methods: Human PC cell lines (LNCaP, DU-145) were cultured with an exogenous LF at a dose corresponding to IC30. The expression...

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Veröffentlicht in:Experimental Oncology
Datum:2018
Hauptverfasser: Zadvornyi, T.V., Lukianova, N.Y., Borikun, T.V., Chekhun, V.F.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2018
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/145599
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Zitieren:Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro / T.V. Zadvornyi, N.Y. Lukianova, T.V. Borikun, V.F. Chekhun // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 184–189. — Бібліогр.: 36 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145599
record_format dspace
spelling Zadvornyi, T.V.
Lukianova, N.Y.
Borikun, T.V.
Chekhun, V.F.
2019-01-24T16:12:19Z
2019-01-24T16:12:19Z
2018
Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro / T.V. Zadvornyi, N.Y. Lukianova, T.V. Borikun, V.F. Chekhun // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 184–189. — Бібліогр.: 36 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145599
Aim: To investigate the biological effects of exogenous lactoferrin (LF) on phenotypic profile and invasiveness of human prostate cancer (PC) cells in vitro. Materials and Methods: Human PC cell lines (LNCaP, DU-145) were cultured with an exogenous LF at a dose corresponding to IC30. The expression levels of steroid hormone receptors (androgen receptor, estrogen receptor, progesterone receptor), Her2/neu, Ki-67, E- and N-cadherin, were monitored by immunohistochemical analysis. The levels of miRNAs were assessed using q-PCR. The invasive activity of the cells was examined in a standard invasion test. Results: Exogenous LF reduced expression of steroid hormone receptors (ERα and PR) and Ki-67 in both PC cell lines. The expression of E-cadherin increased significantly in LF-treated DU-145 cells. Also, we established the decrease in invasive activity upon LF treatment by 40% and 30% in DU-145 and LNCaP cells, respectively. In DU-145 cells, incubation with exogenous LF resulted in an increase in the expression of oncosuppressive (miR-133a and miR-200b) miRNAs. Conclusions: Exogenous LF causes the changes in phenotypic characteristics of PC cells and levels of oncogenic and oncosuppressive miRNAs involved in the regulation of key cellular processes. Key Words: prostate cancer, exogenous lactoferrin, DU145, LNCaP, miRNA.
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro
spellingShingle Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro
Zadvornyi, T.V.
Lukianova, N.Y.
Borikun, T.V.
Chekhun, V.F.
Original contributions
title_short Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro
title_full Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro
title_fullStr Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro
title_full_unstemmed Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro
title_sort effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (du145 and lncap) in vitro
author Zadvornyi, T.V.
Lukianova, N.Y.
Borikun, T.V.
Chekhun, V.F.
author_facet Zadvornyi, T.V.
Lukianova, N.Y.
Borikun, T.V.
Chekhun, V.F.
topic Original contributions
topic_facet Original contributions
publishDate 2018
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To investigate the biological effects of exogenous lactoferrin (LF) on phenotypic profile and invasiveness of human prostate cancer (PC) cells in vitro. Materials and Methods: Human PC cell lines (LNCaP, DU-145) were cultured with an exogenous LF at a dose corresponding to IC30. The expression levels of steroid hormone receptors (androgen receptor, estrogen receptor, progesterone receptor), Her2/neu, Ki-67, E- and N-cadherin, were monitored by immunohistochemical analysis. The levels of miRNAs were assessed using q-PCR. The invasive activity of the cells was examined in a standard invasion test. Results: Exogenous LF reduced expression of steroid hormone receptors (ERα and PR) and Ki-67 in both PC cell lines. The expression of E-cadherin increased significantly in LF-treated DU-145 cells. Also, we established the decrease in invasive activity upon LF treatment by 40% and 30% in DU-145 and LNCaP cells, respectively. In DU-145 cells, incubation with exogenous LF resulted in an increase in the expression of oncosuppressive (miR-133a and miR-200b) miRNAs. Conclusions: Exogenous LF causes the changes in phenotypic characteristics of PC cells and levels of oncogenic and oncosuppressive miRNAs involved in the regulation of key cellular processes. Key Words: prostate cancer, exogenous lactoferrin, DU145, LNCaP, miRNA.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145599
citation_txt Effects of exogenous lactoferrin on phenotypic profile and invasiveness of human prostate cancer cells (DU145 and LNCaP) in vitro / T.V. Zadvornyi, N.Y. Lukianova, T.V. Borikun, V.F. Chekhun // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 184–189. — Бібліогр.: 36 назв. — англ.
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first_indexed 2025-12-07T16:10:35Z
last_indexed 2025-12-07T16:10:35Z
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