Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles

Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles

Aim: To identify gene expression profiles involved in drug resistance of different morphological structures (tubular, alveolar, solid, trabecular, and discrete) presented in breast cancer. Material and Methods: Ten patients with luminal breast cancer have been included. A laser microdissection-assis...

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Veröffentlicht in:Experimental Oncology
Datum:2018
Hauptverfasser: Gerashchenko, T.S., Denisov, E.V., Novikov, N.M., Tashireva, L.A., Kaigorodova, E.V., Savelieva, O.E., Zavyalova, M.V., Cherdyntseva, N.V., Perelmuter, V.M.
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Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2018
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/145631
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Zitieren:Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles / T.S. Gerashchenko, E.V. Denisov, N.M. Novikov, L.A. Tashireva, E.V. Kaigorodova, O.E. Savelieva, M.V. Zavyalova, N.V. Cherdyntseva, V.M. Perelmuter // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 228–234. — Бібліогр.: 37 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-145631
record_format dspace
spelling Gerashchenko, T.S.
Denisov, E.V.
Novikov, N.M.
Tashireva, L.A.
Kaigorodova, E.V.
Savelieva, O.E.
Zavyalova, M.V.
Cherdyntseva, N.V.
Perelmuter, V.M.
2019-01-25T15:43:11Z
2019-01-25T15:43:11Z
2018
Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles / T.S. Gerashchenko, E.V. Denisov, N.M. Novikov, L.A. Tashireva, E.V. Kaigorodova, O.E. Savelieva, M.V. Zavyalova, N.V. Cherdyntseva, V.M. Perelmuter // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 228–234. — Бібліогр.: 37 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/145631
Aim: To identify gene expression profiles involved in drug resistance of different morphological structures (tubular, alveolar, solid, trabecular, and discrete) presented in breast cancer. Material and Methods: Ten patients with luminal breast cancer have been included. A laser microdissection-assisted microarrays and qRT-PCR were used to perform whole-transcriptome profiling of different morphological structures, to select differentially expressed drug response genes, and to validate their expression. Results: We found 27 differentially expressed genes (p < 0.05) encoding drug uptake (SLC1A3, SLC23A2, etc.) and efflux (ABCC1, ABCG1, etc.) transporters, drug targets (TOP2A, TYMS, and Tubb3), and proteins that are involved in drug detoxification (NAT1 and ALDH1B1), cell cycle progression (CCND1, AKT1, etc.), apoptosis (CASP3, TXN2, etc.), and DNA repair (BRCA1 and USP11). Each type of structures showed an individual gene expression profile related to resistance and sensitivity to anticancer drugs. However, most of the genes (19/27; p < 0.05) were expressed in alveolar structures. Functional enrichment analysis showed that drug resistance is significantly associated with alveolar structures. Other structures demonstrated the similar number (10–13 out of 27) of expressed genes; however, the spectrum of resistance and sensitivity to different anticancer drugs varied. Conclusion: Different morphological structures of breast cancer show individual expression of drug resistance genes. Key Words: breast cancer, tumor heterogeneity, gene expression, chemotherapy, drug resistance.
The study was supported by the Russian Science Foundation (grant # 14-15-00318).
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Reviews
Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles
spellingShingle Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles
Gerashchenko, T.S.
Denisov, E.V.
Novikov, N.M.
Tashireva, L.A.
Kaigorodova, E.V.
Savelieva, O.E.
Zavyalova, M.V.
Cherdyntseva, N.V.
Perelmuter, V.M.
Reviews
title_short Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles
title_full Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles
title_fullStr Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles
title_full_unstemmed Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles
title_sort different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles
author Gerashchenko, T.S.
Denisov, E.V.
Novikov, N.M.
Tashireva, L.A.
Kaigorodova, E.V.
Savelieva, O.E.
Zavyalova, M.V.
Cherdyntseva, N.V.
Perelmuter, V.M.
author_facet Gerashchenko, T.S.
Denisov, E.V.
Novikov, N.M.
Tashireva, L.A.
Kaigorodova, E.V.
Savelieva, O.E.
Zavyalova, M.V.
Cherdyntseva, N.V.
Perelmuter, V.M.
topic Reviews
topic_facet Reviews
publishDate 2018
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To identify gene expression profiles involved in drug resistance of different morphological structures (tubular, alveolar, solid, trabecular, and discrete) presented in breast cancer. Material and Methods: Ten patients with luminal breast cancer have been included. A laser microdissection-assisted microarrays and qRT-PCR were used to perform whole-transcriptome profiling of different morphological structures, to select differentially expressed drug response genes, and to validate their expression. Results: We found 27 differentially expressed genes (p < 0.05) encoding drug uptake (SLC1A3, SLC23A2, etc.) and efflux (ABCC1, ABCG1, etc.) transporters, drug targets (TOP2A, TYMS, and Tubb3), and proteins that are involved in drug detoxification (NAT1 and ALDH1B1), cell cycle progression (CCND1, AKT1, etc.), apoptosis (CASP3, TXN2, etc.), and DNA repair (BRCA1 and USP11). Each type of structures showed an individual gene expression profile related to resistance and sensitivity to anticancer drugs. However, most of the genes (19/27; p < 0.05) were expressed in alveolar structures. Functional enrichment analysis showed that drug resistance is significantly associated with alveolar structures. Other structures demonstrated the similar number (10–13 out of 27) of expressed genes; however, the spectrum of resistance and sensitivity to different anticancer drugs varied. Conclusion: Different morphological structures of breast cancer show individual expression of drug resistance genes. Key Words: breast cancer, tumor heterogeneity, gene expression, chemotherapy, drug resistance.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/145631
citation_txt Different morphological structures of breast tumors demonstrate individual drug resistance gene expression profiles / T.S. Gerashchenko, E.V. Denisov, N.M. Novikov, L.A. Tashireva, E.V. Kaigorodova, O.E. Savelieva, M.V. Zavyalova, N.V. Cherdyntseva, V.M. Perelmuter // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 228–234. — Бібліогр.: 37 назв. — англ.
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first_indexed 2025-12-07T21:13:00Z
last_indexed 2025-12-07T21:13:00Z
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